- Clone
- QA18A73 (See other available formats)
- Regulatory Status
- RUO
- Other Names
- Ms4a1, Ly-44
- Isotype
- Mouse IgG2a, κ
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C57BL/6 mouse splenocytes were stained with anti-mouse/human CD45R/B220 APC and purified anti-mouse CD20 recombinant (clone QA18A73) (left) or purified mouse IgG2a, κ isotype control (right), followed by PE anti-mouse IgG2a. -
C57BL/6 mice were retro-orbitally injected with 200 µg of Ultra-LEAF™ purified anti-mouse CD20 (clone SA271G2, black line, N=4), Ultra-LEAF™ purified anti-mouse CD20 recombinant (clone QA18A73, purple line, N=4), or vehicle only (PBS, red line, N=4). Peripheral blood was analyzed at indicated intervals, and average % B cells calculated for each group (measured as % CD19+B220+ cells).
| Cat # | Size | Price | Quantity Check Availability | ||
|---|---|---|---|---|---|
| 161407 | 100 µg | $94.00 | |||
| 161408 | 1 mg | $149.00 | |||
Select size of product is eligible for a 40% discount! Promotion valid until December 31, 2024. Exclusions apply. To view full promotion terms and conditions or to contact your local BioLegend representative to receive a quote, visit our webpage.
CD20 is a 33-37 kD protein, a member of the MS4A family, with four transmembrane spanning regions that present as a homo-oligomeric complexes in the cell surface when associating with MHC class I and II, CD53, CD81, and CD82. CD20 is expressed on B cells and a subset of T cells, but not on plasma cells. CD20 regulates B-cell activation and proliferation. Its ligation promotes transmembrane Ca2+ trafficking. CD20 is an important therapeutic target in the treatment of B cell lymphomas and leukemias.
Product Details
- Verified Reactivity
- Mouse
- Antibody Type
- Recombinant
- Host Species
- Mouse
- Immunogen
- Mouse CD20 transfected cells
- Formulation
- 0.2 µm filtered in phosphate-buffered solution, pH 7.2, containing no preservative.
- Preparation
- The Ultra-LEAF™ (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.
- Concentration
- The antibody is bottled at the concentration indicated on the vial, typically between 2 mg/mL and 3 mg/mL. To obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C. This Ultra-LEAF™ solution contains no preservative; handle under aseptic conditions.
- Application
-
FC - Quality tested
Depletion - Verified - Recommended Usage
-
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.5 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
-
This recombinant antibody clone is derived from clone SA271G2.
- Additional Product Notes
-
A single i.v. dose of 200 µg of mAb clone QA18A73 depletes B cells from peripheral blood for more than 30 days.
- RRID
-
AB_2910341 (BioLegend Cat. No. 161407)
AB_2910341 (BioLegend Cat. No. 161408)
Antigen Details
- Structure
- Member of the MS4A family, four transmembrane spanning regions, three isoforms of 37, 35, and 37 kD, depending on the phosphorylation degree.
- Distribution
-
B cells and a subset of T cells
- Function
- Regulates B-cell activation and proliferation
- Interaction
- Associates with MHC class I and II, CD53, CD81, and CD82
- Antigen References
-
1. Morsy DE, et al. 2013. J. Immunol. 191:3112.
2. Pang X, et al. 2017. Hum Gene Ther. 28(2):216-225.
3. Uchida J, et al. 2004. Int Immunol. 16(1):119-29 - Gene ID
- 12482 View all products for this Gene ID
- UniProt
- View information about CD20 on UniProt.org
Other Formats
View All CD20 Reagents Request Custom Conjugation| Description | Clone | Applications |
|---|---|---|
| Purified anti-mouse CD20 Recombinant Antibody | QA18A73 | FC |
| APC anti-mouse CD20 Recombinant Antibody | QA18A73 | FC |
| PE anti-mouse CD20 Recombinant Antibody | QA18A73 | FC |
| Ultra-LEAF™ Purified anti-mouse CD20 Recombinant Antibody | QA18A73 | FC,Depletion |
| FITC anti-mouse CD20 Recombinant Antibody | QA18A73 | FC |
Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
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Purified anti-mouse CD20 Recombinant Antibody
C57BL/6 mouse splenocytes were stained with anti-mouse/human... -
APC anti-mouse CD20 Recombinant Antibody
C57BL/6 mouse splenocytes were stained with anti-mouse/human... -
PE anti-mouse CD20 Recombinant Antibody
C57BL/6 mouse splenocytes were stained with anti-mouse/human... -
Ultra-LEAF™ Purified anti-mouse CD20 Recombinant Antibody
C57BL/6 mouse splenocytes were stained with anti-mouse/human... 
C57BL/6 mice were retro-orbitally injected with 200 µg of Ul... -
FITC anti-mouse CD20 Recombinant Antibody
C57BL/6 mouse splenocytes were stained with anti-mouse/human...