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Brilliant Violet 421™ anti-human LOX-1 Antibody

Pricing & Availability
Clone
15C4 (See other available formats)
Regulatory Status
RUO
Other Names
OLR1, LOX1, LOXIN, SLOX1, CLEC8A, SCARE1
Isotype
Mouse IgG2a, κ
Ave. Rating
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Product Citations
publications
15C4_BV421_LOX-1_Antibody_032118
GM-CSF-stimulated (3 days) human peripheral blood monocytes were stained with LOX-1 (clone 15C4) Brilliant Violet 421™ (filled histogram) or mouse IgG2a, κ Brilliant Violet 421™ isotype control (open histogram).
  • 15C4_BV421_LOX-1_Antibody_032118
    GM-CSF-stimulated (3 days) human peripheral blood monocytes were stained with LOX-1 (clone 15C4) Brilliant Violet 421™ (filled histogram) or mouse IgG2a, κ Brilliant Violet 421™ isotype control (open histogram).
Compare all formats See Brilliant Violet 421™ spectral data
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358609 25 tests 164€
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358610 100 tests 348€
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Description

Lectin-like oxidized LDL receptor 1 (LOX-1) also known as CLEC8A is a 31 kD single pass type II transmembrane protein and member of the C-type lectin superfamily. LOX-1 is a scavenger receptor and is involved in endocytosis, phagocytosis and cytokine production. CLEC8A is expressed by endothelial cells, smooth muscle cells, platelets, fibroblasts, macrophages, and is upregulated by inflammatory and oxidative stimuli. LOX-1 recognizes numerous ligands including lipoproteins, apoptotic cells heat shock proteins, C-reactive protein and oxidized LDL. LOX-1 is implicated in atherosclerosis and vascular inflammation.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
LOX-1 ectodomain: Fc fusion protein
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA).
Preparation
The antibody was purified by affinity chromatography and conjugated with Brilliant Violet 421™ under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

Brilliant Violet 421™ excites at 405 nm and emits at 421 nm. The standard bandpass filter 450/50 nm is recommended for detection. Brilliant Violet 421™ is a trademark of Sirigen Group Ltd.


Learn more about Brilliant Violet™.

This product is subject to proprietary rights of Sirigen Inc. and is made and sold under license from Sirigen Inc. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. Patent(s), pending patent applications and foreign equivalents.
Excitation Laser
Violet Laser (405 nm)
Application Notes

Additional reported applications (for the relevant formats) include: immunofluorescent staining of acetone-fixed frozen human skin sections1.

Application References
  1. Li D, et al. 2012. J. Exp. Med. 209:109. (IHC)
Product Citations
  1. Emmons TR, et al. 2021. Cancer Immunol Res. 9:790. PubMed
  2. Koh J, et al. 2020. Eur J Immunol. 50:1810. PubMed
RRID
AB_2728342 (BioLegend Cat. No. 358609)
AB_2728342 (BioLegend Cat. No. 358610)

Antigen Details

Structure
Single pass, type II, transmembrane protein, 31 kD, homodimer, member of the C-type lectin superfamily.
Distribution
Endothelial cells, smooth muscle, platelets, fibroblasts, macrophages; Upregulated by inflammatory and oxidative stimuli
Function
Scavenger receptor, involved in endocytosis, phagocytosis and cytokine production
Ligand/Receptor
Recognizes numerous ligands including lipoproteins, apoptotic cells, heat shock proteins, C-reactive protein, oxidized LDL
Cell Type
Endothelial cells, Platelets, Fibroblasts, Macrophages, Dendritic cells
Biology Area
Cell Adhesion, Cell Biology, Immunology, Innate Immunity
Molecular Family
Adhesion Molecules
Antigen References

1. Yin Y, et al. 2013. Eur. J. Cell Biol. 92:150. 
2. Khaidakov M. and Mehta JL. 2012. PLoS One. 7:e46973. 
3. Sawamura T, et al. 2012. Curr. Opin. Lipidol. 23:439. 
4. Lin FY, et al. 2011. J. Immunol. 186:4405. 
5. Parlato S, et al. 2010. Blood 115:1554.

Gene ID
4973 View all products for this Gene ID
Specificity (DOES NOT SHOW ON TDS):
LOX-1
Specificity Alt (DOES NOT SHOW ON TDS):
LOX-1
App Abbreviation (DOES NOT SHOW ON TDS):
FC
UniProt
View information about LOX-1 on UniProt.org

Related FAQs

What is the F/P ratio range of our BV421™ format antibody reagents?

It is lot-specific. On average it ranges between 2-4.

Go To Top Version: 0    Revision Date: 03.21.2018

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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