Direct-Blot™

When performing a western blot, secondary antibodies are commonly used to detect your protein of interest. However, unless the signal needs to be amplified, there is no advantage to using a two-step process. Our Direct-Blot™ reagents are horseradish peroxidase (HRP)-conjugated primary antibodies that eliminate the need for a secondary antibody. By removing several steps from the workflow, they shorten the protocol and help you get your results faster. Our scientists have carefully developed and tested these antibodies to ensure they provide high sensitivity and excellent stability.

 

Follow our Direct-Blot™ protocol and learn more about Direct-Blot™ antibodies.

Direct-Blot™ HRP anti-β-Actin Antibody

Whole cell lysates (15 µg protein) from HeLa cultures were resolved by electrophoresis (4-20% Trisglycine gel), transferred to nitrocellulose, and probed with 1:10,000, 1:40,000, and 1:100,000 dilutions of Direct-Blot™ HRP anti-β-actin Antibody, clone W16197A, or a 1:10,000 dilution of purified anti-β-actin Antibody, clone W16197A (upper). Proteins were visualized using a 1:3000 diluted HRP anti rat-IgG secondary antibody for purifi ed anti-β-actin Antibody and chemiluminescence detection. Ponceau S staining was used as loading control (lower). Lane M: MW ladder.

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