- Clone
- QA19A66 (See other available formats)
- Regulatory Status
- RUO
- Other Names
- Interleukin-4, B-cell stimulating factor-1 (BSF-1), Ia inducing factor (IaIF), Hodgkin's cell growth factor (HCGF), Mast cell growth factor-2 (MCGF-2), Macrophage fusion factor (MFF), T cell growth factor-2 (TCGF-2)
- Isotype
- Mouse IgG1, κ
-
Recombinant mouse IL-4 (Cat. No. 574306) (black circles) induces the proliferation of HT-2 mouse cells. Ultra-LEAF™ purified anti-mouse IL-4 recombinant antibody (clone QA19A66) (purple squares) inhibits the effect of recombinant mouse IL-4 in mouse HT-2 cells in a dose-dependent manner whereas the GoInVivo™ purified mouse IgG1, κ isotype ctrl antibody (Cat. No. 400192) (gray triangles) does not have the effect. ND50 range: 0.1 - 0.5 µg/mL
| Cat # | Size | Price | Quantity Check Availability | ||
|---|---|---|---|---|---|
| 942803 | 100 µg | $325.00 | |||
| 942804 | 1 mg | $1045.00 | |||
Select size of product is eligible for a 40% discount! Promotion valid until December 31, 2024. Exclusions apply. To view full promotion terms and conditions or to contact your local BioLegend representative to receive a quote, visit our webpage.
IL-4 is a member of the short four-helix bundle family of cytokines. IL-4 is produced by Th2 cells in response to antigen receptor engagement. Dendritic cells can prime naïve CD4+ T cell; nevertheless, it has been described that dendritic cell mediated priming is insufficient for the development of Th2 cell-dependent immunity. Basophils have been identified as a source of IL-4 in the initial naïve CD4+ T cell priming. These cells coexpress MHC class II and IL-4 following helminth infection. Therefore, murine basophils could present antigens to naïve T cells, thus promoting Th2 cell differentiation during allergic and anti-parasitic immune responses. In addition, it was previously known that upon cross-linkage of the high affinity receptor for immunoglobulin E, IL-4 is produced by NK cells, mast cells, and basophils. Cells of hematopoietic stem cell origin express the type I receptor for IL-4 (IL-4Ra, γc), and cells of non-hematopoietic stem cell origin express type II receptor (IL-4Ra, IL-13Ra1). Type I receptor complexes are active in regulating Th2 development. In contrast, the type II receptor complex is active in regulating cells that mediate airway hypersensitivity and mucus secretion.
Product Details
- Verified Reactivity
- Mouse
- Antibody Type
- Recombinant
- Host Species
- Mouse
- Immunogen
- Recombinant mouse IL-4
- Formulation
- 0.2 µm filtered in phosphate-buffered solution, pH 7.2, containing no preservative.
- Preparation
- The Ultra-LEAF™ (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.
- Concentration
- The antibody is bottled at the concentration indicated on the vial, typically between 2 mg/mL and 3 mg/mL. Older lots may have also been bottled at 1 mg/mL. To obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C. This Ultra-LEAF™ solution contains no preservative; handle under aseptic conditions.
- Application
-
Neut - Quality tested
- Recommended Usage
-
Each lot of this antibody is quality control tested by the inhibition of proliferation induced by recombinant mouse IL-4 (Cat. No. 574306) on mouse HT-2 cells. ND50 range: 0.1 - 0.5 µg/mL. It is recommended that the reagent be titrated for optimal performance for each application.
- RRID
-
AB_2910503 (BioLegend Cat. No. 942803)
AB_2910503 (BioLegend Cat. No. 942804)
Antigen Details
- Structure
- Heterodimer, interleukin
- Distribution
-
Th2 cells, NK T cells, mast cells, basophils, eosinophils, macrophages
- Function
- B-cell stimulatory cytokine, Th2 promoting, immunoglobulin isotype switching to IgG1 and IgE, upregulates MHC class II production, T-cell suppressor, regulator in humoral and adaptive immunity, decreases the production of Th1 cells, macrophages, IFN-gamma, and dendritic cell IL-12.
- Interaction
- T cells, B cells, mast cells, monocytes, macrophages, fibroblast, and endothelial cells
- Ligand/Receptor
- Type I receptor complex (IL-4Rα, γc) and type II receptor complex (IL-4Rα, IL-13Rα1)
- Cell Type
- Basophils, Embryonic Stem Cells, Eosinophils, Hematopoietic stem and progenitors, Macrophages, Mast cells, NKT cells
- Biology Area
- Cell Biology, Immunology, Signal Transduction, Stem Cells
- Molecular Family
- Cytokines/Chemokines
- Antigen References
-
- Chen. L, et al. 2004. J. Immunol. 172:2059.
- Maroof A, et al. 2006. Immunology 117:271.
- Sharma P, et al. 2008. Immunity 29:551.
- Heller NM, et al. 2008. Sci. Signal 1:ra17.
- LaPorte SL, et al. 2008. Cell 132:259.
- Perrigoue JG, et al. 2009. Nat. Immunol. 10:697.
- Schneider E, et al. 2010. Eur. Cytokine Netw. 21:142.
- Leavy O. 2011. Nat. Rev. Immunol. doi:10.1038/nri2908.
- Gene ID
- 16189 View all products for this Gene ID
- UniProt
- View information about IL-4 on UniProt.org
Other Formats
View All IL-4 Reagents Request Custom Conjugation| Description | Clone | Applications |
|---|---|---|
| Ultra-LEAF™ Purified anti-mouse IL-4 Recombinant Antibody | QA19A66 | Neut |
Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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Ultra-LEAF™ Purified anti-mouse IL-4 Recombinant Antibody
Recombinant mouse IL-4 (Cat. No. 574306) (black circles) ind...