- Clone
- P2B1 (See other available formats)
- Regulatory Status
- RUO
- Other Names
- Platelet endothelial cell adhesion molecule, PECA1, GPIIA', endoCAM, CD31 antigen, CD31/EndoCAM, adhesion molecule, PECAM-1, CD31/EndoCAM
- Previously
-
Covance Catalog# MMS-484R
- Isotype
- Mouse IgG1, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
Cat # | Size | Price | Quantity Check Availability | Save | ||
---|---|---|---|---|---|---|
910003 | 100 µL | 203€ |
PECAM (platelet endothelial cell adhesion molecule) is expressed on activated T-cells and endothelial cells and is also known as CD31. The protein is involved in transmigration of leukocytes across endothelium during inflammation. P2B1 activates the binding of human T-lymphocytes to endothelial cells.
Product DetailsProduct Details
- Verified Reactivity
- Human
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- The P2B1 monoclonal antibody was generated against IL-1β activated human umbilical vein endothelial cells (HUVECs).
- Preparation
- Ascites
- Concentration
- The concentration is not quantified as this product is sold as undiluted crude mouse ascites fluid. The concentration might vary from lot-to-lot and an estimated concentration would be 1-3 mg/ml.
- Storage & Handling
- Store at -20°C. Upon initial thawing, apportion into working aliquots and store at -20°C. Avoid repeated freeze-thaw cycles to prevent denaturing the antibody. For long-term storage, keep the antibody at -80°C.
- Application
-
IHC-F - Quality tested
ICC, WB, IP - Verified - Recommended Usage
-
Each lot of this antibody is quality control tested by immunohistochemical staining on frozen tissue sections. For immunohistochemistry, a dilution range of 1:500 - 1:1000 is suggested. For immunocytochemistry, a range of 1:250 - 1:500 is recommended. For Western blotting, the suggested use of this reagent is 1:1000 dilution. For immunoprecipitation, the suggested use of this reagent is 1:1000 dilution. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
-
This antibody is effective in immunoblotting, immunocytochemistry (ICC), immunoprecipitation (IP) and immunohistochemistry (IHC).
*Predicted MW = 83 kD
Positive Control: human kidney - Application References
-
- Brooks PC, et al. 1995. J Clin Invest. 96:1815. (IF)
- Product Citations
-
- RRID
-
AB_2565394 (BioLegend Cat. No. 910003)
Antigen Details
- Biology Area
- Cell Biology, Cell Motility/Cytoskeleton/Structure, Neuroinflammation, Neuroscience, Signal Transduction, Synaptic Biology, Transcription Factors
- Molecular Family
- Adhesion Molecules, CD Molecules
- Gene ID
- 5175 View all products for this Gene ID
- UniProt
- View information about CD31 on UniProt.org
Other Formats
View All CD31 (PECAM-1) Reagents Request Custom ConjugationDescription | Clone | Applications |
---|---|---|
Anti-CD31 (PECAM-1) | P2B1 | IHC-F,ICC,WB,IP |
Purified anti-CD31 (PECAM-1) | P2B1 | IHC-F,ICC |
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Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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