- Clone
- IK22/5 (See other available formats)
- Regulatory Status
- RUO
- Other Names
- Tumor necrosis factor-related activation-induced cytokine, Receptor activator of NF-κB ligand (RANK L), Osteoprotegerin ligand (OPGL), Osteoclast differentiation factor (ODF), SOFA, TNFSF-11, CD254, RANKL
- Isotype
- Rat IgG2a, κ
-
Mouse TRANCE transfected cells stained with Ultra-LEAF™ Purified IK22-5, followed by biotinylated anti-rat IgG and Sav-PE
| Cat # | Size | Price | Quantity Check Availability | ||
|---|---|---|---|---|---|
| 510009 | 100 µg | $110.00 | |||
| 510010 | 1 mg | $149.00 | |||
| 510011 | 5 mg | $539.00 | |||
| 510012 | 25 mg | $1408.00 | |||
| 510013 | 50 mg | $2354.00 | |||
| 510014 | 100 mg | $3383.00 | |||
Select size of product is eligible for a 40% discount! Promotion valid until December 31, 2024. Exclusions apply. To view full promotion terms and conditions or to contact your local BioLegend representative to receive a quote, visit our webpage.
Product Details
- Verified Reactivity
- Mouse
- Antibody Type
- Monoclonal
- Host Species
- Rat
- Immunogen
- NSO-derived recombinant mouse TRANCE
- Formulation
- 0.2 µm filtered in phosphate-buffered solution, pH 7.2, containing no preservative.
- Preparation
- The Ultra-LEAF™ (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.
- Concentration
- The antibody is bottled at the concentration indicated on the vial, typically between 2 mg/mL and 3 mg/mL. Older lots may have also been bottled at 1 mg/mL. To obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C. This Ultra-LEAF™ solution contains no preservative; handle under aseptic conditions.
- Application
-
FC - Quality tested
IP, WB, Block - Reported in the literature, not verified in house - Recommended Usage
-
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
-
The IK22/5 antibody has been reported to block the binding of RANK ligand-Fc to RANK. Additional reported applications (for the relevant formats) include: immunoprecipitation2, Western blotting2, and blocking of ligand binding1,2,4. The Ultra-LEAF™ purified antibody (Endotoxin <0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 510009-510014).
-
Application References
(PubMed link indicates BioLegend citation) -
- Miyahira Y, et al. 2003. J. Immunol. 171:6344. (Block)
- Personal communication (Block IP WB)
- Gao Y, et al. 2007. J. Clin. Invest. 117:122.
- Kamijo S, et al. 2006. Biochem. Biophys. Res. Commun. 347:124. (Block)
- Fionda C, et al. 2007. J. Immunol. 178:4039.
- Haslam SZ, et al. 2008. Endocrinology. 149:2098. (Block) PubMed
- RRID
-
AB_2832814 (BioLegend Cat. No. 510009)
AB_2832814 (BioLegend Cat. No. 510010)
AB_2832814 (BioLegend Cat. No. 510011)
AB_2832814 (BioLegend Cat. No. 510012)
AB_2832814 (BioLegend Cat. No. 510013)
AB_2832814 (BioLegend Cat. No. 510014)
Antigen Details
- Structure
- TNF superfamily; 19 kD (Mammalian)
- Distribution
-
Activated T cells, osteoclasts
- Function
- Regulates T and dendritic cell interactions, and osteoclast differentiation
- Ligand/Receptor
- RANK, OPG
- Bioactivity
- Activates JNK and NF-κB; involved in bone resorption; induces the expression of IL-1, IL-6, IL-12, IL-15 in dendritic cells
- Cell Sources
- Activated T cells, myeloid bone marrow precursors, stromal fibroblasts, synovial cells, osteoblasts
- Cell Targets
- Dendritic cells, osteoclasts
- Receptors
- ODFR, ODAR, TRANCE-R
- Cell Type
- Osteoclasts, T cells
- Biology Area
- Cell Biology, Immunology
- Molecular Family
- CD Molecules, Cytokines/Chemokines
- Antigen References
-
1. Fitzgerald K, et al. Eds. 2001. The Cytokine FactsBook. Academic Press San Diego.
2. Josien R, et al. 1999. J. Immunol. 162:2562.
3. Takayanagi H, et al. 2000. Nature 408:600.
4. Wong B, et al. 1999. Mol. Cell. 4:1041. - Regulation
- Type II integral membrane protein processed by TACE for secretion; upregulated upon TCR engagement
- Gene ID
- 21943 View all products for this Gene ID
- UniProt
- View information about CD254 on UniProt.org
Other Formats
View All CD254 Reagents Request Custom Conjugation| Description | Clone | Applications |
|---|---|---|
| Biotin anti-mouse CD254 (TRANCE, RANKL) | IK22/5 | FC |
| PE anti-mouse CD254 (TRANCE, RANKL) | IK22/5 | FC |
| Purified anti-mouse CD254 (TRANCE, RANKL) | IK22/5 | FC,IP,WB |
| Ultra-LEAF™ Purified anti-mouse CD254 (TRANCE, RANKL) | IK22/5 | FC,IP,WB,Block |
Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
-
Biotin anti-mouse CD254 (TRANCE, RANKL)
Mouse TRANCE transfected cells stained with biotinylated IK2... -
PE anti-mouse CD254 (TRANCE, RANKL)
TRANCE transfected cells stained with IK22-5 PE -
Purified anti-mouse CD254 (TRANCE, RANKL)
Mouse TRANCE transfected cells stained with purified IK22-5,... -
Ultra-LEAF™ Purified anti-mouse CD254 (TRANCE, RANKL)
Mouse TRANCE transfected cells stained with Ultra-LEAF™ Puri...