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LEAF™ Purified anti-mouse IFN-γ Antibody

Pricing & Availability
Clone
R4-6A2 (See other available formats)
Regulatory Status
RUO
Other Names
Interferon-γ, Immune interferon, Type II interferon, T cell interferon, Macrophage-activating factor (MAF), IFN-g, IFN-gamma
Isotype
Rat IgG1, κ
Ave. Rating
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Product Citations
publications
R4-6A2
  • R4-6A2
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This product has been discontinued as a part of our ongoing LEAF™ to Ultra-LEAF™ antibody transition.
The equivalent Ultra-LEAF™ product may be available at comparable or significantly lower price.

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Description

Interferon-γ is a potent multifunctional cytokine which is secreted primarily by activated NK cells and T cells. Originally characterized based on anti-viral activities, IFN-γ also exerts anti-proliferative, immunoregulatory, and proinflammatory activities. IFN-γ can upregulate MHC class I and II antigen expression by antigen-presenting cells.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Partially-purified, native mouse IFN-γ
Formulation
0.2 µm filtered in phosphate-buffered solution, pH 7.2, containing no preservative. Endotoxin level is <0.1 EU/µg of the protein (<0.01 ng/µg of the protein) as determined by the LAL test.
Preparation
The LEAF™ (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.
Concentration
1.0 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C. This LEAF™ solution contains no preservative; handle under aseptic conditions.
Application

ELISA Capture - Quality tested
ELISPOT Capture, Neut, IHC - Reported in the literature, not verified in house
Recommended Usage

Each lot of this antibody is quality control tested by ELISA assay. For ELISA capture applications, a concentration range of 0.5-2.0 µg/ml is recommended. To obtain a linear standard curve, serial dilutions of IFN-γ recombinant protein ranging from 2000 to 15 pg/ml are recommended for each ELISA plate. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

ELISA Capture1-4,6,10,11 or ELISPOT Capture5: The purified R4-6A2 antibody is useful as the capture antibody in a sandwich ELISA or ELISPOT assay, when used in conjunction with the biotinylated XMG1.2 antibody (Cat. No. 505804) as the detecting antibody and recombinant mouse IFN-? (Cat. No. 575309) as the standard. The Ultra-LEAF™ purified antibody is suggested for ELISPOT capture. For ELISPOT Capture, the suggested use of this antibody is 1.0 - 4.0 µg/mL.
ELISA or ELISPOT Detection: The biotinylated R4-6A2 antibody is useful as the detecting antibody in a sandwich ELISA or ELISPOT assay, when used in conjunction with the purified XMG1.2 antibody (Cat. Nos. 505802 & 505812) as the capture antibody and recombinant mouse IFN-? (Cat. No. 575309) as the standard.
Neutralization1,2,9: The Ultra-LEAF™ purified antibody (Endotoxin <0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for neutralization of mouse IFN-? bioactivity in vivo and in vitro (Cat. Nos. 505708 - 505713).
Additional reported applications (for the relevant formats) include: immunohistochemical staining3,6,8 of acetone-fixed frozen tissue sections.
Note: For testing mouse IFN-? in serum, plasma or supernatant, BioLegend's ELISA Max™ Sets (Cat. No. 430801 and 430804) are specially developed and recommended.

Application References
  1. Abrams J, et al. 1992. Immunol. Rev. 127:5.
  2. Stevenson M, et al. 1990. Infect. Immun. 58:3225.
  3. Sander B, et al. 1993. J. Immunol. Meth. 166:201.
  4. Yang X, et al. 1993. J. Immunoassay 14:129.
  5. Klinman D, et al. 1994. Curr. Prot. Immunol.. John Wiley and Sons, New York. Unit 6.19.
  6. Sander B, et al. 1991. Immunol. Rev. 119:65.
  7. Finkelman F, et al. 2003. Curr. Prot. Immunol. John Wiley & Sons, New York. Unit 6.28.
  8. Khanna A, et al. 2000. J. Immunol. 164:1346.
  9. Terrazas LI, et al. 2005. Intl. J. Parasitology. 35:1349.
  10. Dzhagalov I, et al. 2007. J. Immunol. 178:2113.
  11. Xu G, et al. 2007. J. Immunol. 179:5358.
Product Citations
  1. Lombes A, et al. 2015. J Immunol. 195: 1449-1458. PubMed
  2. Park TY, et al. 2019. Sci Rep. 9:15559. PubMed
  3. Cho S, et al. 2017. Sci Rep. 7:35. PubMed
  4. Chou T, et al. 2016. Nat Commun. 7:11904. PubMed
  5. Mastroianni J, et al. 2019. Cancer Res. 79:183. PubMed
  6. Clua P, et al. 2017. Front Immunol. 10.3389/fimmu.2017.00948. PubMed
  7. Thornton T, et al. 2016. Nat Commun. 7:10553. PubMed
  8. Katsuyama T, et al. 2021. Cell Reports. 36(1):109339. PubMed

