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Alexa Fluor® 647 anti-HMOX1 Antibody

Pricing & Availability
Clone
W19398C (See other available formats)
Regulatory Status
RUO
Other Names
Heme Oxygenase 1, HO-1, Heat Shock Protein, 32-KD
Isotype
Rat IgG2a, κ
Ave. Rating
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Product Citations
publications
a. W19398C_Alexa-Fluor-647_ICFC_061323
HeLa cells untreated (low expression control, open histogram), or treated with CoCl2 (200 µM) (positive control, filled histogram) were fixed and permeabilized using Cyto-Fast™ Fix/Perm Buffer set (Cat. No. 426803) and intracellularly stained with Alexa Fluor® 647 anti-HMOX1 (clone W19398C), or Alexa Fluor® 647 rat IgG2a, κ isotype control (open histogram, dashed line) (representative for both untreated and treated HeLa cells) (Cat. No. 400526).
  • a. W19398C_Alexa-Fluor-647_ICFC_061323
    HeLa cells untreated (low expression control, open histogram), or treated with CoCl2 (200 µM) (positive control, filled histogram) were fixed and permeabilized using Cyto-Fast™ Fix/Perm Buffer set (Cat. No. 426803) and intracellularly stained with Alexa Fluor® 647 anti-HMOX1 (clone W19398C), or Alexa Fluor® 647 rat IgG2a, κ isotype control (open histogram, dashed line) (representative for both untreated and treated HeLa cells) (Cat. No. 400526).
  • b. W19398C_Alexa-Fluor-647_ICC_061323
    HeLa cells untreated (panels A and B) or treated with 200 µM of CoCl2 (panels C and D) were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with Triton X-100 for 10 minutes, and blocked with 5% FBS for 1 hour. Cells were then intracellularly stained with 2.5 µg/mL of Alexa Fluor® 647 rat IgG2a, κ isotype control (panels A and C) (Cat. No. 400526), or with 2.5 µg/mL of Alexa Fluor® 647 anti-HMOX1 (clone W19398C) (Red, panels B and D) overnight at 4°C. Nuclei were counterstained with DAPI (Cat. No. 422801), and actin was counterstained with Flash Phalloidin™ Green 488 (Cat. No. 424201). Images were captured with a 40X objective. Scale bar: 50 µm
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934105 25 tests 144€
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934106 100 tests 296€
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934108 100 µg 312€
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Description

HMOX1 is a member of the heme oxygenase family. Unlike its constitutive counterpart, it is an inducible enzyme triggered by external stimuli such as nitric oxide, growth factors, heavy metals, UV radiation, phorbol esters, inflammatory cytokines, heme, etc. Transcription of HMOX1 in tissue has been shown to be a product of the nrf2/ARE pathway where an increase in its protein levels results in heme degradation. The tissue protective effects of HMOX1 are a result of the bioactive products produced after heme degradation: iron ions, carbon monoxide, and biliverdin which is converted to bilirubin via biliverdin reductase.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Rat
Formulation
µg size: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide

test sizes: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 647 under optimal conditions.
Concentration
µg sizes: 0.5 mg/mL
test sizes: lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

ICFC (test sizes) - Quality tested
ICC (µg size) - Quality tested
Recommended Usage

Each lot of the test-sized antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining using the test sizes, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.

Each lot of the µg-sized antibody is quality control tested by immunocytochemistry. For immunocytochemistry using the ug size, a concentration range of 1.25 - 5.0 μg/mL is recommended. It is recommended that the reagent be titrated for optimal performance for each application. 

* Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633 nm / 635 nm.


Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.

View full statement regarding label licenses
Excitation Laser
Red Laser (633 nm)
Application Notes

Clone was tested for ICC using three fix/perm methods (PFA/Triton-X, PFA/MeOH, MeOH only), the best method was the use of PFA/Triton X-100.

In the CoCl2 treated cells for ICC, staining was seen in less than 5% of cells.

Clone was tested for ICFC using cytokine fix/perm method only (based on ICC results). 

 

RRID
AB_2941637 (BioLegend Cat. No. 934105)
AB_2941637 (BioLegend Cat. No. 934106)
AB_2941637 (BioLegend Cat. No. 934108)

Antigen Details

Distribution

Cytosol/Endoplasmic reticulum

Biology Area
Cell Biology
Molecular Family
Enzymes and Regulators
Antigen References

1. Yang B, et al. 2018. Toxicol Appl Pharmacol. 355:189-197
2. Chora A, et al. 2007. J Clin Invest. 117(2):438-47
3. Harding H, et al. 2003. Mol Cell. 11(3):619-33.

Gene ID
3162 View all products for this Gene ID
UniProt
View information about HMOX1 on UniProt.org
Go To Top Version: 1    Revision Date: 06.13.2023

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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