- Clone
- S20005A (See other available formats)
- Regulatory Status
- RUO
- Other Names
- CAMPATH-1 antigen
- Isotype
- Rat IgG2a, κ
-
C57BL/6 mouse splenocytes were stained with anti-mouse CD3 APC and Ultra-LEAF™ purified anti-mouse CD52 (clone S20005A) (left) or Ultra-LEAF™ purified rat Ig2a, κ isotype control (right) followed by anti-rat IgG PE.
| Cat # | Size | Price | Quantity Check Availability | ||
|---|---|---|---|---|---|
| 165703 | 100 µg | $270.00 | |||
| 165704 | 1 mg | $743.00 | |||
Select size of product is eligible for a 40% discount! Promotion valid until December 31, 2024. Exclusions apply. To view full promotion terms and conditions or to contact your local BioLegend representative to receive a quote, visit our webpage.
CAMPATH-1 antigen, also known as cluster of differentiation 52 (CD52), is a glycoprotein that in humans is encoded by the CD52 gene. It is expressed in the embryo mesenchyme and thymus primordium. CD52 acts upstream of or within response to bacterium and is located in the sperm midpiece. CD52 is present on the surface of mature lymphocytes, but not on the stem cells from which these lymphocytes were derived. It also is found on monocytes and dendritic cells. Furthermore, it is found within the male genital tract and is present on the surface of mature sperm cells. Due to its highly negative charge and presence on sperm cells and lymphocytes, it has been thought that CD52 functions as an anti-adhesion since it allows cells to freely move around.
Product Details
- Verified Reactivity
- Mouse
- Antibody Type
- Monoclonal
- Host Species
- Rat
- Formulation
- 0.2 µm filtered in phosphate-buffered solution, pH 7.2, containing no preservative.
- Preparation
- The Ultra-LEAF™ (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.
- Concentration
- The antibody is bottled at the concentration indicated on the vial, typically between 2 mg/mL and 3 mg/mL. To obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C. This Ultra-LEAF™ solution contains no preservative; handle under aseptic conditions.
- Application
-
FC - Quality tested
- Recommended Usage
-
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.
- RRID
-
AB_2941444 (BioLegend Cat. No. 165703)
AB_2941444 (BioLegend Cat. No. 165704)
Antigen Details
- Structure
- CD52 is a peptide of 12 amino acids, anchored to glycosylphosphatidylinositol (GPI).
- Distribution
-
Expressed on lymphohematopoietic tissues, including thymus, spleen, and bone marrow, but not in liver, kidney, and brain.
- Function
- Promote cell-mediated killing (antibody-dependent cellular cytotoxicity)
- Cell Type
- B cells, Eosinophils, Erythrocytes, Macrophages, Monocytes, T cells
- Biology Area
- Costimulatory Molecules, Immunology
- Molecular Family
- CD Molecules
- Antigen References
-
- Gudrun R, et al. 2002. Blood. 101:1422.
- Gene ID
- 23833 View all products for this Gene ID
- UniProt
- View information about CD52 on UniProt.org
Other Formats
View All CD52 Reagents Request Custom Conjugation| Description | Clone | Applications |
|---|---|---|
| Ultra-LEAF™ Purified anti-mouse CD52 | S20005A | FC |
| PE anti-mouse CD52 | S20005A | FC |
Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
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Ultra-LEAF™ Purified anti-mouse CD52
C57BL/6 mouse splenocytes were stained with anti-mouse CD3 A... -
PE anti-mouse CD52
C57BL/6 mouse splenocytes were stained with anti-mouse CD3 (...