- Clone
- A18238E (See other available formats)
- Regulatory Status
- RUO
- Other Names
- CD272, B- and T-lymphocyte attenuator, B- and T-lymphocyte-associated protein
- Isotype
- Rat IgG2a, κ
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Recombinant mouse HVEM-Fc chimera (Cat. No. 771306) (black circles) binds to immobilized recombinant mouse BTLA-Fc chimera (Cat. No. 775806). Ultra-LEAF™ purified anti-mouse CD272 (BTLA) antibody (clone A18238E) (purple squares) inhibits the binding in a dose-dependent manner whereas the GoInViv™ purified rat IgG2a, κ isotype ctrl antibody (Cat. No. 400562) (gray triangles) does not have the effect. Ultra-LEAF™ purified anti-mouse CD272 (BTLA) antibody blocks the binding of 0.03 µg/mL recombinant mouse HVEM-Fc to 1 µg/mL immobilized recombinant mouse BTLA-Fc chimera. ND50 = 0.08 – 0.5 µg/mL.
| Cat # | Size | Price | Quantity Check Availability | ||
|---|---|---|---|---|---|
| 941803 | 100 µg | $336.00 | |||
| 941804 | 1 mg | $1045.00 | |||
Select size of product is eligible for a 40% discount! Promotion valid until December 31, 2024. Exclusions apply. To view full promotion terms and conditions or to contact your local BioLegend representative to receive a quote, visit our webpage.
BTLA (B and T lymphocyte attenuator, CD272) is a type I transmembrane glycoprotein with four extracellular cysteine-rich domains. It is an inhibitory molecule structurally and functionally similar to cytotoxic T lymphocyte antigen-4 (CTLA-4) and programmed death-1 (PD-1). These molecules belong to the immunoglobulin superfamily that binds B7 family members. Different from CTLA-4 and PD-1 binding with the B7 family members, BTLA interacts with herpes virus entry mediator (HVEM) which belongs to TNFR family. BTLA/HVEM interaction was the first demonstration of crosstalk between these two family members. BTLA/HVEM interaction negatively modulates T cell activation. HVEM induces BTLA tyrosine phosphorylation and inhibits T-cell proliferation. There is accumulating evidence suggesting that BTLA/HVEM signaling also plays an important role in autoimmunity and infection at mucosal surfaces. The BTLA/HVEM interaction does not prevent the simultaneous binding of other HVEM ligands such as LIGHT or Lymphotoxin-alpha. BTLA is expressed on T cells, B cells, macrophages, dendritic cells, and natural killer cells. Constitutive expression level of BTLA on resting T cells are very low, but increase after the activation of T cells. BTLA is highly expressed on resting B cells.
Product Details
- Verified Reactivity
- Mouse
- Antibody Type
- Monoclonal
- Host Species
- Rat
- Immunogen
- Recombinant mouse CD272 (BTLA)
- Formulation
- 0.2 µm filtered in phosphate-buffered solution, pH 7.2, containing no preservative.
- Preparation
- The Ultra-LEAF™ (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.
- Concentration
- The antibody is bottled at the concentration indicated on the vial, typically between 2 mg/mL and 3 mg/mL. Older lots may have also been bottled at 1 mg/mL. To obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C. This Ultra-LEAF™ solution contains no preservative; handle under aseptic conditions.
- Application
-
Block - Quality tested
- Recommended Usage
-
Each lot of this antibody is quality control tested by blocking the binding between mouse BTLA-Fc chimera and mouse HVEM-Fc chimera. Ultra-LEAFTM purified anti-mouse CD272 (BTLA) antibody blocks the binding of 0.03 µg/mL recombinant mouse HVEM-Fc chimera to 1 µg/mL immobilized recombinant mouse BTLA-Fc chimera. ND50 range: 0.08 – 0.5 µg/mL. It is recommended that the reagent be titrated for optimal performance for each application.
- RRID
-
AB_2904452 (BioLegend Cat. No. 941803)
AB_2904452 (BioLegend Cat. No. 941804)
Antigen Details
- Structure
- An Ig superfamily co-inhibitory receptor with structural similarity to programmed cell death 1 (PD-1) and CTLA-4
- Distribution
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Lymphocytes, T cells, B cells, macrophages, dendritic cells, NKT cells and natural killer cells.
- Function
- Negative regulator of T cell activation and proliferation, attenuate B cell proliferation upon associating with its known ligand, herpes virus entry mediator (HVEM)
- Interaction
- Lymphocytes
- Ligand/Receptor
- HVEM
- Cell Type
- B cells, Dendritic cells, Lymphocytes, Macrophages, NK cells, NKT cells, T cells
- Biology Area
- Adaptive Immunity, Immunology, Innate Immunity
- Molecular Family
- Immune Checkpoint Receptors
- Antigen References
-
- Watanabe N, et al. 2003. Nat. Immunol. 4: 670-9
- Gavrieli M, et al. 2003. Biochem. Biophys. Res. Commun. 312: 1236-43
- Gonzalez LC, et al. 2005. Proc. Natl. Acad. Sci. 102: 1116-21
- Sedy JR, et al. 2005. Nat. Immunol. 6: 90-8
- Murphy KM, et al. 2006. Nat. Rev. Immunol. 6: 671-81
- Gene ID
- 208154 View all products for this Gene ID
- UniProt
- View information about CD272 on UniProt.org
Other Formats
View All CD272 Reagents Request Custom Conjugation| Description | Clone | Applications |
|---|---|---|
| Ultra-LEAF™ Purified anti-mouse CD272 (BTLA) Antibody | A18238E | Block |
Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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Ultra-LEAF™ Purified anti-mouse CD272 (BTLA) Antibody
Recombinant mouse HVEM-Fc chimera (Cat. No. 771306) (black c...