- Clone
- A20103J (See other available formats)
- Regulatory Status
- RUO
- Other Names
- Spike glycoprotein, S glycoprotein (E2)
- Isotype
- Rat IgG2a, κ
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Recombinant human ACE2 (Cat. No. 792002) (black circles) binds to immobilized recombinant SARS-CoV-2 S protein S1-Fc chimera (Cat. No. 793004) in a dose-dependent manner. Purified anti-SARS-CoV-2 S protein S1 antibody (clone A20103J) (purple squares) inhibits the binding in a dose-dependent manner. This antibody blocks the binding of 0.5 µg/mL recombinant human ACE2 to 1.0 µg/mL immobilized recombinant SARS-CoV-2 S protein S1-Fc chimera. ND50 range: 0.04 - 0.2 µg/mL.
| Cat # | Size | Price | Quantity Check Availability | ||
|---|---|---|---|---|---|
| 944703 | 100 µg | $358.00 | |||
| 944704 | 1 mg | $1205.00 | |||
Select size of product is eligible for a 40% discount! Promotion valid until December 31, 2024. Exclusions apply. To view full promotion terms and conditions or to contact your local BioLegend representative to receive a quote, visit our webpage.
Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is a single-stranded RNA virus that belongs in a family of viruses known as coronaviruses. SARS-CoV-2 infection, known as COVID-19, was declared a pandemic by WHO on March 11, 2020 and causes symptoms leads to respiratory infection, pulmonary failure which can be fatal. SARS-CoV-2 is structurally composed of 4 main proteins (e.g. spike glycoprotein, envelope glycoprotein, membrane glycoprotein and nucleocapsid protein) and several accessory proteins. The spike glycoprotein (S) is a transmembrane molecule that forms homotrimers on the surface of the virus and facilitates virulence through binding to angiotensin-converting enzyme 2 (ACE2) expressed on host cells. This protein is composed of two subunits S1 and S2. S1 is responsible for binding to the host cell via ACE2 receptor and the S2 subunit is responsible for fusion with the host cell.
Product Details
- Verified Reactivity
- SARS-CoV-2
- Antibody Type
- Monoclonal
- Host Species
- Rat
- Immunogen
- SARS-CoV-2
- Formulation
- 0.2 µm filtered in phosphate-buffered solution, pH 7.2, containing no preservative.
- Concentration
- The antibody is bottled at the concentration indicated on the vial, typically between 2 mg/mL and 3 mg/mL. Older lots may have also been bottled at 1 mg/mL. To obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C. This Ultra-LEAF™ solution contains no preservative; handle under aseptic conditions.
- Application
-
Blocking - Quality tested
- Recommended Usage
-
Each lot of this antibody is quality control tested by blocking the binding of 0.5 µg/mL recombinant human ACE2 (Cat. No. 792002) to 1 µg/mL immobilized recombinant SARS-CoV-2 S protein S1-Fc chimera (Cat. No. 793004). ND50 range: 0.04 - 0.2 µg/mL. It is recommended that the reagent be titrated for optimal performance for each application.
- RRID
-
AB_2890874 (BioLegend Cat. No. 944703)
AB_2890874 (BioLegend Cat. No. 944704)
Antigen Details
- Structure
- Spike glycoprotein is a homotrimer. Each monomer consists of 1,273 a.a with a molecular weight of approximately 141 kD and consists of S1 and S2 subunits. S1 subunit is 673 a.a. long.
- Distribution
-
Viral envelope protein, host cell membrane, host cell endoplasmic reticulum-Golgi intermediate compartment membrane
- Function
- Host cell surface receptor binding
- Interaction
- ACE2
- Ligand/Receptor
- ACE2
- Biology Area
- COVID-19
- Antigen References
-
1. Walls AC, et al. 2020. Cell. 181(2):281-292.
2. Yan R, et al. 2020. Science. 367 (6485):1444-1448.
3. Wrapp D, et al. 2020. Science. 367 (6483):1260-1263.
4. Shang J, et al. 2020. PNAS. 117(21):11727-11734 - Gene ID
- 43740568 View all products for this Gene ID
- UniProt
- View information about Viral Protein on UniProt.org
Other Formats
View All Viral Protein Reagents Request Custom Conjugation| Description | Clone | Applications |
|---|---|---|
| Ultra-LEAF™ Purified anti-SARS-CoV-2 S Protein S1 Antibody | A20103J | Block |
Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
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Ultra-LEAF™ Purified anti-SARS-CoV-2 S Protein S1 Antibody
Recombinant human ACE2 (Cat. No. 792002) (black circles) bin...