PE anti-mouse CD326 (Ep-CAM) Antibody

Product Details

Verified Reactivity

mouse

Antibody Type

Monoclonal

Host Species

Rat

Immunogen

TE-71 thymic epithelial cell line

Formulation

Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.

Preparation

The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions.

Concentration

0.2 mg/ml

Storage & Handling

The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤1.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser

Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)

Application Notes

Additional reported applications for clone G8.8 (for the relevant formats) include: immunohistochemistry of frozen sections: acetone fixed¹, with or without OCT embedding^2,4, and spatial biology (IBEX)^13,14.

Application References

1. Farr A, et al. 1991. J. Histochem. Cytochem. 39:645. (FC, IHC)
2. Dooley J, et al. 2005. J. Immunol. 175:4331. (FC, IHC)
3. Hinterberger M, et. al. 2010. Nat. Immunol. 11:512. (FC) PubMed
4. Gracz AD, et al. 2010. Am J. Physiol Gastrointest Liver Physiol. 298:590. (IHC) PubMed
5. Nudel I, et al. 2011. J. Immunol. 186:891. PubMed
6. Morimoto H, et al. 2012. Biol Reprod. 86:148. PubMed
7. Ishii K, et al. 2012. Development. 139:1734. PubMed
8. Takehashi M, et al. 2012. Biol Reprod. 86:178. PubMed
9. Murakami R, et al. 2013. PLoS One. 8:73270. PubMed
10. Taguchi K, et al. 2014. Mol Cell Biol. 34:900. PubMed
11. Hirokawa Y, et al. 2014. Am J Physiol Gastrointerest Liver Physiol. 306:547. PubMed
12. Ding X, et al. 2015. Cancer Res. 75:330. PubMed
13. Radtke AJ, et al. 2020. Proc Natl Acad Sci U S A. 117:33455-65. (SB) PubMed
14. Radtke AJ, et al. 2022. Nat Protoc. 17:378-401. (SB) PubMed

Product Citations

1. Jiang W, et al. 2022. Redox Biol. 56:102432. PubMed
2. Naranjo S, et al. 2022. Genes Dev. 36:936. PubMed
3. Yang H, et al. 2023. Cell Death Differ. 30:560. PubMed
4. Pennitz P 2022. STAR Protocols. 4(1):101957. PubMed
5. Kimura S, et al. 2020. Nat Commun. 0.620833333. PubMed
6. Gao Q, et al. 2019. J Exp Med. 216:688. PubMed
7. Wang M, et al. 2022. Theranostics. 12:4513. PubMed
8. Chiang HC, et al. 2018. Sci Rep. 8:2731. PubMed
9. Lancaster JN, et al. 2019. Nat Commun. 10:2220. PubMed
10. Nair S, et al. 2016. Nat Commun. 7:10913. PubMed
11. Schwartz AJ, et al. 2021. Nat Metab. 3:969. PubMed
12. Skibba ME, et al. 2021. Respir Res. 22:315. PubMed
13. Valds-Mora F, et al. 2021. Cell Reports. 35(2):108945. PubMed
14. Yin N et al. 2019. Cancer Cell. 36(2):156-167 . PubMed
15. Hao F, et al. 2021. Proc Natl Acad Sci U S A. 118:. PubMed
16. Yu D, et al. 2013. Mol Cell Biol. 33:1845. PubMed
17. Kim S, et al. 2011. PLoS One. 6:e19310. PubMed
18. Parker KA, et al. 2021. Mol Cancer Res. 19:2068. PubMed
19. Weiner AI, et al. 2019. NPJ Regen Med. 4:17. PubMed
20. Bahar Halpern K, et al. 2020. Nat Commun. 11:1936. PubMed
21. Airik M, et al. 2020. Hum Mol Genet. 29:3064. PubMed
22. Reinfeld BI, et al. 2021. Nature. 593:282. PubMed
23. Krueger A, et al. 2022. Front Microbiol. 12:789042. PubMed
24. Scur M, et al. 2022. Nat Commun. 13:7272. PubMed
25. Sun L, et al. 2015. Sci Rep. 5: 14871. PubMed
26. Lee J, et al. 2013. Am J Respir Cell Mol Biol. 48:288. PubMed
27. Tan C, et al. 2020. Front Cell Dev Biol. 8:579157. PubMed
28. Yang Y, et al. 2021. Cell Reports. 34(10):108822. PubMed
29. Mello AM, et al. 2022. Oncogenesis. 11:56. PubMed
30. Tateishi R, et al. 2015. PLoS One. 10: e0141650. PubMed
31. He B, et al. 2018. Nat Commun. 9:5240. PubMed
32. Zheng C, et al. 2022. Acta Pharm Sin B. 12:3726. PubMed
33. Li Q, et al. 2022. J Biol Chem. :101753. PubMed
34. Shigeyasu K, et al. 2015. Gut. 64:627. PubMed
35. Kudira R, et al. 2021. Bio Protoc. 11:e4192. PubMed
36. Fujino N, et al. 2019. J Exp Med. 216:2184. PubMed
37. Edelson B, et al. 2011. PLoS One. 6:e25660. PubMed
38. Barr J, et al. 2022. Elife. 11:. PubMed
39. Ichinose M, et al. 2021. Sci Rep. 7200:11. PubMed
40. Avgustinova A, et al. 2021. Cell Stem Cell. . PubMed
41. Pastor W, et al. 2014. Nat Commun. 12:5795. PubMed
42. Filbey KJ, et al. 2019. Front Immunol. 10:2375. PubMed

RRID

AB_1134172 (BioLegend Cat. No. 118205)
AB_1134172 (BioLegend Cat. No. 118206)

Antigen Details

Structure

40 kD single-pass type 1 glycoprotein. 293 amino acids, with a 21 aa signal peptide, a 246 aa extracellular domain, a 21 aa transmembrane domain, and a 26 aa cytoplasmic domain. The extracellular domain contains two epidermal growth factor-like repeats.

Distribution

Expressed on majority of epithelial cell membranes with the exception of adult squamous cells of the skin and a few specific epithelial cell types.

Function

Mediates calcium-independent homophilic cell-cell adhesion.

Interaction

CD326 displays hemophilic binding.

Ligand/Receptor

CD305 (LAIR-1), CD306 (LAIR-2), and Ep-CAM.

Cell Type

Embryonic Stem Cells, Epithelial cells

Biology Area

Immunology, Stem Cells

Molecular Family

Adhesion Molecules, CD Molecules

Antigen References

1. Borkowski TA, et al. 1996. Eur. J. Immunol. 26:110.
2. Bergsagel PL, et al. 1992. J. Immunol. 148:590.

Gene ID

17075 View all products for this Gene ID

Specificity (DOES NOT SHOW ON TDS):

CD326

Specificity Alt (DOES NOT SHOW ON TDS):

CD326

App Abbreviation (DOES NOT SHOW ON TDS):

FC

UniProt

View information about CD326 on UniProt.org

Documentation

• Technical Data Sheet (pdf)
• Certificate of Analysis
• Material Safety Data Sheet

Reviews

Related Protocols

• Cell Surface Flow Cytometry Staining Protocol

Related Pages & Pathways

Related FAQs

What type of PE do you use in your conjugates?

We use R-PE in our conjugates.

Other Formats

Certificate of Analysis