- Clone
- QA19A26 (See other available formats)
- Regulatory Status
- RUO
- Other Names
- Tumor necrosis factor-α, Cachectin, Necrosin, Macrophage cytotoxic factor (MCF), Differentiation inducing factor (DIF), TNFSF2
- Isotype
- Mouse IgG1, κ
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Recombinant mouse TNF-α (Cat. No. 575206) (black circles) cytotoxicity of 929L mouse musculus cells. Ultra-LEAF™ purified anti-mouse TNF-α antibody (clone QA19A26) (purple squares) neutralizes the cytotoxicity function of recombinant mouse TNF-α at 0.03 ng/mL in 929L cells in a dose-dependent manner. ND50 range: 0.5-2.5 μg/mL.
| Cat # | Size | Price | Quantity Check Availability | ||
|---|---|---|---|---|---|
| 935903 | 100 µg | $270.00 | |||
| 935904 | 1 mg | $715.00 | |||
Select size of product is eligible for a 40% discount! Promotion valid until December 31, 2024. Exclusions apply. To view full promotion terms and conditions or to contact your local BioLegend representative to receive a quote, visit our webpage.
TNF-α is secreted by macrophages, monocytes, neutrophils, T-cells, and NK-cells. Many transformed cell lines also secrete TNF-α. TNF-α forms multimeric complexes; stable trimers are most common in solution. A 26 kD membrane form of TNF-α has also been described. TNF-α binding to surface receptors elicits a wide array of biologic activities including: cytolysis and cytostasis of many tumor cell lines in vitro, hemorraghic necrosis of tumors in vivo, increased fibroblast proliferation, and enhanced chemotaxis and phagocytosis in neutrophils.
Product Details
- Verified Reactivity
- Mouse
- Antibody Type
- Recombinant
- Host Species
- Mouse
- Immunogen
- Recombinant Mouse TNF-α
- Formulation
- 0.2 µm filtered in phosphate-buffered solution, pH 7.2, containing no preservative.
- Concentration
- The antibody is bottled at the concentration indicated on the vial, typically between 2 mg/mL and 3 mg/mL. Older lots may have also been bottled at 1 mg/mL. To obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C. This Ultra-LEAF™ solution contains no preservative; handle under aseptic conditions.
- Application
-
Neut - Quality tested
- Recommended Usage
-
Each lot of this antibody is quality control tested by neutralization of the cytotoxicity by recombinant mouse TNF- α (0.03 ng/mL) on 929L mouse musculus cells. ND50 range: 0.5 - 2.5 µg/mL. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
-
This recombinant antibody clone is derived from clone MP6-XT22.
- RRID
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AB_2890855 (BioLegend Cat. No. 935903)
AB_2890855 (BioLegend Cat. No. 935904)
Antigen Details
- Structure
- TNF superfamily; dimer/trimer
- Distribution
-
Activated monocytes, neutrophils, macrophages, T cells, B cells, NK cells, LAK cells
- Function
- Type II integral membrane protein processed by TACE for secretion; upregulated by interferons, IL-2, GM-CSF, substance P, bradykinin, PAF, immune complexes, cyclooxygenase; downregulated by IL-6, TGF-β, vitamin D3, prostaglandin E2, PAF antagonists
- Interaction
- Monocytes, neutrophils, macrophages, T cells, fibroblasts, endothelial cells, osteoclasts, adipocytes, astroglia, microglia
- Ligand/Receptor
- TNFRSF1A (TNF-R1, CD120a, TNFR-p60 Type β, p55); TNFRSF1B (TNF-R2, CD120b, TNFR-p80 Type A, p75)
- Cell Type
- B cells, Endothelial cells, Fibroblasts, Macrophages, Microglia, Monocytes, Neutrophils, NK cells, Osteoclasts, T cells
- Biology Area
- Cell Biology, Immunology, Neuroinflammation, Neuroscience
- Molecular Family
- Cytokines/Chemokines
- Antigen References
-
1. Fitzgerald K, et al. Eds. 2001. The Cytokine FactsBook. Academic Press San Diego.
2. Beutler B, et al. 1988. Annu. Rev. Biochem. 57:505.
3. Beutler B, et al. 1989. Annu. Rev. Immunol. 7:625.
4. Tracey K, et al. 1993. Crit. Care Med. 21:S415. - Gene ID
- 21926 View all products for this Gene ID
- UniProt
- View information about TNF-alpha on UniProt.org
Other Formats
View All TNF-α Reagents Request Custom Conjugation| Description | Clone | Applications |
|---|---|---|
| Ultra-LEAF™ Purified anti-mouse TNF-α Recombinant Antibody | QA19A26 | Neut |
Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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Ultra-LEAF™ Purified anti-mouse TNF-α Recombinant Antibody
Recombinant mouse TNF-α (Cat. No. 575206) (black circles) cy...