Spark Blue™ 550 anti-human CD8 Antibody, CD8a, SK1

Pricing & Availability

Clone: SK1 (See other available formats)
Regulatory Status: RUO
Other Names: T8, Leu2
Isotype: Mouse IgG1, κ
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Product Citations: publications

Human peripheral blood lymphocytes were stained with CD8 (clone SK1) Spark Blue™ 550 (filled histogram.) Open histogram represents unstained cells.

Compare all formats See Spark Blue™ 550 spectral data

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344759

25 tests

113€

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344760

100 tests

296€

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Description

CD8a is a 32-34 kD type I glycoprotein. It forms a homodimer (CD8a/a) or heterodimer (CD8a/b) with CD8b. CD8, also known as T8 and Leu2, is a member of the immunoglobulin superfamily found on the majority of thymocytes, a subset of peripheral blood T cells, and NK cells (which express almost exclusively CD8a homodimers). CD8 acts as a co-receptor with MHC class I-restricted T cell receptors in antigen recognition and T cell activation and has been shown to play a role in thymic differentiation. Two domains in CD8a are important for function: the extracellular IgSF domain binds the α₃ domain of MHC class I and the cytoplasmic CXCP motif binds the tyrosine kinase p56 Lck.

Product Details

Technical Data Sheet (pdf)

Product Details

Verified Reactivity: Human, Cynomolgus, Rhesus
Reported Reactivity: African Green, Chimpanzee, Pigtailed Macaque, Sooty Mangabey
Antibody Type: Monoclonal
Host Species: Mouse
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation: The antibody was purified by affinity chromatography and conjugated with Spark Blue™ 550 under optimal conditions.
Concentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.

* Spark Blue™ 550 has a maximum excitation of 516 nm and a maximum emission of 540 nm.
Excitation Laser: Blue Laser (488 nm)
Application Notes: Clone SK1 recognizes the a chain of CD8. Additional reported applications (for the relevant formats) include: proteogenomics⁸, immunohistochemistry of acetone-fixed frozen tissue sections, and spatial biology (IBEX)^9,10. This clone was tested in-house and does not demonstrate utility for formalin-fixed paraffin-embedded (FFPE) human tonsil sections.
RRID: AB_2819982 (BioLegend Cat. No. 344759)
AB_2819982 (BioLegend Cat. No. 344760)

Antigen Details

Structure: Ig superfamily, homodimer or heterodimer with CD8b, 32-34 kD
Distribution: Majority of thymocytes, T cell subset, NK cells
Function: MHC class I co-receptor, thymic differentiation, T cell activation
Ligand/Receptor: MHC Class I molecules
Cell Type: NK cells, T cells, Thymocytes
Biology Area: Immunology
Molecular Family: CD Molecules
Antigen References: 1. Barclay N, et al. 1993. The Leucocyte Antigen FactsBook. Academic Press Inc. San Diego.
Gene ID: 925 View all products for this Gene ID
Specificity (DOES NOT SHOW ON TDS):: CD8
Specificity Alt (DOES NOT SHOW ON TDS):: CD8
App Abbreviation (DOES NOT SHOW ON TDS):: FC
UniProt: View information about CD8 on UniProt.org

Documentation

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Spark Violet™ 500 anti-human CD8	SK1	FC
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Spark Violet™ 538 anti-human CD8	SK1	FC
PE/Fire™ 810 anti-human CD8	SK1	FC
Spark YG™ 593 anti-human CD8	SK1	FC

Certificate of Analysis

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Go To Top Version: 2 Revision Date: 09.14.2020

For Research Use Only. Not for diagnostic or therapeutic use.

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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