- Clone
- A15144A (See other available formats)
- Regulatory Status
- RUO
- Other Names
- SYM1, SYNS1, NOG, Symphalangism 1, SYNS1A
- Isotype
- Mouse IgG2b, κ
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Recombinant Human Noggin (Cat. No. 795904) (black circles) inhibits Recombinant Human BMP-4 (Cat. No. 795604) induced alkaline phosphatase production of ATDC5 mouse chondrogenic cells. Ultra-LEAF™ purified anti-human Noggin (clone A15144A) (purple squares) neutralizes the effect of recombinant human Noggin at 0.5 µg/mL and human BMP-4 at 50 ng/mL in a dose-dependent manner, whereas the Ultra-LEAF™ purified mouse IgG2b, κ isotype control (Cat. No. 401216) (gray triangles) does not have this effect. ND50 range: 0.3-2 µg/mL
| Cat # | Size | Price | Quantity Check Availability | ||
|---|---|---|---|---|---|
| 620453 | 100 µg | $380.00 | |||
| 620454 | 1 mg | $1225.00 | |||
Select size of product is eligible for a 40% discount! Promotion valid until December 31, 2024. Exclusions apply. To view full promotion terms and conditions or to contact your local BioLegend representative to receive a quote, visit our webpage.
Noggin (NOG) is an embryonic inducer that can sequester TGF-β cytokines of the BMP family with extremely high affinity. This protein was originally identified as a BMP-4 antagonist whose action is critical for proper formation of the head and other dorsal structures. NOG also has been shown to modulate the activities of other BMPs including BMP-2, -7, -13, and -14. It is expressed in defined areas of the adult central nervous system and peripheral tissues such as lung, skeletal muscle, and skin. During culture of human embryonic stem cells (human ESC) or neural stem cells under certain conditions, addition of NOG to antagonize BMP activity may allow stem cells to proliferate while maintaining their undifferentiated state, or alternatively, to differentiate into dopaminergic neurons. NOG also appears to maintain adult stem cell populations in vivo. For example, it maintains neural stem cells within the hippocampus. Targeted deletion of NOG in mice results in prenatal death and recessive phenotype displaying a severely malformed skeletal system. Conversely, transgenic mice over-expressing NOG in mature osteoblasts display impaired osteoblastic differentiation, reduced bone formation, and severe osteoporosis.
Product Details
- Verified Reactivity
- Human
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- Recombinant human Noggin
- Formulation
- 0.2 µm filtered in phosphate-buffered solution, pH 7.2, containing no preservative.
- Preparation
- The Ultra-LEAF™ (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.
- Concentration
- The antibody is bottled at the concentration indicated on the vial, typically between 2 mg/mL and 3 mg/mL. To obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C. This Ultra-LEAF™ solution contains no preservative; handle under aseptic conditions.
- Application
-
Neut - Quality tested
- Recommended Usage
-
Each lot of this antibody is quality control tested by neutralizing alkaline phosphatase production of ATDC5 mouse chondrogenic cells which was inhibited by recombinant human Noggin (Cat. No. 795904) at 0.5 µg/mL and human BMP-4 (Cat. No. 795604) at 50 ng/ml. ND50 range: 0.3-2 µg/mL. It is recommended that the reagent be titrated for optimal performance for each application.
- RRID
-
AB_3083405 (BioLegend Cat. No. 620453)
AB_3083405 (BioLegend Cat. No. 620454)
Antigen Details
- Structure
- Cytokine
- Distribution
-
Skeletal muscles, lungs, neurons, and skin
- Function
- Antagonizes BMPs
- Interaction
- Mesenchymal stem cells
- Ligand/Receptor
- BMPs
- Cell Type
- Embryonic Stem Cells, Mesenchymal Stem Cells
- Biology Area
- Cell Biology, Immunology, Neuroscience, Signal Transduction, Stem Cells, Synaptic Biology
- Molecular Family
- Cytokines/Chemokines, Growth Factors
- Antigen References
-
- Valenzuela DM, et al. 1995. J Neurosci. 15:6077-84.
- McMahon JA, et al. 1998. Genes Dev. 12:1438-52.
- Yuasa S, et al. 2005. Nat Biotechnol. 23:607-11.
- Gong Y, et al. 1999. Nat Genet. 21:302-4.
- Chiba S, et al. 2008. Stem Cells. 26:2810-20.
- Bonaguidi MA, et al. 2008. J Neurosci. 28:9194-204.
- Gene ID
- 9241 View all products for this Gene ID
- UniProt
- View information about Noggin on UniProt.org
Other Formats
View All Noggin Reagents Request Custom Conjugation| Description | Clone | Applications |
|---|---|---|
| Ultra-LEAF™ Purified anti-human Noggin | A15144A | Neut |
Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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Ultra-LEAF™ Purified anti-human Noggin
Recombinant Human Noggin (Cat. No. 795904) (black circles) i...