- Clone
- FGK45 (See other available formats)
- Regulatory Status
- RUO
- Other Names
- Bp50, TNFRSF5
- Isotype
- Rat IgG2a, κ
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C57BL/6 mouse Splenocytes were stained with Ultra-LEAF™ CD40 (clone FGK45) (left) or Ultra-LEAF™ rat IgG2a, κ isotype (right) followed by FITC anti-rat IgG and APC CD3.
| Cat # | Size | Price | Quantity Check Availability | ||
|---|---|---|---|---|---|
| 157503 | 100 µg | $215.00 | |||
| 157504 | 1 mg | $622.00 | |||
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CD40 is a 48 kD type I transmembrane glycoprotein also known as Bp50. It is a member of the tumor necrosis factor receptor (TNFR) superfamily and is expressed on B cells, basal epithelial cells, macrophages, follicular dendritic cells, endothelial cells, and a subset of CD34+ hematopoietic progenitors. CD40 regulates B cell development/maturation, Ig isotype switching and, in combination with other signals such as IL-4, protects B cells from surface Ig-induced apoptosis and promotes proliferation. Interaction of CD40 with its ligand CD154 (gp39), which is expressed on activated T cells, is important in costimulation and immune regulation.
Product Details
- Verified Reactivity
- Mouse
- Antibody Type
- Monoclonal
- Host Species
- Rat
- Immunogen
- Recombinant mouse CD40 – Fc fusion protein
- Formulation
- 0.2 µm filtered in phosphate-buffered solution, pH 7.2, containing no preservative.
- Preparation
- The Ultra-LEAF™ (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.
- Concentration
- The antibody is bottled at the concentration indicated on the vial, typically between 2 mg/mL and 3 mg/mL. Older lots may have also been bottled at 1 mg/mL. To obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C. This Ultra-LEAF™ solution contains no preservative; handle under aseptic conditions.
- Application
-
FC - Quality tested
Activ - Verified - Recommended Usage
-
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 2.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
-
The FGK45 antibody can block the binding of HM40-3 clone. The FGK45 antibody can also stimulate mouse B cell proliferation.
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Application References
(PubMed link indicates BioLegend citation) -
- Andersson J, et al. 1996. Immunity 5:319-330.
- Melief CJ, et al. 1998. Nature 393(6684):480-3.
- Londei M, et al. 2000. Nat Med 6(6):673-9.
- Tominaga T, et al. 2015. Neuro Oncol 17(11):1453-62.
- RRID
-
AB_2814090 (BioLegend Cat. No. 157503)
AB_2814090 (BioLegend Cat. No. 157504)
Antigen Details
- Structure
- 48 kD type I transmembrane glycoprotein, a member of the tumor necrosis factor receptor (TNFR) superfamily.
- Distribution
-
Expressed on B lymphocytes and other antigen-presenting cells, such as macrophages, follicular dendritic cells, etc.
- Function
- Regulates B cell growth and differentiation and Ig isotype switching. Interaction of CD40 with its ligand, CD154, on activated T cells is important for immune response.
- Interaction
- TRAF molecules
- Ligand/Receptor
- CD154 (CD40L)
- Cell Type
- Antigen-presenting cells, B cells, Dendritic cells, Macrophages
- Biology Area
- Cell Biology, Costimulatory Molecules, Immunology, Neuroscience, Neuroscience Cell Markers
- Molecular Family
- CD Molecules
- Antigen References
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- Barclay A, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
- Bancherou J, et al. 1994. Annu. Rev. Immunol. 12:881.
- Clark EA, et al. 1996. P. Natl. Acad. Sci. USA 83:4494.
- Grewal IS, et al. 1998. Annu Rev Immunol 16:111.
- Gene ID
- 21939 View all products for this Gene ID
- UniProt
- View information about CD40 on UniProt.org
Other Formats
View All CD40 Reagents Request Custom Conjugation| Description | Clone | Applications |
|---|---|---|
| Ultra-LEAF™ Purified anti-mouse CD40 | FGK45 | FC,Activ |
| PE anti-mouse CD40 | FGK45 | FC |
Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
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Ultra-LEAF™ Purified anti-mouse CD40
C57BL/6 mouse Splenocytes were stained with Ultra-LEAF™ CD40... -
PE anti-mouse CD40
C57BL/6 mouse splenocytes were stained with APC CD3 and PE C...