- Clone
- H22 (See other available formats)
- Regulatory Status
- RUO
- Other Names
- Interferon-γ, Immune interferon, Type II interferon, T cell interferon, Macrophage-activating factor (MAF)
- Isotype
- Armenian Hamster IgG
-
Recombinant Mouse IFN-γ was resolved by electrophoresis, transferred to nitrocellulose, and probed with anti-mouse IFN-γ antibody (clone H22). Proteins were visualized using a goat anti-Armenian hamster IgG secondary conjugated to HRP and chemiluminescence detection.
| Cat # | Size | Price | Quantity Check Availability | ||
|---|---|---|---|---|---|
| 513207 | 100 µg | $110.00 | |||
| 513208 | 1 mg | $149.00 | |||
| 513209 | 5 mg | $539.00 | |||
| 513210 | 25 mg | $1408.00 | |||
| 513211 | 50 mg | $2354.00 | |||
| 513212 | 100 mg | $3383.00 | |||
Select size of product is eligible for a 40% discount! Promotion valid until December 31, 2024. Exclusions apply. To view full promotion terms and conditions or to contact your local BioLegend representative to receive a quote, visit our webpage.
Interferon-γ is a potent multifunctional cytokine which is secreted primarily by activated NK cells and T cells. Originally characterized based on anti-viral activities, IFN-γ also exerts anti-proliferative, immunoregulatory, and proinflammatory activities. IFN-γ can upregulate MHC class I and II antigen expression by antigen-presenting cells.
Product Details
- Verified Reactivity
- Mouse
- Antibody Type
- Monoclonal
- Host Species
- Armenian Hamster
- Immunogen
- Recombinant full-length murine IFN-γ
- Formulation
- 0.2 µm filtered in phosphate-buffered solution, pH 7.2, containing no preservative.
- Preparation
- The Ultra-LEAF™ (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.
- Concentration
- The antibody is bottled at the concentration indicated on the vial, typically between 2 mg/mL and 3 mg/mL. Older lots may have also been bottled at 1 mg/mL. To obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C. This Ultra-LEAF™ solution contains no preservative; handle under aseptic conditions.
- Application
-
WB - Quality tested
Neut, ELISA, ICC, IP - Reported in the literature, not verified in house - Recommended Usage
-
Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.5 µg per mL. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
-
The purified H22 antibody is useful in mouse IFN-γ ELISA, immunofluorescence and immunoprecipitation applications. For in vitro or in vivo neutralization applications, it is suggested to use LEAF™ Purified H22. It is recommended that the reagent be titrated for optimal performance for each application.
-
Application References
(PubMed link indicates BioLegend citation) -
- Schreiber RD, et al. 1985. J. Immunol. 134:1609.
- Williams RO, et al. 1993. Clin. Exp. Immunol. 92:323
- Steed AL, et al. 2006. J. Virol. 80:192.
- Wu LL, et al. 2014. Am J Pathol. 184:2260. PubMed
- Product Citations
-
- RRID
-
AB_2810645 (BioLegend Cat. No. 513207)
AB_2810645 (BioLegend Cat. No. 513208)
AB_2810645 (BioLegend Cat. No. 513209)
AB_2810645 (BioLegend Cat. No. 513210)
AB_2810645 (BioLegend Cat. No. 513211)
AB_2810645 (BioLegend Cat. No. 513212)
Antigen Details
- Structure
- Cytokine; dimer; 40-80 kD (Mammalian)
- Function
- Upregulated by IL-2, FGF-basic, EGF; downregulated by 1-α-25-Dihydroxy vitamin D3, dexamethasone
- Interaction
- T cells, B cells, macrophages, NK cells, endothelial cells, fibroblasts
- Ligand/Receptor
- IFN-γRα (CDw119) dimerized with IFN-γRβ (AF-1)
- Bioactivity
- Antiviral/antiparasitic activities; inhibits proliferation; enhances MHC class I and II expression on APC
- Cell Sources
- CD8+ and CD4+ T cells, NK cells
- Biology Area
- Immunology
- Molecular Family
- Cytokines/Chemokines
- Antigen References
-
1. Fitzgerald K, et al. Eds. 2001. The Cytokine FactsBook. Academic Press San Diego.
2. De Maeyer E, et al. 1992. Curr. Opin. Immunol. 4:321
3. Farrar M, et al. 1993. Annu. Rev. Immunol. 11:571
4. Gray P, et al. 1987. Lymphokines 13:151 - Gene ID
- 15978 View all products for this Gene ID
- UniProt
- View information about IFN-gamma on UniProt.org
Other Formats
View All IFN-γ Reagents Request Custom Conjugation| Description | Clone | Applications |
|---|---|---|
| Purified anti-mouse IFN-γ | H22 | WB,ELISA,ICC,IP |
| Ultra-LEAF™ Purified anti-mouse IFN-γ | H22 | WB,Neut,ELISA,ICC,IP |
Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
-
Purified anti-mouse IFN-γ
Western blot analysis of 50 ng recombinant human IFN-γ (carr... -
Ultra-LEAF™ Purified anti-mouse IFN-γ
Recombinant Mouse IFN-γ was resolved by electrophoresis, tra...