Ultra-LEAF™ Purified anti-mouse IL-10 Antibody

Pricing & Availability
Clone
JES5-2A5 (See other available formats)
Regulatory Status
RUO
Other Names
Interleukin-10, Cytokine synthesis inhibitory factor (CSIF), B cell derived T cell growth factor (B-TCGF), T cell growth inhibitory factor (TGIF)
Isotype
Rat IgG1, κ
jes5-16e3.jpg
  • jes5-16e3.jpg
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Cat # Size Price Quantity Check Availability
504907 100 µg $110.00
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504908 1 mg $149.00
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504909 5 mg $539.00
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504910 25 mg $1408.00
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504911 50 mg $2354.00
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504912 100 mg $3383.00
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Select size of product is eligible for a 40% discount! Promotion valid until December 31, 2024. Exclusions apply. To view full promotion terms and conditions or to contact your local BioLegend representative to receive a quote, visit our webpage.

Description

IL-10 was originally described as Cytokine Synthesis Inhibitory Factor (CSIF) by virtue of its ability to inhibit cytokine production by Th1 clones. IL-10 shares over 80% sequence homology with the Epstein-Barr virus protein BCRFI. The biological activities of IL-10 include inhibition of macrophage-mediated cytokine synthesis, suppression of the delayed type hypersensitivity response, and stimulation of the Th2 cell response, which results in elevated antibody production. The JES5-2A5 antibody reacts with mouse interleukin-10 (IL-10). The JES5-2A5 antibody can neutralize the bioactivity of natural or recombinant IL-10.

Technical data sheet

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
E. coli-expressed, recombinant mouse IL-10
Formulation
0.2 µm filtered in phosphate-buffered solution, pH 7.2, containing no preservative.
Preparation
The Ultra-LEAF™ (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.
Concentration
The antibody is bottled at the concentration indicated on the vial, typically between 2 mg/mL and 3 mg/mL. Older lots may have also been bottled at 1 mg/mL. To obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C. This Ultra-LEAF™ solution contains no preservative; handle under aseptic conditions.
Application

ELISA Capture - Quality tested
ELISPOT Capture, Neut, WB - Reported in the literature, not verified in house
Recommended Usage

Each lot of this antibody is quality control tested by ELISA assay. For ELISA and ELISPOT capture applications, a concentration range of 4-8 µg/ml is recommended. To obtain a linear standard curve, serial dilutions of IL-10 recombinant protein ranging from 2000 to 15 pg/ml are recommended for each ELISA plate. It is recommended that the reagent be titrated for optimal performance for each application.

* For ELISA/ELISPOT capture, it is very critical to use 0.2 M Sodium Phosphate Buffer, pH 6.5 (11.8g Na2HPO4, 16.1g NaH2PO4; q.s. to 1.0 L) as coating buffer.

Note: Carbonate buffer, pH 9.5 should not be used as coating buffer for JES5-2A5. It may cause high background and lower sensitivity.

Application Notes

ELISA or ELISPOT Capture1-5,7,10: The purified JES5-2A5 antibody is useful as the capture antibody in a sandwich ELISA or ELISPOT assay, when used in conjunction with the biotinylated JES5-16E3 antibody (Cat. No. 505004) as the detecting antibody. The LEAF™ purified antibody is suggested for ELISPOT capture.

ELISA or ELISPOT Detection: The biotinylated JES5-2A5 antibody is useful as the detecting antibody in a sandwich ELISA or ELISPOT assay, when used in conjunction with the Purified JES5-16E3 antibody (Cat. No. 505002/505012) as the capture antibody.

Neutralization2,8,9: The LEAF™ purified antibody (Endotoxin <0.1 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for neutralization of mouse IL-10 bioactivity in vivo and in vitro (Cat. No. 504904).

Additional reported applications (for the relevant formats) include: Western blotting12 and in vivo capture6.

Note: For testing mouse IL-10 in serum, plasma or supernatant, BioLegend's ELISA Max™ Sets (Cat. No. 431401 to 431406) are specially developed and recommended.

Application References

(PubMed link indicates BioLegend citation)
  1. Sander B, et al. 1993. J. Immunol. Meth. 166:201.
  2. Abrams J, et al. 1992. Immunol. Rev. 127:5.
  3. Abrams J, 1995. Curr. Prot. Immunol. John Wiley and Sons, New York. Unit 6.20.
  4. Mo X, et al. 1995. J. Virol. 69:1288.
  5. Sarawar S, et al. 1994. J. Immunol. 153:1246.
  6. Finkelman F, et al. 2003. Curr. Prot. Immunol. John Wiley & Sons, New York. Unit 6.28.
  7. Brummel R and Lenert P, 2005. J. Immunol. 174:2429.
  8. Hara M, et al. 2001. J. Immunol. 166:3789.
  9. Riemann M, et al. 2005. J. Immunol. 175:3560.
  10. Xu G, et al. 2007. J. Immunol. 179:5358.
  11. Guo Z, et al. 2013. J. Immunol. 190:4337. PubMed
  12. van der Poll T, et al. 1995. J. Immunol. 155:5397. (WB)
Product Citations
  1. Song J, et al. 2022. Clin Transl Med. 12:e992. PubMed
  2. Shute A, et al. 2021. Microbiome. 9:186. PubMed
RRID
AB_2810629 (BioLegend Cat. No. 504907)
AB_2810629 (BioLegend Cat. No. 504908)
AB_2810629 (BioLegend Cat. No. 504909)
AB_2810629 (BioLegend Cat. No. 504910)
AB_2810629 (BioLegend Cat. No. 504911)
AB_2810629 (BioLegend Cat. No. 504912)

Antigen Details

Structure
Acid-labile cytokine; dimer; 17-21 kD (Mammalian)
Bioactivity
Inhibits IFN-γ, TNF-β, IL-2 production by TH1 clones; inhibits macrophage-mediated IL-1, IL-6, TNF-α synthesis; suppresses delayed type hypersensitivity response; stimulates TH2 cell response; mast cell proliferation
Cell Sources
Activated CD8+ T cells, TH0, TH2 subset of CD4+ T cells, Ly-1+ B cells, monocytes, macrophages, keratinocytes
Cell Targets
T cells, B cells, mast cells, macrophages
Receptors
IL-10R (CDw210)
Cell Type
Tregs
Biology Area
Immunology
Molecular Family
Cytokines/Chemokines
Antigen References

1. Fitzgerald, K., et al. Eds. 2001. The Cytokine FactsBook. Academic Press, San Diego.
2. de Waal-Malefy, T, et al. 1992. Curr. Opin. Immunol. 4:314.
3. Howard, M., et al. 1992. Immunol. Today. 13:198.
4. Quesniaux, V. 1992. Research Immunol. 143:385.

Regulation
Downregulated by IL-4, IL-10
Gene ID
16153 View all products for this Gene ID
UniProt
View information about IL-10 on UniProt.org

Other Formats

View All IL-10 Reagents Request Custom Conjugation
Description Clone Applications
Purified anti-mouse IL-10 JES5-2A5 ELISA Capture,WB
Biotin anti-mouse IL-10 JES5-2A5 ELISA Detection,ELISPOT Detection,WB
Ultra-LEAF™ Purified anti-mouse IL-10 JES5-2A5 ELISA Capture,ELISPOT Capture,Neut,WB
Go To Top Version: 1    Revision Date: 05/30/2019

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

  • Purified anti-mouse IL-10

    JES5-16E3

  • Biotin anti-mouse IL-10

    JES5-16E3

  • Ultra-LEAF™ Purified anti-mouse IL-10

    jes5-16e3.jpg
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