- Clone
- MM9C1 (See other available formats)
- Regulatory Status
- RUO
- Other Names
- P40, MEA (mast cell growth enhancing activity), MCGF (Mast cell growth factor), TCGF-3 (T cell growth factor-3)
- Isotype
- Mouse IgG2a
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MM9C1 monoclonal anti-mouse IL-9 antibody is able to inhibit mouse IL-9 (1 ng/mL) induced M0-7e cell proliferation with an IC50 < 50 ng/mL.
| Cat # | Size | Price | Quantity Check Availability | ||
|---|---|---|---|---|---|
| 659505 | 100 µg | $237.00 | |||
| 659506 | 1 mg | $688.00 | |||
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IL-9 was initially identified in mice as a T cell growth factor. Human and murine IL-9 show a 56% amino acid sequence homology. IL-9 is a cytokine that acts as a regulator of a variety of hematopoietic cells and stimulates cell proliferation and prevents apoptosis. The IL-9 receptor consists of the cytokine-specific IL-9 receptor α-chain (IL-9Rα) and the common γ-chain that is shared by IL-2, IL-4, IL-7, IL-15, and IL-21. IL-9 is expressed by activated Th9, Th17, Treg, and mast cells and appears to play an important role in the development and function of subsets within the CD4+ T cell lineage. IL-9 also inhibits immune responses by enhancing the suppressive properties of Tregs and by recruiting immune suppressive mast cells to sites of inflammation. Genetic studies on a mouse model of asthma demonstrate that this cytokine is a key factor in the pathogenesis of bronchial hyperresponsiveness. In this sense, it has been described that IL-9 and IL-9R expression are increased in lungs of asthmatic patients. In addition, IL-9 promotes mast cell mediated intestinal permeability and plays a role in the development of food allergies. Also, IL-9 has been associated with atopic dermatitis.
Product Details
- Verified Reactivity
- Mouse
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- Baculovirus-expressed, recombinant mouse IL-9
- Formulation
- 0.2 µm filtered in phosphate-buffered solution, pH 7.2, containing no preservative.
- Preparation
- The Ultra-LEAF™ (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.
- Concentration
- The antibody is bottled at the concentration indicated on the vial, typically between 2 mg/mL and 3 mg/mL. Older lots may have also been bottled at 1 mg/mL. To obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C. This Ultra-LEAF™ solution contains no preservative; handle under aseptic conditions.
- Application
-
FA - Quality tested
Neut - Reported in the literature, not verified in house - Recommended Usage
-
Each lot of this antibody is quality control tested by functional assay. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
-
Additional reported applications (for the relevant formats) include: neutralization4. The Ultra-LEAF™ purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for neutralization of mouse IL-9 bioactivity. MM9C1 is able to neutralize mouse IL-9 induced proliferation of M-07e cells with an IC50 50 ng/mL.
-
Application References
(PubMed link indicates BioLegend citation) -
- Abrams J. 1995. Curr. Prot. Immunol. John Wiley and Sons, New York. Unit 6.20.
- Renauld J, et al. 1995. J. Leuk. Biol. 57:353.
- Sitkauskiene B, et al. 2005. Respir. Res. 6:33.
- Gounni AS, et. al. 2004. J. Immunol. 173:2771. (Neut)
- RRID
-
AB_2876741 (BioLegend Cat. No. 659505)
AB_2876741 (BioLegend Cat. No. 659506)
Antigen Details
- Structure
- Cytokine
- Distribution
-
Th2 cells and mast cells, activated Th9, Th17, and Tregs.
- Function
- Upregulated by IL-1 after TCR activation, also upregulated by TNF-α; downregulated by antibodies to IL-2.
- Interaction
- T helper cells, Th9, Th17, Treg, human airway smooth muscle cells.
- Ligand/Receptor
- Mouse IL-9R
- Cell Type
- Th17, Th2, Tregs
- Biology Area
- Cell Biology, Immunology
- Molecular Family
- Cytokines/Chemokines
- Antigen References
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1. Goswami R. and Kaplan MH. 2011. J. Immunol. 186:3283.
2. Kaiko GE, and Foster PS. 2011. Curr. Opin. Allergy Clin. Immunol. 11:39.
3. Townsend JM, et al. 2000. Immunity. 13:573.
4. Mock BA, et al. 1990. Immunogenetics. 31:265.
5. Tan C, et al. 2010. J. Immunol. 185:6795.
6. Jager A, et al. 2009. J. Immunol. 183:7169. - Gene ID
- 16198 View all products for this Gene ID
- UniProt
- View information about IL-9 on UniProt.org
Other Formats
View All IL-9 Reagents Request Custom Conjugation| Description | Clone | Applications |
|---|---|---|
| Ultra-LEAF™ Purified anti-mouse IL-9 | MM9C1 | FA,Neut |
Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
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Ultra-LEAF™ Purified anti-mouse IL-9
MM9C1 monoclonal anti-mouse IL-9 antibody is able to inhibit...