Antigen Details

Structure
Cytokine; dimer; 40-80 kD (Mammalian)
Bioactivity
Antiviral/antiparasitic activities; inhibits proliferation; enhances MHC class I and II expression on APC
Cell Sources
CD8+ and CD4+ T cells, NK cells
Cell Targets
T cells, B cells, macrophages, NK cells, endothelial cells, fibroblasts
Receptors
IFN-γRα (CDw119) dimerized with IFN-γRβ (AF-1)
Cell Type
Tregs
Biology Area
Cell Biology, Immunology, Neuroinflammation, Neuroscience
Molecular Family
Cytokines/Chemokines
Antigen References
  1. Fitzgerald K, et al. Eds. 2001. The Cytokine FactsBook. Academic Press, San Diego.
  2. De Maeyer E, et al. 1992. Curr. Opin. Immunol. 4:321.
  3. Farrar M, et al. 1993. Annu. Rev. Immunol. 11:571.
  4. Gray P, et al. 1987. Lymphokines 13:151.
Regulation
Upregulated by IL-2, FGF-basic, EGF; downregulated by 1-α-25-Dihydroxy vitamin D3, dexamethasone
Gene ID
15978 View all products for this Gene ID
Specificity (DOES NOT SHOW ON TDS):
IFN-gamma
Specificity Alt (DOES NOT SHOW ON TDS):
IFN-γ
App Abbreviation (DOES NOT SHOW ON TDS):
ELISA Capture, ELISPOT Capture, IHC, Neut
UniProt
View information about IFN-gamma on UniProt.org

Related FAQs

Do you guarantee that your antibodies are totally pathogen free?

BioLegend does not test for pathogens in-house aside from the GoInVivo™ product line. However, upon request, this can be tested on a custom basis with an outside, independent laboratory.

Does BioLegend test each Ultra-LEAF™ antibody by functional assay?

No, BioLegend does not test Ultra-LEAF™ antibodies by functional assays unless otherwise indicated. Due to the possible complexities and variations of uses of biofunctional antibodies in different assays and because of the large product portfolio, BioLegend does not currently perform functional assays as a routine QC for the antibodies. However, we do provide references in which the antibodies were used for functional assays and we do perform QC to verify the specificity and quality of the antibody based on our strict specification criteria.

Does BioLegend test each Ultra-LEAF™ antibody for potential pathogens?

No, BioLegend does not test for pathogens in-house unless otherwise indicated.  However, we can recommend an outside vendor to perform this testing as needed.

Have you tested this Ultra-LEAF™ antibody for in vivo or in vitro applications?

We don't test our antibodies for in vivo or in vitro applications unless otherwise indicated. Depending on the product, the TDS may describe literature supporting usage of a particular product for bioassay. It may be best to further consult the literature to find clone specific information.

Other Formats

View All IFN-γ Reagents Request Custom Conjugation
Description Clone Applications
Biotin anti-mouse IFN-γ R4-6A2 ELISA Detection,ELISPOT Detection
Purified anti-mouse IFN-γ R4-6A2 ELISA Capture,IHC
Ultra-LEAF™ Purified anti-mouse IFN-γ R4-6A2 ELISA Capture,ELISPOT Capture,IHC-F,Neut
Go To Top Version: 3    Revision Date: 02.25.2014

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

  • Biotin anti-mouse IFN-γ

    R4-6A2

  • Purified anti-mouse IFN-γ

    R4-6A2

  • Ultra-LEAF™ Purified anti-mouse IFN-γ

    R4-6A2
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