Antibody-DNA Oligo conjugates are enabling a new era of single cell analysis. Single cell multiomics now allows for simultaneous profiling of RNA and protein from single cells. CITE-Seq (Cellular Indexing of Transcriptomes and Epitopes by Sequencing) and similar methods seamlessly integrate antibody-oligo conjugates with existing single-cell sequencing workflows allowing antibody directed quantification of cellular proteins simultaneously with the RNA transcriptome.

BioLegend’s revolutionary TotalSeq™ oligo conjugated antibodies provide a standardized process for antibody directed protein characterization. This allows for measurement of cell surface proteins without the multiplexing limitation of fluorophores or heavy metals; more than 220 proteins currently. TotalSeq™ Cell Hashing antibodies also allow for sample multiplexing by targeting ubiquitously expressed surface proteins. TotalSeq™ Cell Hashing antibodies combined with antibody directed protein analysis enable extensive sample data cells incorporating targets from both the proteome and transcriptome of single cells.

Join us to review the use of TotalSeq™ antibodies in exciting applications such as CITE-seq, and, how it interfaces with commercial scRNAseq solutions such as the 10X Genomics system with Feature Barcoding Technology™.  We will discuss technical considerations, collaborator data, new products in development, and more.

BioLegend Seminar: TotalSeq™, Revolutionary Highly Multiplexed Protein Analysis in Single-Cell Multi-Omics

Date: April 21, 2020 at 11:00 AM EST

Speaker: Josh Croteau, PhD, Technical Applications Scientist II, BioLegend Inc.