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Alexa Fluor® 647 anti-mouse TCR Vα2 Antibody

Pricing & Availability
Clone
B20.1 (See other available formats)
Regulatory Status
RUO
Other Names
T cell receptor Vα 2
Isotype
Rat IgG2a, λ
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Product Citations
publications
B20dot1_A647_090808.jpg
BALB/c splenocytes stained with B20.1 Alexa Fluor® 647
  • B20dot1_A647_090808.jpg
    BALB/c splenocytes stained with B20.1 Alexa Fluor® 647
Compare all formats See Alexa Fluor® 647 spectral data
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127812 100 µg 184€
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Description

The TCR alpha (α) chain complexes with the TCR beta (β) chain to form the T cell receptor in 95% of T cells, whereas the remaining 5% of T cells express gamma and delta chains (γ/δ).  TCR Vα2 is a distinct TCR subfamily found in mice having the a, b, and c haplotypes.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Soluble TCR from mouse CTL clone KB5-C20
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 647 under optimal conditions.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

* Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633nm / 635nm.


Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.

View full statement regarding label licenses
Excitation Laser
Red Laser (633 nm)
Application Notes

The B20.1 antibody recognizes most members of the Va2 TCR subfamily in mice having the a, b, and c haplotypes.

Application References
  1. Pircher H, et al. 1992. Eur. J. Immunol.. 22:399.
  2. Gregoire C, et al. 1991. P. Natl. Acad. Sci. USA 88:8077.
  3. Kao C, et al. 2005. Int. Immunol.17:1607. PubMed
  4. Steptoe RJ, et al. 2007. J. Immunol. 178:2094. PubMed
  5. Rao RR, et al. 2012. Immunity. 36:374. PubMed.
Product Citations
  1. Mier-Aguilar C, et al. 2016. PLoS One. 11:e0168155. PubMed
  2. Allen G 2009. J Immunol. 182:6639. PubMed
  3. Waldman MM, et al. 2022. Front Immunol. 13:856977. PubMed
RRID
AB_1186115 (BioLegend Cat. No. 127812)

Antigen Details

Distribution

On a subset of T cells

Function
In combination of V β, Vα recognizes MHC/peptide complex on the antigen-presenting cells.
Interaction
Associate with Vβ and CD3 complex to form TCR complexes expressing on cell surface.
Ligand/Receptor
TCR recognizes MHC/peptide complex
Cell Type
T cells
Biology Area
Adaptive Immunity, Immunology
Molecular Family
TCRs
Antigen References

1. Kubo RT, et al. 1989. J. Immunol.. 142:2736.
2. Pircher H, et al. 1992. Eur. J. Immunol. 22:399.

Gene ID
21473 View all products for this Gene ID
UniProt
View information about TCR Valpha2 on UniProt.org
Go To Top Version: 2    Revision Date: 06.27.2014

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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