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Alexa Fluor® 647 anti-Nos2 (iNOS) Antibody

Pricing & Availability
Clone
W16030C (See other available formats)
Regulatory Status
RUO
Other Names
Nitric oxide synthase 2, nitric oxide synthase 2A, NOS2A, HEP-NOS, inducible nitric oxide synthase (iNOS), Nitric oxide synthase, inducible, Inducible NOS
Isotype
Rat IgG2b, κ
Ave. Rating
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Product Citations
publications
W16030C_A647_Nos2_Antibody_030124
Thioglycolate-elicited BALB/c mouse peritoneal macrophages were incubated overnight with (left) and without (right) LPS. Cells were then surface stained with anti-mouse/human CD11b (clone M1/70) FITC before being fixed with Fixation Buffer and permeabilized with Intracellular Staining Permeabilization Wash Buffer. Finally, cells were intracellularly stained with anti-Nos2 (iNOS) (clone W16030C) Alexa Fluor® 647.
  • W16030C_A647_Nos2_Antibody_030124
    Thioglycolate-elicited BALB/c mouse peritoneal macrophages were incubated overnight with (left) and without (right) LPS. Cells were then surface stained with anti-mouse/human CD11b (clone M1/70) FITC before being fixed with Fixation Buffer and permeabilized with Intracellular Staining Permeabilization Wash Buffer. Finally, cells were intracellularly stained with anti-Nos2 (iNOS) (clone W16030C) Alexa Fluor® 647.
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696811 25 µg 128€
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696812 100 µg 316€
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Description

Nitric Oxide Synthase 2 (NOS2), also known as inducible NOS (iNOS), contains an N-terminal oxygenase domain and a C-terminal reductase domain, and functions to catalyze the formation of nitric oxide (NO) from L-arginine. NO is a reactive free radical which acts as a biologic messenger with diverse functions throughout the body, such as neurotransmission, antimicrobial, and antitumor activity. NOS2 is involved in inflammatory responses and enhances the synthesis of PGE2 and proinflammatory cytokines such as IL-6 and IL-8. The NOS2 gene is highly expressed in liver and is inducible by a combination of bacterial endotoxins and certain cytokines, including IL-1, IFNγ and TNFα. NOS2 also has nitrosylase activity and mediates cysteine S-nitrosylation of cytoplasmic target proteins such as COX2.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Partial mouse NOS2 recombinant protein (1-250 a.a.) expressed in E. coli.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation
The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 647 under optimal conditions.
Concentration
0.5 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

ICFC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.

* Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633 nm / 635 nm.


Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.

View full statement regarding label licenses
Excitation Laser
Red Laser (633 nm)
Application Notes

Mouse NOS2 has two isoforms: Isoform A is 130 kD and isoform B is 118 kD. This clone recognizes isoform B better than A in the tested cell.

RRID
AB_3106058 (BioLegend Cat. No. 696811)
AB_3106058 (BioLegend Cat. No. 696812)

Antigen Details

Structure
1144 amino acids with a predicted molecular weight of 130 kD. Contains a flavodoxin-like domain and a FAD-binding FR-type domain.
Distribution

Cytoplasm

Function
NOS2 is a nitric oxide synthase and produces large quantity of NO in response to the stimulation of proinflammatory cytokines. NOS2 plays an important role in host defense against pathogens and participates in anti-tumor processes.
Interaction
Forms a homodimer. Interacts with SLC9A3R1, GAPDH, S100A8, and S100A9.
Biology Area
Cell Biology, Immunology, Innate Immunity
Antigen References

1. Salim T, et al. 2016. PLoS One. 11:e0153289.
2. Ma Z, et al. 2016. Inflammation. 39:543.
3. Lee SH, et al. 2016. J. Cell. Biochem. 117:978.
4. Landes MB, et al. 2015. J. Leuko. Biol. 97:1111.
5. Karpuzoglu E, et al. 2006. Nitric. Oxide 15:177.
6. Suschek CV, et al. 2004. Curr. Mol. Med. 4:763.

Gene ID
4843 View all products for this Gene ID
UniProt
View information about Nos2 on UniProt.org
Go To Top Version: 1    Revision Date: 03.01.2024

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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