Spark Blue™ 550 anti-human CD8 Antibody, CD8a, SK1

Pricing & Availability

Clone: SK1 (See other available formats)
Regulatory Status: RUO
Other Names: T8, Leu2
Isotype: Mouse IgG1, κ
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Product Citations: publications

Human peripheral blood lymphocytes were stained with CD8 (clone SK1) Spark Blue™ 550 (filled histogram.) Open histogram represents unstained cells.

Compare all formats See Spark Blue™ 550 spectral data

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344759

25 tests

113€

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344760

100 tests

296€

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Description

CD8a is a 32-34 kD type I glycoprotein. It forms a homodimer (CD8a/a) or heterodimer (CD8a/b) with CD8b. CD8, also known as T8 and Leu2, is a member of the immunoglobulin superfamily found on the majority of thymocytes, a subset of peripheral blood T cells, and NK cells (which express almost exclusively CD8a homodimers). CD8 acts as a co-receptor with MHC class I-restricted T cell receptors in antigen recognition and T cell activation and has been shown to play a role in thymic differentiation. Two domains in CD8a are important for function: the extracellular IgSF domain binds the α₃ domain of MHC class I and the cytoplasmic CXCP motif binds the tyrosine kinase p56 Lck.

Product Details

Technical Data Sheet (pdf)

Product Details

Verified Reactivity: Human, Cynomolgus, Rhesus
Reported Reactivity: African Green, Chimpanzee, Pigtailed Macaque, Sooty Mangabey
Antibody Type: Monoclonal
Host Species: Mouse
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation: The antibody was purified by affinity chromatography and conjugated with Spark Blue™ 550 under optimal conditions.
Concentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.

* Spark Blue™ 550 has a maximum excitation of 516 nm and a maximum emission of 540 nm.
Excitation Laser: Blue Laser (488 nm)
Application Notes: Clone SK1 recognizes the a chain of CD8. Additional reported applications (for the relevant formats) include: proteogenomics⁸, immunohistochemistry of acetone-fixed frozen tissue sections, and spatial biology (IBEX)^9,10. This clone was tested in-house and does not demonstrate utility for formalin-fixed paraffin-embedded (FFPE) human tonsil sections.
RRID: AB_2819982 (BioLegend Cat. No. 344759)
AB_2819982 (BioLegend Cat. No. 344760)

Antigen Details

Structure: Ig superfamily, homodimer or heterodimer with CD8b, 32-34 kD
Distribution: Majority of thymocytes, T cell subset, NK cells
Function: MHC class I co-receptor, thymic differentiation, T cell activation
Ligand/Receptor: MHC Class I molecules
Cell Type: NK cells, T cells, Thymocytes
Biology Area: Immunology
Molecular Family: CD Molecules
Antigen References: 1. Barclay N, et al. 1993. The Leucocyte Antigen FactsBook. Academic Press Inc. San Diego.
Gene ID: 925 View all products for this Gene ID
Specificity (DOES NOT SHOW ON TDS):: CD8
Specificity Alt (DOES NOT SHOW ON TDS):: CD8
App Abbreviation (DOES NOT SHOW ON TDS):: FC
UniProt: View information about CD8 on UniProt.org

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Cell Surface Flow Cytometry Staining Protocol

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Brilliant Violet 750™ anti-human CD8	SK1	FC
TotalSeq™-B0046 anti-human CD8	SK1	PG
Spark Blue™ 550 anti-human CD8	SK1	FC
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APC/Fire™ 810 anti-human CD8	SK1	FC
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PerCP/Cyanine5.5 anti-human CD8	SK1	FC
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Spark UV™ 387 anti-human CD8	SK1	FC
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Spark NIR™ 685 anti-human CD8	SK1	FC
KIRAVIA Blue 520™ anti-human CD8	SK1	FC
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GMP Pacific Blue™ anti-human CD8	SK1	FC
GMP PerCP anti-human CD8	SK1	FC
Spark Violet™ 500 anti-human CD8	SK1	FC
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Alexa Fluor® 660 anti-human CD8a	SK1	FC
Spark Blue™ 515 anti-human CD8	SK1	FC
Spark Blue™ 574 anti-human CD8	SK1	FC
Spark Violet™ 538 anti-human CD8	SK1	FC
PE/Fire™ 810 anti-human CD8	SK1	FC
Spark YG™ 593 anti-human CD8	SK1	FC

Certificate of Analysis

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Go To Top Version: 2 Revision Date: 09.14.2020

For Research Use Only. Not for diagnostic or therapeutic use.

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Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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