- Clone
- W22016A (See other available formats)
- Regulatory Status
- RUO
- Other Names
- Placental growth factor 1 (PGF1)
- Isotype
- Rat IgG2a, κ
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Recombinant human VEGFR1 (Cat. No. 555804) (black circles) binds to immobilized recombinant human PLGF (Cat. No. 590704). Ultra-LEAF™ purified anti-human PLGF (clone W22016A) (purple squares) inhibits the binding in a dose-dependent manner, whereas the Ultra-LEAF™ purified rat IgG2a, κ isotype control (Cat. No. 400544) (gray triangles) does not have the effect. This antibody blocks the binding of 0.25 µg/mL human VEGFR1 to 0.5 µg/mL immobilized recombinant human PLGF (Cat. No. 590704). ND50 range: 0.06 - 0.35 µg/mL.
| Cat # | Size | Price | Quantity Check Availability | ||
|---|---|---|---|---|---|
| 647653 | 100 µg | $405.00 | |||
| 647654 | 1 mg | $1045.00 | |||
Placenta growth factor (PLGF) was initially cloned form a term placenta cDNA library. PLGF is a dimeric glycoprotein with 53% homology to VEGF. As a result of alternative splicing, four isoforms for PLGF has been described, PLGF-1, PLGF-2, PLGF-3, and PLGF-4. PLGF-2 binds to heparin with high affinity, whereas neither PLGF-1, PLGF-3, nor PLGF-4 bind heparin. PLGF-2 is the only isoform in mouse. PLGF binds to VEGFRI but not to VEGFRII; also, PLGF binds to sVEGF1. PLGF plays a key role in the maternal vascular function during pregnancy. Low levels of PLGF have been associated with patients experiencing preeclampsia and delivering small-for-gestational age (SGA) neonates. High expression of PLGF has been associated to colorectal cancer (CRC), and its expression in CRC tissues correlates with high levels of MMP-9 and VEGF1. PLGF has been shown to increase tumor cell migration in lung cancer, leukemia, and melanoma cells. In colorectal cancer, PLGF induces tumor cell migration and invasion through upregulation of MMP9. PLGF is expressed in the majority of medulloblastomas, and high expression of PLGF receptor neuropilin 1 (Nrp1) correlates with poor survival in patients. In a mouse model, PLGF/Nrp1 blockade provided direct antitumor effects in vivo, medulloblastoma regression, decreased metastasis, and increased survival.
Product Details
- Verified Reactivity
- Human
- Antibody Type
- Monoclonal
- Host Species
- Rat
- Immunogen
- Recombinant human PLGF
- Formulation
- 0.2 µm filtered in phosphate-buffered solution, pH 7.2, containing no preservative.
- Preparation
- The Ultra-LEAF™ (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.
- Concentration
- The antibody is bottled at the concentration indicated on the vial, typically between 2 mg/mL and 3 mg/mL. To obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C. This Ultra-LEAF™ solution contains no preservative; handle under aseptic conditions.
- Application
-
Block - Quality tested
- Recommended Usage
-
Each lot of this antibody is quality control tested by blocking the binding of 0.25 µg/mL recombinant human VEGFR1 (Cat. No. 555804) to immobilized 0.5 µg/mL recombinant human PLGF (Cat. No. 590704). ND50 range: 0.06 - 0.35 µg/mL. It is recommended that the reagent be titrated for optimal performance for each application.
- RRID
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AB_3662330 (BioLegend Cat. No. 647653)
AB_3662330 (BioLegend Cat. No. 647654)
Antigen Details
- Structure
- Homodimer
- Distribution
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Vascular endothelial cells, smooth muscle cells, keratinocytes, hematopoitic cells, retinal pigment epithelial cells, tumor cells, and cerebellar stroma cells
- Function
- PLGF is an angiogenic factor and induces proliferation and migration of endothelial cells. It stimulates endothelial cells and synergistically amplifies the action of VEGF. Soluble VEGFR1 (sFLT1) can regulate the accessibility of PLGF.
- Interaction
- Endothelial cells, Macrophages, and trophoblast
- Ligand/Receptor
- VEGFR1 (FLT-1), and neuropilin 1 (Nrp1)
- Cell Type
- Hematopoietic stem and progenitors
- Biology Area
- Cell Biology, Signal Transduction, Stem Cells
- Molecular Family
- Cytokines/Chemokines, Growth Factors
- Antigen References
-
- Maglione D, et al. 1991. Proc Natl Acad Sci USA. 88:9267-71.
- Carmeliet P, et al. 2001. Nat Med. 7:575-83.
- Casalou C, et al. 2007. Leukemia. 21:1590-4.
- Marcellini M, et al. 2006. Am J Pathol. 169:643-54.
- Yao J, et al. 2011. Proc Natl Acad Sci USA. 108:11590.
- Cai J, et al. 2011. PLoS One. 6:e18076.
- Sibiude J, et al. 2012. PLoS One. 7:e50208.
- Snuderl M, et al. 2013. Cell. 152:1065-76.
- Gene ID
- 5228 View all products for this Gene ID
- UniProt
- View information about PLGF on UniProt.org
Other Formats
View All PLGF Reagents Request Custom Conjugation| Description | Clone | Applications |
|---|---|---|
| Ultra-LEAF™ purified anti-human PLGF | W22016A | Block |
Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
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Ultra-LEAF™ purified anti-human PLGF
Recombinant human VEGFR1 (Cat. No. 555804) (black circles) b...