- Clone
- 2E11 (See other available formats)
- Regulatory Status
- RUO
- Other Names
- Thy-1, Thy1
- Isotype
- Mouse IgG1, κ
-
LOU rat thymocytes were stained with purified CD90 (clone 2E11, filled histogram) or purified mouse IgG1, κ isotype control (open histogram) followed by anti-mouse IgG PE.
| Cat # | Size | Price | Quantity Check Availability | ||
|---|---|---|---|---|---|
| 206104 | 500 µg | $270.00 | |||
Select size of product is eligible for a 40% discount! Promotion valid until December 31, 2024. Exclusions apply. To view full promotion terms and conditions or to contact your local BioLegend representative to receive a quote, visit our webpage.
CD90, also known as Thy-1, is a 28-30 kD GPI-linked membrane glycoprotein. It is a member of the immunoglobulin superfamily and has been shown to interact with CD45 in signal transduction during lymphocyte proliferation and differentiation. CD90 is expressed on hematopoietic stem cells, neurons, thymocytes, peripheral T cells, fibroblasts, and stromal cells.
Product Details
- Verified Reactivity
- Rat
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- PC12 cells.
- Formulation
- 0.2 µm filtered in phosphate-buffered solution, pH 7.2, containing no preservative.
- Preparation
- The Ultra-LEAF™ (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.
- Concentration
- The antibody is bottled at the concentration indicated on the vial, typically between 2 mg/mL and 3 mg/mL. Older lots may have also been bottled at 1 mg/mL. To obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C. This Ultra-LEAF™ solution contains no preservative; handle under aseptic conditions.
- Application
-
FC - Quality tested
IP, WB - Reported in the literature, not verified in house - Recommended Usage
-
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.03 µg per million cells in 100 µl volume or 100 µl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
-
Additional reported applications (for relevant formats of this clone) include: immunoprecipitation and Western blotting.
For in vivo studies or highly sensitive assays, we recommend Ultra-LEAF™ purified antibody (Cat. No. 206104) with a lower endotoxin limit than standard LEAF™ purified antibodies (Endotoxin <0.01 EU/µg) -
Application References
(PubMed link indicates BioLegend citation) -
- Kuroiwa K, et al. 2012. Hybridoma 31:225.
- RRID
-
AB_2565936 (BioLegend Cat. No. 206104)
Antigen Details
- Structure
- Ig superfamily, GPI-anchored membrane glycoprotein.
- Distribution
-
Hematopoietic stem cells, thymocytes, a subset of peripheral blood T cells, early myeloid and erythroid progenitors, immature B cells, neurons, activated endothelium, mast cells, dendritic cells, and glomeruli.
- Function
- Adhesion, signal transduction, lymphocyte co-stimulation, and proliferation and differentiation of hematopoietic stem cells.
- Ligand/Receptor
- Interacts with CD45.
- Cell Type
- B cells, Dendritic cells, Hematopoietic stem and progenitors, Mast cells, Neurons, T cells, Thymocytes
- Biology Area
- Immunology
- Molecular Family
- Adhesion Molecules, CD Molecules
- Antigen References
-
1. Campbell DG, et al. 1981. Biochem. J. 195:15.
2. Hosseinzadeh H, et al. 1993. J. Immunol. 150:1670. - Gene ID
- 21838 View all products for this Gene ID
- UniProt
- View information about CD90 on UniProt.org
Other Formats
View All CD90 Reagents Request Custom Conjugation| Description | Clone | Applications |
|---|---|---|
| Purified anti-rat CD90 | 2E11 | FC,IP,WB |
| Ultra-LEAF™ Purified anti-rat CD90 | 2E11 | FC,IP,WB |
| FITC anti-rat CD90 | 2E11 | FC |
| Alexa Fluor® 647 anti-rat CD90 | 2E11 | FC,IHC-F |
Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
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Purified anti-rat CD90
LOU rat thymocytes were stained with purified CD90 (clone 2E... -
Ultra-LEAF™ Purified anti-rat CD90
LOU rat thymocytes were stained with purified CD90 (clone 2E... -
FITC anti-rat CD90
LOU rat thymocytes were stained with rat CD90 (clone 2E11) F... -
Alexa Fluor® 647 anti-rat CD90
LOU rat thymocytes were stained with anti-rat CD90 (clone 2E... 
LOU rat frozen thymus section was fixed with 4% paraformalde...