- Clone
- 2G11-2A05 (See other available formats)
- Regulatory Status
- RUO
- Other Names
- Vascular endothelial growth factor (VEGF)
- Isotype
- Rat IgG2a, κ
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Western blot analysis of 50 ng of recombinant mouse VEGF-164 (lane 1), recombinant mouse VEGF-120 (lane 2), recombinant human VEGF-165 (lane 3), recombinant human VEGF-121 (lane 4), and recombinant human VEGF-C (lane 5) using LEAF™ purified anti-mouse VEGF-A (clone 2G11-2A05) antibody.
| Cat # | Size | Price | Quantity Check Availability | ||
|---|---|---|---|---|---|
| 512809 | 100 µg | $270.00 | |||
| 512810 | 1 mg | $715.00 | |||
Select size of product is eligible for a 40% discount! Promotion valid until December 31, 2024. Exclusions apply. To view full promotion terms and conditions or to contact your local BioLegend representative to receive a quote, visit our webpage.
VEGF-A, also known as VEGF (vascular endothelial growth factor), is the founding member of the family including VEGF (VEGF-A), VEGF-B, VEGF-C, VEGF-D, VEGF-E, and PIGF. Vascular endothelial growth factors (VEGFs) are a family of secreted polypeptides with a highly conserved receptor-binding cystine-knot structure similar to that of the platelet-derived growth factors.VEGF-A has important roles in mammalian vascular development and in diseases involving abnormal growth of blood vessels such as tumor-related angiogenesis.VEGF-A is produced by diverse cell types, including aortic vascular smooth muscle cells, keratinocytes, macrophages and many tumor cells. Oxygen tension is a key physiological regulator of VEGF-A gene expression.
Product Details
- Verified Reactivity
- Mouse, Human
- Antibody Type
- Monoclonal
- Host Species
- Rat
- Immunogen
- N-terminal 24 aa sequence of murine VEGF
- Formulation
- 0.2 µm filtered in phosphate-buffered solution, pH 7.2, containing no preservative.
- Preparation
- The Ultra-LEAF™ (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.
- Concentration
- The antibody is bottled at the concentration indicated on the vial, typically between 2 mg/mL and 3 mg/mL. Older lots may have also been bottled at 1 mg/mL. To obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C. This Ultra-LEAF™ solution contains no preservative; handle under aseptic conditions.
- Application
-
WB - Quality tested
Neut, ELISA, IHC-F, FA - Reported in the literature, not verified in house - Recommended Usage
-
Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.5 - 2.0 µg/ml. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
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Neutralization: The Ultra-LEAF™ Purified antibody is recommended for neutralization of mouse VEGF-A bioactivity in vivo2,4 and in vitro2.
Western Blotting: It is suggested to use 0.5 - 2 µg/ml for WB2.
Immunohistochemistry: Clone 2G11-2A05 is recommended for IHC on saponin-treated frozen tissue sections at a concentration range of 5-10 µg/ml.
ELISA or ELISPOT: The Ultra-LEAF™ Purified 2G11-2A05 antibody is useful in a mouse VEGF-A ELISA or ELISPOT assay.
Functional Assay: The Ultra-LEAF™ Purified 2G11-2A05 antibody is useful in a mouse VEGF-A functional assay2. -
Application References
(PubMed link indicates BioLegend citation) -
- Wuest TR, et al. 2009. J. Exp Med. 207;101. (Neut) PubMed
- Basu A, et al. 2008. Cancer Res. 68:5689. (Neut, WB, FA)
- Kumar V, et al. 2010. Blood 115:4725. PubMed
- Lu R, et al. 2012. Cancer Res. 72:2239. (Neut) PubMed
- Jayarman P, et al. 2012. J. Immunol. 188:5365. PubMed
- Kather JN, et al. 2014. Invest Ophthalmol Vis Sci. 55:4295. PubMed
- Product Citations
-
- RRID
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AB_2814440 (BioLegend Cat. No. 512809)
AB_2814440 (BioLegend Cat. No. 512810)
Antigen Details
- Structure
- Cystine-knot growth factors
- Distribution
-
Aortic vascular smooth muscle cells, keratinocytes, macrophages and many tumor cells
- Function
- Angiogenesis
- Cell Targets
- Endothelial cells
- Receptors
- VEGFR-1 (Flt-1) and VEGFR-2 (KDR/Flk-1)
- Cell Type
- Macrophages
- Biology Area
- Angiogenesis, Cell Biology, Immunology
- Molecular Family
- Cytokines/Chemokines, Growth Factors
- Antigen References
-
1. Contreras A, et al. 2007. Am. J. Transpl. 7:155.
2. Lambrechts D and Carmeliet P. 2007. Biochim. Biophys. Acta. 1762:1109.
3. Tong JP and Yao YF. 2006. Clin. Biochem. 39:267.
4. Ferrara N, et al. 2003. Nat. Med. 9:669.
5. Shibuya M. 2001. Cell Struct. Funct. 26:25.
6. Senger DR, et al. 1983. Science. 219:983. - Regulation
- HIF-1, hypoxiaresponsiveness
- Gene ID
- 22339 View all products for this Gene ID
- UniProt
- View information about VEGF-A on UniProt.org
Other Formats
View All VEGF-A Reagents Request Custom Conjugation| Description | Clone | Applications |
|---|---|---|
| Ultra-LEAF™ Purified anti-mouse VEGF-A | 2G11-2A05 | WB,Neut,ELISA,IHC-F,FA |
Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
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Ultra-LEAF™ Purified anti-mouse VEGF-A
Western blot analysis of 50 ng of recombinant mouse VEGF-164...