- Clone
- W20296I (See other available formats)
- Regulatory Status
- RUO
- Other Names
- Thymic stromal lymphopoietin protein receptor, cytokine receptor-like 2, CRL2, CRLF2, IL-XR, TSLP-R
- Isotype
- Rat IgG2a, κ
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Recombinant human TSLP (Cat. No. 582402) (black circles) induces the proliferation of Baf3-hTSLPR/hIL-7Ra transfectant cells. Ultra-LEAF™ purified anti-human TSLPR (clone W20296I) (purple squares) neutralizes the proliferation of Baf3-hTSLPR/hIL-7Ra transfectant cells induced by recombinant human TSLP at 10 ng/mL in a dose-dependent manner, whereas the Ultra-LEAF™ purified rat IgG2a, κ isotype control (Cat. No. 400544) (gray triangles) does not have this effect. ND50 range: 0.6-4 µg/mL.
| Cat # | Size | Price | Quantity Check Availability | ||
|---|---|---|---|---|---|
| 622353 | 100 µg | $430.00 | |||
| 622354 | 1 mg | $1235.00 | |||
Select size of product is eligible for a 40% discount! Promotion valid until December 31, 2024. Exclusions apply. To view full promotion terms and conditions or to contact your local BioLegend representative to receive a quote, visit our webpage.
TSLP-R, also known as the thymic stromal lymphopoietin protein receptor, cytokine receptor-like 2, CRL2, and IL-XR, is a type I membrane receptor that forms a functional heterodimeric complex with IL-7R to bind with TSLP. TSLP-R contains a WSXWS motif required for proper protein folding and a box 1 motif important for association with the JAKs. TSLP-R has a predicted molecular weight of approximately 40 kD, and has two isoforms reported that are produced by alternative splicing. The TSLP-R is expressed preferentially in myeloid cells, dendritic cells, and activated monocytes. It is weakly expressed on T cells. Expression has also been reported in heart, skeletal, muscle, and kidney tissues. TSLP binding to the heterodimeric functional receptor (TSLP-R and IL-7R) activates JAK2, STAT3, and STAT5 to stimulate cell proliferation. Ligand receptor interactions have been implicated in the development of the hematopoietic system, dendritic cell maturation, and the maintenance and polarization of human Th2 memory T cells in an allergic response.
Product Details
- Verified Reactivity
- Human
- Antibody Type
- Monoclonal
- Host Species
- Rat
- Immunogen
- Recombinant human TSLPR
- Formulation
- 0.2 µm filtered in phosphate-buffered solution, pH 7.2, containing no preservative.
- Preparation
- The Ultra-LEAF™ (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.
- Concentration
- The antibody is bottled at the concentration indicated on the vial, typically between 2 mg/mL and 3 mg/mL. To obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C. This Ultra-LEAF™ solution contains no preservative; handle under aseptic conditions.
- Application
-
Neut - Quality tested
- Recommended Usage
-
Each lot of this antibody is quality control tested by neutralizing the proliferation of Baf3-hTSLPR/hIL-7Ra transfectant cells induced by recombinant human TSLP at 10 ng/mL. ND50 range: 0.6 - 4 μg/mL. It is recommended that the reagent be titrated for optimal performance for each application.
- RRID
-
AB_3083411 (BioLegend Cat. No. 622353)
AB_3083411 (BioLegend Cat. No. 622354)
Antigen Details
- Structure
- Type I membrane receptor
- Distribution
-
Expressed preferentially in myeloid cells including dendritic cells and activated monocytes, weakly expressed in T cells; also expressed in heart, skeletal, muscle, and kidney cells.
- Function
- TSLP binding to functional receptor activates JAK2, STAT3, and STAT5 to stimulate cell proliferation. It has also been implicated in the development of the hematopoietic system, dendritic cell maturation, and the maintenance and polarization of human Th2 memory T cells
- Interaction
- Forms functional heterodimeric complex with IL-7R to bind TSLP
- Ligand/Receptor
- TSLP/TSLPR
- Cell Type
- Dendritic cells, Monocytes, T cells, Tregs
- Biology Area
- Immunology
- Molecular Family
- Cytokine/Chemokine Receptors
- Antigen References
-
- Reche PA, et al. 2001. J Immunol. 167:336-43.
- Tonozuka Y, et al. 2001. Cytogenet. Cell. Genet. 93:23-5.
- Zhang W, et al. 2001. Biochem Biophys Res Commun. 281:878-83.
- Wang YH, et al. 2006. Immunity, 24:-838.
- Gene ID
- 64109 View all products for this Gene ID
- UniProt
- View information about TSLPR on UniProt.org
Other Formats
View All TSLPR Reagents Request Custom Conjugation| Description | Clone | Applications |
|---|---|---|
| Ultra-LEAF™ Purified anti-human TSLPR | W20296I | Neut |
Compare Data Across All Formats
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Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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Ultra-LEAF™ Purified anti-human TSLPR
Recombinant human TSLP (Cat. No. 582402) (black circles) ind...