- Clone
- W19361A (See other available formats)
- Regulatory Status
- RUO
- Other Names
- Mast/stem cell growth factor receptor Kit, SCFR, c-Kit, Kit, CD117
- Isotype
- Rat IgG2a, κ
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Recombinant mouse CD117 (c-kit)-Fc chimera (Cat. No. 790806) (black circles) binds to immobilized recombinant mouse SCF (Cat. No. 579706). Ultra-LEAF™ purified anti-mouse CD117 (clone W19361A) (purple squares) inhibits the binding in a dose-dependent manner, whereas the GoInVivo™ purified rat IgG2a, κ isotype control (Cat. No. 400562) (gray triangles) does not have the effect. This antibody blocks the binding of 1 µg/mL recombinant mouse CD117 (c-kit)-Fc chimera (Cat. No. 790806) to 2 µg/mL immobilized recombinant mouse SCF (Cat. No. 579706). ND50 range: 0.5 - 2 µg/mL.
| Cat # | Size | Price | Quantity Check Availability | ||
|---|---|---|---|---|---|
| 946803 | 100 µg | $171.00 | |||
| 946804 | 1 mg | $655.00 | |||
Select size of product is eligible for a 40% discount! Promotion valid until December 31, 2024. Exclusions apply. To view full promotion terms and conditions or to contact your local BioLegend representative to receive a quote, visit our webpage.
CD117 is a 145 kD protein tyrosine kinase also known as c-Kit and stem cell factor receptor (SCFR). It belongs to the CSF-1/PDGF receptor subfamily. CD117 is a receptor for stem cell factor (SCF) or c-Kit ligand. CD117 is expressed on pluripotent hematopoietic progenitor cells, mast cells, and acute myeloid leukemia cells (AML). c-Kit ligand binding of CD117 induces dimerization and phosphorylation of CD117, and activates the downstream signaling, including PI3K, JAK/STAT, PLC-γ and MAPK pathways. Consequently, it stimulates proliferation and survival of primitive hematopoietic stem cells as well as erythroid-committed and granulo-monocytic committed cells. The turnover rate of CD117 depends on ligand binding and its kinase activity. CD117 is also downregulated by PKC-mediated proteolytic cleavage. Additionlly, ADAM-17 mediates shedding of CD117 on the surface of mast cells. SCF/CD117 ligation protects vascular smooth musle cells against apoptosis and increases hyperplasia after vascular injury. The ligation also regulates cell survival and cell infiltration of mast cells in tumor microenviroment. Some evidence indicates that CD117 constitutively associates with interleukin-1 receptor accessory protein (IL-1RAcP), and activates IL-33-induced cytokine production upon costimulation with c-Kit ligand in primary mast cells. CD117 is upregulated on dendritic cells by house dust mite allergen and mucosal adjuvant cholera toxin (CT), and consequently regulates Th2's and Th17's responses.
Product Details
- Verified Reactivity
- Mouse
- Antibody Type
- Monoclonal
- Host Species
- Rat
- Immunogen
- Recombinant mouse CD117 (c-Kit)
- Formulation
- 0.2 µm filtered in phosphate-buffered solution, pH 7.2, containing no preservative.
- Preparation
- The Ultra-LEAF™ (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.
- Concentration
- The antibody is bottled at the concentration indicated on the vial, typically between 2 mg/mL and 3 mg/mL. Older lots may have also been bottled at 1 mg/mL. To obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C. This Ultra-LEAF™ solution contains no preservative; handle under aseptic conditions.
- Application
-
Block - Quality tested
- Recommended Usage
-
Each lot of this antibody is quality control tested by blocking the binding of 1 µg/mL recombinant mouse CD117 (c-Kit)-Fc chimera (Cat. No. 790806) to 2 µg/mL immobilized recombinant mouse SCF (Cat. No. 579706). ND50 range: 0.5 - 2 µg/mL. It is recommended that the reagent be titrated for optimal performance for each application.
- RRID
-
AB_2922647 (BioLegend Cat. No. 946803)
AB_2922647 (BioLegend Cat. No. 946804)
Antigen Details
- Structure
- Disulfide-linked homodimer
- Distribution
-
Hematopoietic stem cells, AML, mast cells
- Function
- Growth factor receptor, tyrosine kinase
- Interaction
- Endothelial cells, hematopoietic stem and progenitors, mast cells
- Ligand/Receptor
- Stem cell factor (SCF)
- Cell Type
- Embryonic Stem Cells, Mesenchymal Stem Cells
- Biology Area
- Immunology, Stem Cells
- Molecular Family
- CD Molecules
- Antigen References
-
- Nocka K, et al. 1990. EMBO J. 9:3287-94.
- Reith AD, et al. 1991. EMBO J. 9:2451-9.
- Cardoso HJ, et al. 2017. J Cell Commun Signal. 4:297-307.
- Sette C, et al. 1998. J Cell Biol. 142:1063-74.
- Kurosawa K, et al. 1996. Blood. 87:2235-43.
- Drube S, et al. 2010. Blood. 115:3899-906.
- Lemmon MA, et al. 1997. J Biol Chem. 272:6311-7.
- Yee NS, et al. 1994. J Biol Chem. 269:31991-8.
- Cruz AC, et al. 2004. J Biol Chem. 279:5612-20.
- Kimura Y, et al. 2011. PLoS One. 6:e26918.
- Krishnamoorthy N, et al. 2008. Nat Med. 14:565-73.
- Huang B, et al. 2008. Blood. 112:1269-79.
- Gene ID
- 16590 View all products for this Gene ID
- UniProt
- View information about CD117 on UniProt.org
Other Formats
View All CD117 Reagents Request Custom Conjugation| Description | Clone | Applications |
|---|---|---|
| Ultra-LEAF™ Purified anti-mouse CD117 (c-Kit) | W19361A | Block |
Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
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Ultra-LEAF™ Purified anti-mouse CD117 (c-Kit)
Recombinant mouse CD117 (c-kit)-Fc chimera (Cat. No. 790806)...