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Alexa Fluor® 647 anti-human CD169 (Sialoadhesin, Siglec-1) Antibody

Pricing & Availability
Clone
7-239 (See other available formats)
Regulatory Status
RUO
Other Names
Sialic acid binding Ig-like lectin 1 (Siglec-1)
Isotype
Mouse IgG1, κ
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Product Citations
publications
7-239_AF647_1_040111
Human peripheral blood monocytes were stimulated with TNF-α plus IFN-γ for 3 days, then stained with CD14 FITC and CD169 (clone 7-239) Alexa Fluor® 647 (top) or mouse IgG1, κ Alexa Fluor® 647 isotype control (bottom).
  • 7-239_AF647_1_040111
    Human peripheral blood monocytes were stimulated with TNF-α plus IFN-γ for 3 days, then stained with CD14 FITC and CD169 (clone 7-239) Alexa Fluor® 647 (top) or mouse IgG1, κ Alexa Fluor® 647 isotype control (bottom).
  • 7-239_AF647_2_040111
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346005 25 tests 109€
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346006 100 tests 239€
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Description

CD169, also known as Siglec-1 and Sialoadhesin (Sn), is a 210 kD type I single membrane-spanning glycoprotein. It is the largest member of the Siglec family, consisting of 1709 amino acids and belonging to the immunoglobulin superfamily. CD169 is expressed by macrophages and dendritic cells. By its affinity to α2,3-linked sialic acid, it is involved in macrophage binding to different cell types such as granulocytes, monocytes, NK, B and T cells. Several CD169 counter receptors, such as CD227 on human breast cancer cells, CD43 on T cells and CD206 on macrophages, have been reported.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Human Rhinovirus (HRV14) infected, monocyte derived-DCs
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 647 under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

* Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633 nm / 635 nm.


Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.

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Excitation Laser
Red Laser (633 nm)
Application Notes

Additional reported applications (for the relevant formats) include: Western blotting and inhibition of erythrocyte-rosetting with cells expressing CD169.

Application References
  1. Kirchberger S, et al. 2005. J. Immunol. 175:1145.
  2. Schrauf C, et al. 2009. J. Immunol. 183:4440.
Product Citations
  1. Preglej T, et al. 2023. Front Immunol. 14:1096096. PubMed
  2. Jalloh S, et al. 2022. PLoS Pathog. 18:e1010479. PubMed
  3. Li C, et al. 2015. PLoS One. 10: e0141817. PubMed
  4. Rose T, et al. 2016. RMD Open. 2: e000292. PubMed
RRID
AB_10897945 (BioLegend Cat. No. 346005)
AB_10897945 (BioLegend Cat. No. 346006)

Antigen Details

Structure
Type I single membrane-spanning lectin, 1709 amino acids, 210 kD, belongs to the immunoglobulin superfamily
Distribution

Macrophages, dendritic cells

Function
Adhesion molecule
Interaction
α-2,3-linked sialic acid
Ligand/Receptor
CD227, CD43 and CD206
Bioactivity
Mediates sialic-acid dependent binding to granulocytes, monocytes, NK, B and T cells.
Cell Type
B cells, Dendritic cells, Macrophages
Biology Area
Cell Adhesion, Cell Biology, Immunology, Innate Immunity
Molecular Family
Adhesion Molecules, CD Molecules, Siglec Molecules
Antigen References

1. Xiong YS, et al. 2009. Clin. Biochem. 42:1057.
2. Varki A, et al. 2009. Glycoconj J. 26:231.
3. Rempel H, et al. 2008. PLoS One. 3:e1967.
4. Crocker PR, et al. 2001. Trends Immunol. 22:337.
5. Hartnell A, et al. 2001. Blood 97:288.

Gene ID
6614 View all products for this Gene ID
UniProt
View information about CD169 on UniProt.org
Go To Top Version: 2    Revision Date: 06.27.2014

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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