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Our large columns are optimized for use with MojoSort on Columns™ reagents. Our columns can obtain greater purity at a lower cost and display equivalent flow rates to competitor's equivalent columns. For more information, explore our column data below.

MojoSort on Columns™ Cell Separation System is BioLegend’s new portfolio of reagents optimized for column-based magnetic cell separation. Our cell separation columns contain an iron sphere matrix, which generate a strong magnetic field when placed on BioLegend’s Column, Separator, and Multistand. Our column system can be used for positive or negative selection of MojoSort on Columns™ kits and reagents. In negative selection, the untouched cells flow through the column and are collected directly into a tube. In positive selection, the labeled cells are retained in the column matrix and eluted by removing the column from the magnetic separator.

MojoSort on Columns™ reagents and kits are optimized for use on our columns, separators, and stands. They have also demonstrated compatibility with third party columns, column separators and stands. Please contact BioLegend Technical Service for more guidance on compatibility with third party systems.

Below, we show an example workflow for MojoSort on Columns™ Human CD3 T Cell Isolation Kit and Human CD4 T Cell Isolation Kit. For more information on kit-specific protocols please visit our protocols section.

(Top) Human CD4 T cells were isolated from fresh PBMCs using the MojoSort on Columns™ Human CD4 T Cell Isolation Kit with a BioLegend Large Column and Separator. For comparison, we simultaneously performed cell separation with a competitor’s equivalent column system and column kit for human CD4 T cells. The negatively selected cells were then stained with Spark Violet™ 538 anti-CD3 and APC anti-CD4. Then the cells were analyzed by flow cytometry on CD45⁺ live cells.

(Bottom) Untouched NK cells were isolated from frozen Leukopak-derived PBMCs using the MojoSort on Columns™ Human NK Cell Isolation Kit with BioLegend’s Large Columns and Separator. The negatively selected cells were then stained with APC anti-human CD3 and PE anti-human CD56. Then, the cells were analyzed by flow cytometry gated on live CD45⁺ cells.​

**Tested with buffer (PBS with 0.5% BSA).

*Cell capacity may vary depending on cell size.