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Alexa Fluor® 700 anti-mouse Ig light chain κ Antibody

Pricing & Availability
Clone
RMK-45 (See other available formats)
Regulatory Status
RUO
Other Names
Immunoglobulin light chain kappa
Isotype
Rat IgG
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Product Citations
publications
RMK-45_AF700_IgK_Antibody_FC_061714
C57BL/6 splenocytes were stained with anti-mouse Ig light chain κ (clone RMK-45) Alexa Fluor® 700 (filled histogram) or rat IgG1, κ Alexa Fluor® 700 isotype control (open histogram).
  • RMK-45_AF700_IgK_Antibody_FC_061714
    C57BL/6 splenocytes were stained with anti-mouse Ig light chain κ (clone RMK-45) Alexa Fluor® 700 (filled histogram) or rat IgG1, κ Alexa Fluor® 700 isotype control (open histogram).
  • RMK-45_AF700_IgK_Antibody_FC_2_061714
    Human peripheral blood lymphocytes were stained with (filled histogram) or without (open histogram) purified mouse IgG1, κ ant-human CD3 (clone UCHT1), followed by anti-mouse Ig light chain κ (clone RMK-45) Alexa Fluor® 700.
Compare all formats See Alexa Fluor® 700 spectral data
Cat # Size Price Quantity Check Availability Save
409507 25 µg 100€
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409508 100 µg 259€
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Description

The RMK-45 monoclonal antibody reacts with immunoglobulin light chain kappa in all tested mouse haplotype (Igh-a and b). It does not react with the lambda chain.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 700 under optimal conditions.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.5 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

* Alexa Fluor® 700 has a maximum emission of 719 nm when it is excited at 633 nm / 635 nm. Prior to using Alexa Fluor® 700 conjugate for flow cytometric analysis, please verify your flow cytometer's capability of exciting and detecting the fluorochrome.


Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.

View full statement regarding label licenses
Excitation Laser
Red Laser (633 nm)
Application Notes

The RMK-12 monoclonal antibody may be used as a primary antibody for ELISA or as secondary for detection of mouse Ig kappa.

Product Citations
  1. Webster P, et al. 2018. Nat Commun. 9:2649. PubMed
RRID
AB_2563582 (BioLegend Cat. No. 409507)
AB_2563582 (BioLegend Cat. No. 409508)

Antigen Details

Structure
Immunoglobulin family
Biology Area
Apoptosis/Tumor Suppressors/Cell Death, Cell Biology, Protein Synthesis
Antigen References

1. Ludwig TE, et al. 2006. Nat. Methods. 3:637.
2. Nguyen DH, et al. 2005. J. Immunol. 175:228.
3. Bardor M, et al. 2005. J. Biol. Chem. 280:4228.
4. Diaz SL, et al. 2009. PLoS One. 4:e4241.

Gene ID
243469 View all products for this Gene ID
UniProt
View information about Ig light chain kappa on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 2    Revision Date: 06.27.2014

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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