- Clone
- W17087B (See other available formats)
- Regulatory Status
- RUO
- Other Names
- Neuroregulin 1, Neu differentiation factor (NDF), Acetylcholine receptor-inducing activity, chick, homolog of (ARIA), glial growth factor, HGL, HRG, HRG1
- Isotype
- Rat IgG2b, κ
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Recombinant human NRG1 (Heregulin) EGF Domain (Cat. No. 551904) (black circles) induces proliferation of MCF-7 cells. Ultra- LEAF™ purified anti-human NRG1b-EGF (clone W17087B) (purple squares) neutralizes MCF-7 cell proliferation induced by recombinant human NRG1 (Heregulin) EGF Domain at 10 ng/mL in a dose-dependent manner, whereas the Ultra-LEAF™ purified rat IgG2b, κ isotype control (Cat. No. 400644) (gray triangles) does not have the effect. ND50 range is 2 - 15 µg/mL.
| Cat # | Size | Price | Quantity Check Availability | ||
|---|---|---|---|---|---|
| 638653 | 100 µg | $470.00 | |||
| 638654 | 1 mg | $1175.00 | |||
Neuregulin 1 (NRG1) was initially identified in the conditioned medium of a human breast tumor cell line as a protein of 45 kD, which induces the phosphorylation of a tyrosine kinase (ERBB2). The neuregulin family includes four members (NRG1-NRG4) that are encoded from four individual genes. Through differential promoter usage and splicing, these members generate 12 isoforms. The best characterized is neuregulin 1, which includes eight isoforms; all isoforms contain (from N-terminus to C-terminus) an immunoglobulin-like (Ig) domain, a growth factor-like domain (EGF), a transmembrane (TM), and a cytoplasmic domain. Alternative splicing in the EGF-like domain of neuregulin 1 results in α and β isoforms. The EGF-like domain is necessary and sufficient for neuregulin bioactivity. The EGFR family of receptors includes four members (ErbB1 through ErbB4), and neuregulins bind to the ErbB3 and ErbB4 receptors. Neuregulins are released from the membrane by proteolytic cleavage, and this process is required for the binding of the neuregulin derived fragments to ErbB receptors. The neuregulin1-β isoform is predominant in the central nervous system and participates in development, survival, and metabolism in neuron and glial cells. Neuregulin1-β is neuroprotective and attenuates inflammatory responses induced by ischemic stroke in rats, preventing macrophage and microglial infiltration and astrocytic activation. Also, neuregulin1-β blocks the induction of pro-inflammatory and stress genes provoked by ischemia. Neuregulins 1-4 are expressed in approximately 25% of breast cancer carcinomas and increase breast cancer cell proliferation, increase tumorigenesis, and promote invasive characteristics of cancer cells. In this sense, neuregulin1-β induces MMP-1 and MMP-9 in cancer cell lines.
Product Details
- Verified Reactivity
- Human
- Antibody Type
- Monoclonal
- Host Species
- Rat
- Immunogen
- Recombinant Human NRG1 (Heregulin) EGF Domain
- Formulation
- 0.2 µm filtered in phosphate-buffered solution, pH 7.2, containing no preservative.
- Preparation
- The Ultra-LEAF™ (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.
- Concentration
- The antibody is bottled at the concentration indicated on the vial, typically between 2 mg/mL and 3 mg/mL. To obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C. This Ultra-LEAF™ solution contains no preservative; handle under aseptic conditions.
- Application
-
Neut - Quality tested
- Recommended Usage
-
Each lot of this antibody is quality control tested by neutralizing the proliferation of MCF-7 cells induced by recombinant human NRG1 (Heregulin) EGF Domain (Cat. No. 551904) at 10 ng/mL. ND50 range: 2 – 15 μg/mL. It is recommended that the reagent be titrated for optimal performance for each application.
- RRID
-
AB_3662321 (BioLegend Cat. No. 638653)
AB_3662321 (BioLegend Cat. No. 638654)
Antigen Details
- Structure
- Monomer
- Distribution
-
Gastric epithelial cells, breast cancer cells, fibroblasts, and Schwann cells
- Function
- Neuregulin 1 plays a key role in embryonic development of the nervous system, heart, and mammary glands. It also regulates the constitutive color and melanocyte function in human skin, and might maintain cardiac structure and function in adults.
- Interaction
- Melanocytes, melanoma cells, epithelial cells, gastric epithelial cells, mesenchymal cells, and Schwann cells
- Ligand/Receptor
- ErbB3-ErbB2 and ErbB3-ErbB4
- Cell Type
- Embryonic Stem Cells, Hematopoietic stem and progenitors, Mesenchymal Stem Cells, Neural Stem Cells
- Biology Area
- Cell Biology, Neuroscience, Stem Cells, Synaptic Biology
- Molecular Family
- Cytokines/Chemokines, Growth Factors
- Antigen References
-
- Holmes WE, et al. 1992. Sciences. 256:1205-10.
- Tan W, et al. 2007. J Biol Chem. 282:24343-51.
- Buac K, et al. 2009. Pigment Cell Melanoma Res. 22:773-84.
- Shamir A and Buonanno A. 2010. J Neurochem. 113:1163-76.
- Mei L and Xiong WC. 2008. Nat. Rev Neurosci. 9:437-52.
- Luo X, et al. 2011. J Biol Chem. 286:23967-74.
- Zhang K, et al. 2013. Pigment Cell Melanoma Res. 26:408-14.
- Gene ID
- 3084 View all products for this Gene ID
- UniProt
- View information about NRG1b-EGF on UniProt.org
Other Formats
View All Reagents Request Custom Conjugation| Description | Clone | Applications |
|---|---|---|
| Ultra-LEAF™ Purified anti-human NRG1b-EGF | W17087B | Neut |
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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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Ultra-LEAF™ Purified anti-human NRG1b-EGF
Recombinant human NRG1 (Heregulin) EGF Domain (Cat. No. 5519...