Ultra-LEAF™ Purified anti-human HLA-A,B,C Antibody

Product Details

Verified Reactivity

Human, Cynomolgus, Rhesus

Reported Reactivity

African Green, Baboon, Cat, Cow, Chimpanzee

Antibody Type

Monoclonal

Host Species

Mouse

Formulation

0.2 µm filtered in phosphate-buffered solution, pH 7.2, containing no preservative.

Endotoxin Level

Less than 0.01 EU/µg of the protein (< 0.001 ng/µg of the protein) as determined by the LAL test.

Preparation

The Ultra-LEAF™ (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.

Concentration

The antibody is bottled at the concentration indicated on the vial, typically between 2 mg/mL and 3 mg/mL. Older lots may have also been bottled at 1 mg/mL. To obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.

Storage & Handling

The antibody solution should be stored undiluted between 2°C and 8°C. This Ultra-LEAF™ solution contains no preservative; handle under aseptic conditions.

Application

FC - Quality tested
Activ, Block, IHC-F, IP, WB - Reported in the literature, not verified in house

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤2.0 µg per million cells in 100 µl volume or 100 µl of whole blood.  It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Clone W6/32 recognizes residues in the N terminus of the human ß2-microglobulin molecule²¹.

Additional reported applications (for the relevant formats) include: immunoprecipitaton², Western blotting (non-reducing)³, immunohistochemical staining of acetone-fixed frozen tissue sections^4,5, blocking^6,7, inhibition of NK cell-mediated lysis¹⁰, and activation^8,9. Clone W6/32 has been reported not to be suitable for immunohistochemistry on paraffin sections¹⁷. The LEAF™ purified antibody (Endotoxin < 0.1 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays. For highly sensitive assays, we recommend Ultra-LEAF™ purified antibody (Cat. No. 311428) with a lower endotoxin limit than standard LEAF™ purified antibodies (Endotoxin < 0.01 EU/µg).

Application References

1. Darrow TL, et al. 1989. J. Immunol. 142:3329.
2. Stern P, et al. 1987. J. Immunol. 138:1088.
3. Tran TM, et al. 2001. Immunogenetics 53:440.
4. Barbatis C, et al. 1981. Gut 22:985.
5. Ayyoub M, et al. 2004. Cancer Immunity 4:7.
6. DeFelice M, et al. 1990. Cell. Immunol. 126:420.
7. Fayen J, et al. 1998. Int. Immunol. 10:1347.
8. Turco MC, et al. 1988. J. Immunol. 141:2275.
9. Geppert TD, et al. 1989. J. Immunol. 142:3763.
10. Wooden SL, et al. 2005. J. Immunol. 175:1383.
11. Nagano M, et al. 2007. Blood 110:151.
12. McLoughlin RM,et al.2008. J. Immunol. 181:1323. PubMed
13. Takahara M, et al.2008. J. Leukoc. Biol. 83:742. PubMed
14. Lunemann A, et al.2008. J. Immunol. 181:6170. PubMed
15. Laing BJ, et al. 2010. J. Thorac Cardiovasc Surg. 139:1402. PubMed
16. Yoshino N, et al. 2000. Exp. Anim. (Tokyo) 49:97. (FC)
17. Vambutas A, et al. 2000. Clin. Diagn. Lab. Immun. 7:79.
18. Coppieters KT, et al. 2012. J. Exp. Med. 209:51. (epitope)
19. Crivello P, et al. 2013. Hum Immunol. 22:100. PubMed
20. Jung Y, et al. 2015. Mol Cancer Res. 13:197. PubMed
21. Shields MJ. Ribaudo RK. 1998. Tissue Antigens. 51(5):567-70. (epitope)

Product Citations

1. Muik A, et al. 2022. Cancer Discov. 12:1248. PubMed
2. Lin W, et al. 2023. Commun Biol. 6:447. PubMed
3. Blandine Monel et al. 2019. Cell reports. 27(1):142-153 . PubMed
4. Baleeiro RB, et al. 2022. Oncoimmunology. 11:2080329. PubMed
5. Jensen SM, et al. 2018. Front Immunol. 9:2697. PubMed
6. Ito T, et al. 2021. Commun Biol. 4:694. PubMed
7. Arakawa A, et al. 2021. J Immunol. 207:2235. PubMed
8. Dangaj D, et al. 2019. Cancer Cell. 35:885. PubMed
9. Eleftheriadis T, et al. 2021. Mol Med Rep. :23. PubMed
10. Meng Q, et al. 2021. J Immunother Cancer. 9:. PubMed

RRID

AB_2561492 (BioLegend Cat. No. 311442)
AB_2561492 (BioLegend Cat. No. 311447)
AB_2561492 (BioLegend Cat. No. 311448)
AB_2561492 (BioLegend Cat. No. 311427)
AB_2561492 (BioLegend Cat. No. 311428)
AB_2561492 (BioLegend Cat. No. 311441)

Antigen Details

Structure

Ig superfamily

Distribution

All nucleated cells

Function

Antigen presentation

Ligand/Receptor

CD3/TCR, CD8

Biology Area

Immunology, Innate Immunity

Molecular Family

MHC Antigens

Antigen References

1. Barclay AN, et al. Eds. 1993. The Leukocyte Antigen FactsBook. Academic Press Inc. San Diego.

Gene ID

3105 View all products for this Gene ID

Specificity (DOES NOT SHOW ON TDS):

HLA-A

Specificity Alt (DOES NOT SHOW ON TDS):

HLA-A,B,C

App Abbreviation (DOES NOT SHOW ON TDS):

FC,Activ,Block,IHC-F,IP,WB

UniProt

View information about HLA-A on UniProt.org

Documentation

• Technical Data Sheet (pdf)
• Certificate of Analysis
• Material Safety Data Sheet

Reviews

Related Protocols

• Cell Surface Flow Cytometry Staining Protocol

Related Pages & Pathways

Related FAQs

Do you guarantee that your antibodies are totally pathogen free?

BioLegend does not test for pathogens in-house aside from the GoInVivo™ product line. However, upon request, this can be tested on a custom basis with an outside, independent laboratory.

Does BioLegend test each Ultra-LEAF™ antibody by functional assay?

No, BioLegend does not test Ultra-LEAF™ antibodies by functional assays unless otherwise indicated. Due to the possible complexities and variations of uses of biofunctional antibodies in different assays and because of the large product portfolio, BioLegend does not currently perform functional assays as a routine QC for the antibodies. However, we do provide references in which the antibodies were used for functional assays and we do perform QC to verify the specificity and quality of the antibody based on our strict specification criteria.

Does BioLegend test each Ultra-LEAF™ antibody for potential pathogens?

No, BioLegend does not test for pathogens in-house unless otherwise indicated.  However, we can recommend an outside vendor to perform this testing as needed.

Have you tested this Ultra-LEAF™ antibody for in vivo or in vitro applications?

We don't test our antibodies for in vivo or in vitro applications unless otherwise indicated. Depending on the product, the TDS may describe literature supporting usage of a particular product for bioassay. It may be best to further consult the literature to find clone specific information.

Other Formats

Certificate of Analysis