- Clone
- 1-21 (See other available formats)
- Regulatory Status
- RUO
- Other Names
- T-cell leukemia/lymphoma protein 1A, Protein p14 TCL1, Oncogene TCL1, TCL-1
- Isotype
- Mouse IgG2b, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
Cat # | Size | Price | Quantity Check Availability | Save | ||
---|---|---|---|---|---|---|
330508 | 100 tests | 247€ |
TCL1, also known as T-cell leukemia/lymphoma protein 1A, protein p14 TCL1 and oncogene TCL-1, is a 14kD beta-barrel protein that was originally identified on the basis of its association with T-cell prolymphocytic leukemia (T-PLL). Activated by the T-cell receptor enhancer through inv(14)(q11;q32.1) or t(14;14)(q11;q32.1), TCL1 is normally expressed in immature cortical thymocytes, activated peripheral T-cells, pro-B cells, and naive mantle zone B cells of peripheral lymphoid tissues, but not in post-germinal center (GC) memory B cells and plasma cells. Akt and TCL1 interact via Akt’s pleckstrin homology domain to form hetero-oligomers at the leaflet of the inner plasma membrane stimulating the protein kinase C-mitogen activated protein kinase-extracellular signal-related kinase (PKC-MAPK-ERK) signal transduction pathway. TCL1 also inhibits activation-induced cell death in T-cells by blocking PKCθ. In addition to T-PLL dysregulation is implicated in such B-cell neoplasias as lymphoblastic lymphoma, chronic lymphocytic leukemia (CLL) and Burkitt lymphoma.
Product DetailsProduct Details
- Verified Reactivity
- Human
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- outer α-loop region of TCL1 peptide
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
- Preparation
- The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 647 under optimal conditions.
- Concentration
- Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
ICFC - Quality tested
- Recommended Usage
-
Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.
* Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633nm / 635nm.
Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.
View full statement regarding label licenses - Excitation Laser
-
Red Laser (633 nm)
- Application Notes
-
Additional reported applications include: immunoprecipitation, western blot, immunohistochemical staining of frozen or formalin-fixed, paraffin embedded tissue sections.
- Application References
-
- Herling M, et al. 2008. Blood 111:328
- Herling M, et al. 2007. Am. J. Surg. Pathol. 31:1123
- Product Citations
-
- RRID
-
AB_2204405 (BioLegend Cat. No. 330508)
Antigen Details
- Structure
- Beta barrel protein, 14kD
- Distribution
- Cytoplasm
- Function
- Enhances Akt mediated cell survival, stabilizes mitochondrial membrane potential
- Interaction
- Akt1, Akt2, Akt3, caco2
- Biology Area
- Cell Biology, Immunology
- Molecular Family
- CD Molecules
- Antigen References
-
1. Narducci MG et al. 2000. Cancer Research 60:2095.
2. Teitell MA et al. 2005 Nat. Rev. Cancer 5:640
3. Laine J et al.2000. Molecular Cell 6:395
4. Virgilio L et al. 1994. Proc. Natl. Acad. Sci. 91:12530
5. Masayuk N et al. 2007 FASEB J. 21:2273
6. Despouy G et al. 2007 Blood 110:4406
7. Rhine S et al. 2006 Blood 108:1991 - Gene ID
- 8115 View all products for this Gene ID
- UniProt
- View information about TCL1 on UniProt.org
Other Formats
View All TCL1 Reagents Request Custom ConjugationDescription | Clone | Applications |
---|---|---|
Alexa Fluor® 647 anti-human TCL1 | 1-21 | ICFC |
PE anti-human TCL1 | 1-21 | ICFC |
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Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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Alexa Fluor® 647 anti-human TCL1
Human peripheral blood lymphocytes stained with anti-CD19 (H... -
PE anti-human TCL1
Human peripheral blood lymphocytes stained with anti-CD19 (H...
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