Anti-Neurofilament H & M NF-H/NF-M, Hypophosphorylated Antibody anti-Neurofilament H & M (NF-H/NF-M), Phospho

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Clone: SMI 35 (See other available formats)
Regulatory Status: RUO
Other Names: Neurofilament heavy polypeptide, NF-H, 200 kD neurofilament protein, neurofilament triplet H protein
Previously: Covance Catalog# SMI-35R
Isotype: Mouse IgG1, κ
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Product Citations: publications

SMI-35_Purified_NF-H_Antibody_1_101618 — IHC staining of Neurofilament H & M (NF-H/NF-M), Hypophosphorylated antibody (clone SMI 35) on formalin-fixed paraffin-embedded mouse brain tissue. Following antigen retrieval using Retrieve-All Antigen Unmasking System 3: Acidic, 100X (Cat. No. 927601), the tissue was incubated with a 1:2000 dilution of the primary antibody overnight at 4°C. BioLegend´s Ultra-Streptavidin (USA) HRP kit (Multi-Species, DAB, Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 20X objective. Scale bar: 50 µm

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835601

100 µL

300€

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835602

500 µL

539€

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Description

Neurofilaments (NFs) are approximately 10 nanometer intermediate filaments found in neurons. They are a major component of the neuronal cytoskeleton, and function primarily to provide structural support for the axon and to regulate the axon diameter. There are three major NF subunits, and the names given to these subunits are based upon the apparent molecular mass of the mammalian subunits on SDS-PAGE. The light or lowest neurofilament (NF-L) runs at 68-70 kD. The medium or middle (NF-M) runs at about 145-160 kD, and the heavy or highest (NF-H) runs at 200-220 kD. However, the actual molecular weight of these proteins is considerably lower due to the highly charged C-terminal regions of the molecules. The level of NF gene expression correlates with the axonal diameter, which controls how fast electrical signals travel down the axon. Mutant mice with NF abnormalities have phenotypes resembling amyotrophic lateral sclerosis. NF immunostaining is common in diagnostic neuropathology. It is useful for differentiating neurons (positive for NF) from the glia (negative for NF).

Product Details

Technical Data Sheet (pdf)

Product Details

Verified Reactivity: Human, Mouse, Rat
Antibody Type: Monoclonal
Host Species: Mouse
Preparation: Ascites
Concentration: The concentration is not quantified as this product is sold as undiluted crude mouse ascites fluid. The concentration might vary from lot-to-lot and an estimated concentration would be 1-3 mg/ml.
Storage & Handling: Store at -20°C. Upon initial thawing, apportion into working aliquots and store at -20°C. Avoid repeated freeze-thaw cycles to prevent denaturing the antibody. For long-term storage, keep the antibody at -80°C.
Application: IHC-P - Quality tested
WB - Verified
IHC-F, ELISA - Reported in the literature, not verified in house
Recommended Usage: Each lot of this antibody is quality control tested by formalin-fixed paraffin-embedded immunohistochemical staining. For immunohistochemistry, a concentration range of 1:1000 - 1:2000 µg/ml is suggested. For Western blotting, the suggested use of this reagent is 1:1000 - 1:5000 µg per ml. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes: Additional reported applications (for the relevant formats of this clone): include ELISA Capture^1-3, immunohistochemical staining on frozen tissue sections, immunofluorescence staining, and spatial biology (IBEX)^6,7.

Clone SMI 35 reacts with highly phosphorylated neurofilaments, as well as with low degrees of phosphorylation. It primarily reacts with neurofilament H and with neurofilament M to a lesser extent.

Notes: On two dimensional gels, this antibody detects a band extending from the phosphorylated neurofilament position at 200 kD (pI 5.1) toward the non-phosphorylated position at 170 kD (pI 6.2).
RRID: AB_2565349 (BioLegend Cat. No. 835601)
AB_2565349 (BioLegend Cat. No. 835602)

Antigen Details

Structure: The medium or middle NF (NF-M) runs at about 145-160 kD, and the heavy or highest NF (NF-H) runs at 200-220 kD.
Distribution: Tissue Distribution: CNS, peripheral nerves and glandular cells of the prostate
Cellular Distribution: Cytoskeleton, nucleus, cytosol, and mitochondrion
Function: Neurofilaments are the major components of the neuronal cytoskeleton. They provide axonal support and regulate axon diameter.
Interaction: Cell bodies and dendrites are generally unstained. Other cells and tissues are unreactive except for peripheral axons.
Cell Type: Mature Neurons
Biology Area: Cell Biology, Neuroscience, Neuroscience Cell Markers
Molecular Family: Intermediate Filaments, Phospho-Proteins
Antigen References: Petzold A. 2012. Mult Scler Int. 2012:217802. PubMed

Gresle MM, et al. 2011. Mult Scler Int. 2011: 315406. PubMed
Gene ID: 4744 View all products for this Gene ID
UniProt: View information about Neurofilament HM NF-H NF-M Phospho on UniProt.org

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Other Formats

View All Neurofilament H&M (NF-H/NF-M), Phospho Reagents Request Custom Conjugation

Description	Clone	Applications
Anti-Neurofilament H & M (NF-H/NF-M), Hypophosphorylated	SMI 35	IHC-P,WB,IHC-F,ELISA
Purified anti-Neurofilament H & M (NF-H/NF-M), Hypophosphorylated	SMI 35	IHC-P,WB,IHC-F
Biotin anti-Neurofilament H & M (NF-H/NF-M), Hypophosphorylated	SMI 35	WB
Alexa Fluor® 594 anti-Neurofilament H & M (NF-H/NF-M), Hypophosphorylated	SMI 35	IHC-P,ICC
Alexa Fluor® 647 anti NF-H/NF-M, Hypophosphorylated	SMI 35	IHC-P
Alexa Fluor® 488 anti-Neurofilament H & M (NF-H/NF-M), Hypophosphorylated	SMI 35	IHC-P,SB

Certificate of Analysis

Go To Top Version: 4 Revision Date: 10.16.2018

For Research Use Only. Not for diagnostic or therapeutic use.

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

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