- Clone
- HM40-3 (See other available formats)
- Regulatory Status
- RUO
- Other Names
- Bp50, TNFRSF5
- Isotype
- Armenian Hamster IgM
-
C57BL/6 mouse splenocytes stained with CD3ε APC and CD40 (clone HM40-3) purified (left) or Armenian Hamster IgM isotype control (right) followed by Biotin anti-Armenian Hamster IgG and Streptavidin-PE.
| Cat # | Size | Price | Quantity Check Availability | ||
|---|---|---|---|---|---|
| 102913 | 100 µg | $176.00 | |||
| 102914 | 1 mg | $545.00 | |||
Select size of product is eligible for a 40% discount! Promotion valid until December 31, 2024. Exclusions apply. To view full promotion terms and conditions or to contact your local BioLegend representative to receive a quote, visit our webpage.
CD40 is a 48 kD type I transmembrane glycoprotein, also known as Bp50. It is a member of the tumor necrosis factor receptor (TNFR) superfamily, expressed on B cells, basal epithelial cells, macrophages, follicular dendritic cells, endothelial cells, and a subset of CD34+ hematopoietic progenitors. CD40 regulates B cell development/maturation, Ig isotype switching and, in combination with other signals such as IL-4, protects B cells from surface Ig-induced apoptosis and promotes proliferation. Interaction of CD40 with its ligand CD154 (gp39) expressed on activated T cells, is important in costimulation and immune regulation. The HM40-3 antibody has been reported to stimulate dendritic and B cell proliferation and block CD40-CD40L interactions.
Product Details
- Verified Reactivity
- Mouse
- Antibody Type
- Monoclonal
- Host Species
- Armenian Hamster
- Immunogen
- (BALB/c x NZB)F1 mouse-derived lymphoma WEHI 231
- Formulation
- 0.2 µm filtered in phosphate-buffered solution, pH 7.2, containing no preservative.
- Preparation
- The Ultra-LEAF™ (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.
- Concentration
- The antibody is bottled at the concentration indicated on the vial, typically between 2 mg/mL and 3 mg/mL. Older lots may have also been bottled at 1 mg/mL. To obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C. This Ultra-LEAF™ solution contains no preservative; handle under aseptic conditions.
- Application
-
FC - Quality tested
Block, Costim, IHC-F - Reported in the literature, not verified in house - Recommended Usage
-
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 1.0 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
-
Additional reported applications (for the relevant formats) include: blocking of CD40 ligand binding1, stimulation of mouse B cell and dendritic cell proliferation2-6,9, and immunohistochemistry7 of acetone-fixed frozen sections. The Ultra-LEAF™ purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. Nos. 102913 & 102914).
-
Application References
(PubMed link indicates BioLegend citation) -
- Kawano T, et al. 1997. Science 278:1626. (Block)
- Kaneko Y, et al. 1996. Eur. J. Immunol. 26:3061. (Costim)
- Kashiwada M, et al. 1996. Eur. J. Immunol. 26:1451. (Costim)
- Inaba M, et al. 1995. Eur. J. Immunol. 25:1244. (Costim)
- Akiba H, et al. 1999. J. Immunol. 162:7058. (Costim)
- Ridge JP, et al. 1998. Nature 393:474. (Costim)
- Morelli AE, et al. 2000. Transplantation 69:2647. (IHC)
- Ma XT, et al. 2006. Cancer Research 66:1169.
- Yu X, et al. 2006. J. Biol. Chem. 281:15505. (Costim)
- del Rio ML, et al. 2011. Transpl. Int. 24:501. (FC) PubMed
- RRID
-
AB_2819787 (BioLegend Cat. No. 102913)
AB_2819787 (BioLegend Cat. No. 102914)
Antigen Details
- Structure
- TNFR superfamily, 48 kD
- Distribution
-
B cells, basal epithelial cells, macrophages, follicular dendritic cells, endothelial cells, CD34+ hematopoietic progenitors
- Function
- Stimulates B cell growth, differentiation and isotype switching, stimulates macrophage and dendritic cell cytokine production
- Ligand/Receptor
- CD40L (CD154)
- Cell Type
- B cells, Dendritic cells, Endothelial cells, Epithelial cells, Hematopoietic stem and progenitors, Macrophages
- Biology Area
- Cell Biology, Costimulatory Molecules, Immunology, Neuroscience, Neuroscience Cell Markers
- Molecular Family
- CD Molecules
- Antigen References
-
1. Barclay A, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Bancherou J, et al. 1994. Annu. Rev. Immunol. 12:881.
3. Clark EA, et al. 1996. P. Natl. Acad. Sci. USA 83:4494. - Gene ID
- 21939 View all products for this Gene ID
- UniProt
- View information about CD40 on UniProt.org
Other Formats
View All CD40 Reagents Request Custom Conjugation| Description | Clone | Applications |
|---|---|---|
| FITC anti-mouse CD40 | HM40-3 | FC |
| Purified anti-mouse CD40 | HM40-3 | FC,Block,Costim,IHC-F |
| Alexa Fluor® 488 anti-mouse CD40 | HM40-3 | FC |
| Alexa Fluor® 647 anti-mouse CD40 | HM40-3 | FC |
| Ultra-LEAF™ Purified anti-mouse CD40 | HM40-3 | FC,Block,Costim,IHC-F |
Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
-
FITC anti-mouse CD40
C57BL/6 mouse splenocytes stained with HM40-3 FITC -
Purified anti-mouse CD40
C57BL/6 mouse splenocytes stained with CD3ε APC and ... -
Alexa Fluor® 488 anti-mouse CD40
C57BL/6 mouse splenocytes stained with HM40-3 Alexa Fluor® 4... -
Alexa Fluor® 647 anti-mouse CD40
C57BL/6 mouse splenocytes stained with HM40-3 Alexa Fluor® 6... -
Ultra-LEAF™ Purified anti-mouse CD40
C57BL/6 mouse splenocytes stained with CD3ε APC and CD40 (cl...