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APC anti-human CD157 (BST-1) Antibody

Pricing & Availability
Clone
W21007F (See other available formats)
Regulatory Status
RUO
Other Names
BST1
Isotype
Rat IgG2a, λ
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Product Citations
publications
W21007F_APC_CD157_APC_2_052623
Human peripheral blood mononuclear cells were stained with anti-human CD123 Alexa Fluor® 700 and anti-human CD157 (BST-1) (clone W21007F) (left) APC or rat IgG2a, λ APC isotype control (right). Data shown was gated on CD45-positive lymphocytes+monocytes.
  • W21007F_APC_CD157_APC_2_052623
    Human peripheral blood mononuclear cells were stained with anti-human CD123 Alexa Fluor® 700 and anti-human CD157 (BST-1) (clone W21007F) (left) APC or rat IgG2a, λ APC isotype control (right). Data shown was gated on CD45-positive lymphocytes+monocytes.
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382109 25 tests 124€
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382110 100 tests 276€
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Description

CD157, also known as Bone Marrow Stromal Cell Antigen 1 (BST1), is a GPI-anchored glycoprotein. It is an ectoenzyme that has both cyclic ADP-ribose hydrolase and ADP-ribosyl cyclase activities. It is prevalently expressed by cells of the myelomonocytic lineage, and modulates adhesion of neutrophils and monocytes to the extracellular matrix. CD157 is expressed in 97% of primary acute myeloid leukemia samples, and is used as a marker for diagnosis of Paroxysmal Nocturnal Hemoglobinuria, a disease caused by intrinsic deficiency of GPI anchors. 

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
hCD157 stable transfectant followed by recombinant protein (aa29-293)
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography and conjugated with APC under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Red Laser (633 nm)
RRID
AB_2941560 (BioLegend Cat. No. 382109)
AB_2941560 (BioLegend Cat. No. 382110)

Antigen Details

Structure
GPI-anchored, ADP-ribosyl cyclase
Distribution

Cell Membrane

Function
ADP-ribosyl cyclase activity. