- Clone
- A18246G (See other available formats)
- Regulatory Status
- RUO
- Other Names
- IL-2Rα, Ly-43, p55, Tac
- Isotype
- Rat IgG2b, κ
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250 µg of Ultra-LEAF™ Purified anti-mouse CD25 antibody (clone A18246G) (left) or 250 µg Ultra-LEAF™ Purified rat IgG2b, κ isotype control (clone RTK4530) (right) were intraperitoneally (IP) injected to C57BL/6 mice, and splenocytes analyzed on day 7 post-injection. Cells shown are gated CD3+ splenocyte population. -
% CD3+CD4+CD25+ splenocytes analyzed in mice intraperitoneally injected with 250 µg Ultra-LEAF™ Rat IgG2b, κ Isotype Ctrl (clone RTK4530) (black line) or Ultra-LEAF™ anti-mouse CD25 (clone A18246G) (purple line) at indicated days post-injection. Error bars represent standard deviation from 3 mice analyzed per condition and timepoint.
| Cat # | Size | Price | Quantity Check Availability | ||
|---|---|---|---|---|---|
| 112603 | 100 µg | $209.00 | |||
| 112604 | 1 mg | $600.00 | |||
Select size of product is eligible for a 40% discount! Promotion valid until December 31, 2024. Exclusions apply. To view full promotion terms and conditions or to contact your local BioLegend representative to receive a quote, visit our webpage.
CD25, also known as the low affinity IL-2Rα, Ly-43, p55, or Tac, is a 55 kD glycoprotein. It is expressed on activated T and B cells, thymocyte subsets, pre-B cells, and T regulatory cells. In association with CD122 (IL-2Rβ) and CD132 (common γ chain), CD25 forms the high affinity signaling IL-2 receptor. CD25 plays a role in lymphocyte differentiation and activation/proliferation.
Product Details
- Verified Reactivity
- Mouse
- Antibody Type
- Monoclonal
- Host Species
- Rat
- Formulation
- 0.2 µm filtered in phosphate-buffered solution, pH 7.2, containing no preservative.
- Preparation
- The Ultra-LEAF™ (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.
- Concentration
- The antibody is bottled at the concentration indicated on the vial, typically between 2 mg/mL and 3 mg/mL. To obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C. This Ultra-LEAF™ solution contains no preservative; handle under aseptic conditions.
- Application
-
FC - Quality tested
Depletion - Verified - Recommended Usage
-
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.5 µg per million cells in 100 µL volume. For in vivo depletion, suggested use is 250 µg of Ultra-LEAF™ purified of clone A18246G for one C57BL/6 mouse intraperitoneal injection. It is recommended that the reagent be titrated for optimal performance.
- Additional Product Notes
-
Ultra-LEAF™ purified (clone A18246G) antibody recognizes a different epitope compared to other mouse CD25 such as clones 3C7 and PC61.
Ultra-LEAF™ purified (clone A18246G) antibody can deplete CD25+ cells in vivo in C57BL/6 mouse by intraperitoneal injection. - RRID
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AB_2941391 (BioLegend Cat. No. 112603)
AB_2941391 (BioLegend Cat. No. 112604)
Antigen Details
- Structure
- Forms high affinity IL-2R with IL-2Rβ (CD122) and IL-2Rγ (CD132), 55 kD
- Distribution
-
Activated T cells and B cells, thymocyte subset, pre-B cells, T regulatory cells
- Function
- IL-2 receptor
- Ligand/Receptor
- IL-2
- Cell Type
- B cells, T cells, Tregs
- Biology Area
- Immunology
- Molecular Family
- CD Molecules, Cytokine/Chemokine Receptors
- Antigen References
-
- Taniguchi T, et al. 1993. Cell. 73:5-8.
- Waldmann TA. 1991. J Biol Chem. 266:2681-4.
- Read S, et al. 2000. J Exp Med. 192:295-302.
- Lowenthal JW, et al. 1985. J Immunol. 135:3988-94.
- Gene ID
- 16184 View all products for this Gene ID
- UniProt
- View information about CD25 on UniProt.org
Other Formats
View All CD25 Reagents Request Custom Conjugation| Description | Clone | Applications |
|---|---|---|
| Ultra-LEAF™ Purified anti-mouse CD25 | A18246G | FC,IP |
| Spark PLUS UV395™ anti-mouse CD25 | A18246G | FC |
Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
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Ultra-LEAF™ Purified anti-mouse CD25
250 µg of Ultra-LEAF™ Purified anti-mouse CD25 antibody (clo... 
% CD3+CD4+CD25+ splenocytes analyzed in mice intraperitoneal... -
Spark PLUS UV395™ anti-mouse CD25
C57BL/6 mouse splenocytes were stained with anti-mouse CD4 (...