Alexa Fluor 647 anti-human CD101 BB27 Antibody anti-CD101

Pricing & Availability

Clone: BB27 (See other available formats)
Regulatory Status: RUO
Workshop: V T040
Other Names: V7, P126
Isotype: Mouse IgG1, κ
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Product Citations: publications

BB27_AF647_CD101_Antibody_FC_1_051613 — Human peripheral blood lymphocytes were stained with CD3 PE and CD101 (clone BB27) Alexa Fluor® 647 (top) or mouse IgG1, κ Alexa Fluor® 647 isotype control (bottom).

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331009

25 tests

128€

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331010

100 tests

287€

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Description

CD101 is a type I transmembrane glycoprotein, known as V7, and P126. It is a 120 kD homodimer, the member of EWI family within Ig superfamily. CD101 is found on monocytes, granulocytes, dendritic cells, a subpopulation of peripheral blood T cells and activated T cells. It has been reported that CD101 expressing Tregs posses higher inhibitory function. The biological function of CD101 is still not clear. But it has been found that BB27 antibody is able to inhibit T cell proliferative responses and this inhibitory function can be overcome by high doses of IL-2. Activation of CD101 on dendritic cells is able to induce IL-10 production.

Product Details

Technical Data Sheet (pdf)

Product Details

Verified Reactivity: Human
Reported Reactivity: African Green, Baboon, Cynomolgus, Rhesus
Antibody Type: Monoclonal
Host Species: Mouse
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation: The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 647 under optimal conditions.
Concentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

* Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633 nm / 635 nm.

Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.

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Excitation Laser: Red Laser (633 nm)
RRID: AB_2562144 (BioLegend Cat. No. 331009)
AB_2562144 (BioLegend Cat. No. 331010)

Antigen Details

Structure: Type I transmembrane glycoprotein, homodimer, Ig superfamily, 120 kD
Distribution: Monocytes, granulocytes, dendritic cells, T cell subset and activated T cells
Cell Type: Dendritic cells, Granulocytes, Monocytes, T cells
Biology Area: Immunology
Antigen References: 1. Zola H, et al. Eds. 2007. Leukocyte and Stromal Cell Molecules:The CD Markers. A John Wiley & Sons Inc, Publication
2. Bagot M, et al. 1997. Tissue Antigen 50:439
Gene ID: 9398 View all products for this Gene ID
UniProt: View information about CD101 on UniProt.org

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Related Protocols

Cell Surface Flow Cytometry Staining Protocol

Related Products

Description	Clone	Applications

Related Pages & Pathways

Pathways

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Other Formats

View All CD101 Reagents Request Custom Conjugation

Description	Clone	Applications
APC anti-human CD101 (BB27)	BB27	FC
Alexa Fluor® 647 anti-human CD101 (BB27)	BB27	FC
PE/Cyanine7 anti-human CD101 (BB27)	BB27	FC
PerCP/Cyanine5.5 anti-human CD101 (BB27)	BB27	FC
PE anti-human CD101 (BB27)	BB27	FC
TotalSeq™-C0944 anti-human CD101 (BB27)	BB27	PG
TotalSeq™-B0944 anti-human CD101 (BB27) Antibody	BB27	PG
TotalSeq™-A0944 anti-human CD101 (BB27)	BB27	PG

Certificate of Analysis

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Go To Top Version: 2 Revision Date: 06.27.2014

For Research Use Only. Not for diagnostic or therapeutic use.

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

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