Ultra-LEAF™ Purified anti-mouse CD18 Antibody

Pricing & Availability
Clone
M18/2 (See other available formats)
Regulatory Status
RUO
Other Names
integrin β2, β2 integrin, ITGB2
Isotype
Rat IgG2a, κ
M18slash2_LEAF_080207
C57BL/6 mouse splenocytes stained with Ultra-LEAF™ purified M18/2, followed by anti-rat IgG FITC
  • M18slash2_LEAF_080207
    C57BL/6 mouse splenocytes stained with Ultra-LEAF™ purified M18/2, followed by anti-rat IgG FITC
Compare all formats
Cat # Size Price Quantity Check Availability
101417 100 µg $110.00
Check Availability


Need larger quantities of this item?
Request Bulk Quote
101418 1 mg $149.00
Check Availability


Need larger quantities of this item?
Request Bulk Quote
101419 5 mg $539.00
Check Availability


Need larger quantities of this item?
Request Bulk Quote
101420 25 mg $1408.00
Check Availability


Need larger quantities of this item?
Request Bulk Quote
101421 50 mg $2354.00
Check Availability


Need larger quantities of this item?
Request Bulk Quote
101422 100 mg $3383.00
Check Availability


Need larger quantities of this item?
Request Bulk Quote

Select size of product is eligible for a 40% discount! Promotion valid until December 31, 2024. Exclusions apply. To view full promotion terms and conditions or to contact your local BioLegend representative to receive a quote, visit our webpage.

Description

CD18 is a 95 kD protein, also known as integrin β2 subunit. It is expressed on all leukocytes. CD18, in association with integrin α chain CD11a, CD11b, and CD11c forms LFA-1, Mac-1, and αXβ2, respectively, and plays an important role in leukocytes adhesion. The CD18 integrin complexes bind ICAM-1 (CD54), ICAM-2 (CD102), ICAM-3 (CD50), iC3b, and fibrinogen. The M18/2 antibody is able to block tumor cell metastasis and enhance cell adhesion.

Technical data sheet

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Mouse CTL glycoprotein
Formulation
0.2 µm filtered in phosphate-buffered solution, pH 7.2, containing no preservative.
Concentration
The antibody is bottled at the concentration indicated on the vial, typically between 2 mg/mL and 3 mg/mL. Older lots may have also been bottled at 1 mg/mL. To obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C. This Ultra-LEAF™ solution contains no preservative; handle under aseptic conditions.
Application

FC - Quality tested
IP, WB, Block, Stim, IHC - Reported in the literature, not verified in house
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 1.0 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Additional reported applications (for the relevant formats) include: immunoprecipitation1,2, Western blotting1, in vitro and in vivo blocking of tumor cells metastasis or stimulation of cell-adhesion2-6, and immunohistochemical staining7 of acetone-fixed frozen sections and formalin-fixed paraffin-embedded sections. The Ultra-LEAF™ purified antibody (Endotoxin <0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 101417-101422).

Application References

(PubMed link indicates BioLegend citation)
  1. Sanchez-Madrid, et al. 1983. J. Exp. Med. 158:586. (IP, WB)
  2. Zahalka MA, et al. 1993. J. Immunol. 150:4466. (IP, Block, Stim)
  3. Zahalka MA, et al. 1994. Int. Immunol. 6:917. (Block, Stim)
  4. Ishida Y, et al. 1994. Cell. Immunol. 155:414. (Block, Stim)
  5. Isobe M, et al. 1992. Science 255:1125. (Block, Stim)
  6. Driessens MHE, et al. 1996. J. Leukoc. Biol. 60:758. (Block, Stim)
  7. Barlow SC, et al. 2004. Amer. J. Pathol. 165:1849. (IHC)
Product Citations
  1. Mysore V, et al. 2022. J Exp Med. 219:. PubMed
  2. Mattei F, et al. 2022. iScience. 25:105110. PubMed
RRID
AB_2832276 (BioLegend Cat. No. 101417)
AB_2832276 (BioLegend Cat. No. 101418)
AB_2832276 (BioLegend Cat. No. 101419)
AB_2832276 (BioLegend Cat. No. 101420)
AB_2832276 (BioLegend Cat. No. 101421)
AB_2832276 (BioLegend Cat. No. 101422)

Antigen Details

Structure
Integrin family, associates with CD11a, CD11b, CD11c, 95 kD
Distribution

All leukocytes

Function
Adhesion
Ligand/Receptor
ICAM-1 (CD54), ICAM-2 (CD102), ICAM-3 (CD50), iC3b, fibrinogen
Cell Type
Leukocytes, Tregs
Biology Area
Cell Adhesion, Cell Biology, Immunology, Innate Immunity
Molecular Family
Adhesion Molecules, CD Molecules
Antigen References

1. Barclay AN, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Anderson DC, et al. 1987. Annu. Rev. Med. 38:175.
3. Wilson RW, et al. 1993. J. Immunol. 151:1571.

Gene ID
16414 View all products for this Gene ID
UniProt
View information about CD18 on UniProt.org
Go To Top Version: 1    Revision Date: 02/28/2020

8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

Login/Register
Forgot your password? Reset Password
Request an Account