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Anti-E. coli RNA Polymerase β Antibody

Pricing & Availability
Clone
8RB13 (See other available formats)
Regulatory Status
RUO
Other Names
RNAP subunit beta, rpoB, groN, nitB, rif, ron, stl, stv, tabD, RNA polymerase subunit beta, Transcriptase subunit beta
Previously
NeoClone Catalog# W0023
Isotype
Mouse IgG2b, κ
Ave. Rating
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Product Citations
publications
1-8RB13_E.coliRNAPolymeraseBetaPrime_Antibody_WB_012419
Total cell lysates (15 µg total protein) from E. Coli BL21 cells were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to a nitrocellulose membrane, and probed with 0.01 µg/mL (1:100,000 dilution) of Purified anti-E. Coli RNA Polymerase β Antibody, clone 8RB13, overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP goat anti-mouse IgG Antibody (Cat. No. 405306) at a 1:3000 dilution. Lane M: Molecular Weight marker.
  • 1-8RB13_E.coliRNAPolymeraseBetaPrime_Antibody_WB_012419
    Total cell lysates (15 µg total protein) from E. Coli BL21 cells were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to a nitrocellulose membrane, and probed with 0.01 µg/mL (1:100,000 dilution) of Purified anti-E. Coli RNA Polymerase β Antibody, clone 8RB13, overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP goat anti-mouse IgG Antibody (Cat. No. 405306) at a 1:3000 dilution. Lane M: Molecular Weight marker.
  • 2-8RB13_E.coliRNAPolymeraseBetaPrime_Antibody_WB_092116
    Total cell lysates (15 µg protein) from E. Coli strain BL21 and HeLa were resolved by 4-12% Bis-tris gel electrophoresis, transferred to nitrocellulose, and probed with Anti-E. Coli RNA Polymerase β Antibody (clone 8RB13) followed by HRP goat anti-mouse IgG antibody and chemiluminescence detection.
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663903 100 µL 259€
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Description

RNA polymerase (RNAP or RNApol) is an enzyme that produces primary transcript RNA. The β subunit is the second-largest subunit, and is encoded by the roB gene. RNA polymerase β is a subunit which, along wtih α (rpoC), limits core polymerase expression.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
E. coli
Reported Reactivity
Other species
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Purified recombinant E. coli RNA polymerase β subunit.
Preparation
Dried ascites
Storage & Handling
Store lyophilized antibody at -20°C. Avoid repeat freeze/thaw cycles.
Application

WB - Quality tested
ELISA - Verified
IP - Reported in the literature, not verified in house
Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. To reconstitute, add 100 µl of ddH2O to the tube containing antibody and mix to dissolve. For Western blotting, the suggested dilution is 1:1000 after reconsitution. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Clone 8RB13 is extremely useful for detecting or purifying core RNAP from a broad range of bacteria. The purified polymerase is highly active for in vitro transcription.

Additional reported applications (for the relevant formats) include: immunoprecipitation3. The epitope of clone 8RB13 has been mapped to the sequence PEEKLLRAIFGEKAS3.

Application References
  1. Bergendahl V, et al. 2003. Protein Expr. Purif. 31:155.
  2. Burgess RR and Thompson NE. 2002. Curr. Opin. Biotechnol. 13:304.
  3. Stalder, ES. et al. 2011. Protein Expr. Purif. 77(1):26-33. (Epitope, ELISA, IP, WB)
Product Citations
  1. Gibson JA, et al. 2022. Proc Natl Acad Sci U S A. 119:e2210115119. PubMed
  2. Heinrich K, et al. 2019. MBio. 10. PubMed
  3. Ju X, et al. 2019. Nat Microbiol. 1.490972222. PubMed
  4. Broder U, Jaeger T, Jenal U 2016. Nat Microbiol. 2:16184. PubMed
  5. Ting SY, et al. 2020. Cell Host Microbe. 28(2):313-321.e6. PubMed
  6. Ting SY, et al. 2022. Elife. 11:. PubMed
  7. Kouba T, et al. 2020. Nat Commun. 11:6419. PubMed
  8. Stringer AM, et al. 2020. Elife. 9:00. PubMed
  9. Icke C, et al. 2021. eLife. 10:00. PubMed
  10. Osuna BA, et al. 2020. Cell Host Microbe. 28:31. PubMed
  11. Pribis JP et al. 2019. Mol Cell. 74(4):785-800 . PubMed
RRID
AB_2564524 (BioLegend Cat. No. 663903)

Antigen Details

Structure
1252 amino acids with a predicted molecular weight of approximately 150 kD.
Distribution

Bacteria.

Function
Transcriptional regulator in bacteria.
Biology Area
Cell Biology, Transcription Factors
Molecular Family
Nuclear Markers
Antigen References

1. Thompson NE, et al. 1992. Biochemistry 31:7003.
2. Jerald Hurwitz, et al. 2005. J. Biol. Chem. 280:42477.
3. Hirata A, et al. 2008. Nature. 451:851.
4. Bergendahl IV, et al. 2003. Protein Expr. Purif. 31:155.
5. Burgess RR and Thompson NE. 2002. Curr. Opin. Biotechnol. 13:304.

Gene ID
948488 View all products for this Gene ID
UniProt
View information about RNA Polymerase beta on UniProt.org
Go To Top Version: 5    Revision Date: 09.19.2023

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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