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Alexa Fluor® 647 anti-human CD101 (BB27) Antibody

Pricing & Availability
Clone
BB27 (See other available formats)
Regulatory Status
RUO
Workshop
V T040
Other Names
V7, P126
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
BB27_AF647_CD101_Antibody_FC_1_051613
Human peripheral blood lymphocytes were stained with CD3 PE and CD101 (clone BB27) Alexa Fluor® 647 (top) or mouse IgG1, κ Alexa Fluor® 647 isotype control (bottom).
  • BB27_AF647_CD101_Antibody_FC_1_051613
    Human peripheral blood lymphocytes were stained with CD3 PE and CD101 (clone BB27) Alexa Fluor® 647 (top) or mouse IgG1, κ Alexa Fluor® 647 isotype control (bottom).
  • BB27_AF647_CD101_Antibody_FC_1_051613
Compare all formats See Alexa Fluor® 647 spectral data
Cat # Size Price Quantity Check Availability Save
331009 25 tests 128€
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331010 100 tests 287€
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Description

CD101 is a type I transmembrane glycoprotein, known as V7, and P126. It is a 120 kD homodimer, the member of EWI family within Ig superfamily. CD101 is found on monocytes, granulocytes, dendritic cells, a subpopulation of peripheral blood T cells and activated T cells. It has been reported that CD101 expressing Tregs posses higher inhibitory function. The biological function of CD101 is still not clear. But it has been found that BB27 antibody is able to inhibit T cell proliferative responses and this inhibitory function can be overcome by high doses of IL-2. Activation of CD101 on dendritic cells is able to induce IL-10 production.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Reported Reactivity
African Green, Baboon, Cynomolgus, Rhesus
Antibody Type
Monoclonal
Host Species
Mouse
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 647 under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

* Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633 nm / 635 nm.


Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.

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Excitation Laser
Red Laser (633 nm)
Application References
  1. Gouttefangeas C, et al. 1994. Int. Immunol. 6:423
  2. Bouloc A, et al. 2000. Histopathology. 36:229
Product Citations
  1. Silvin A, et al. 2020. Cell. 182:1401. PubMed
  2. , et al. 2021. Eur J Immunol. 51:2708. PubMed
RRID
AB_2562144 (BioLegend Cat. No. 331009)
AB_2562144 (BioLegend Cat. No. 331010)

Antigen Details

Structure
Type I transmembrane glycoprotein, homodimer, Ig superfamily, 120 kD
Distribution

Monocytes, granulocytes, dendritic cells, T cell subset and activated T cells

Cell Type
Dendritic cells, Granulocytes, Monocytes, T cells
Biology Area
Immunology
Antigen References

1. Zola H, et al. Eds. 2007. Leukocyte and Stromal Cell Molecules:The CD Markers. A John Wiley & Sons Inc, Publication
2. Bagot M, et al. 1997. Tissue Antigen 50:439

Gene ID
9398 View all products for this Gene ID
UniProt
View information about CD101 on UniProt.org
Go To Top Version: 2    Revision Date: 06.27.2014

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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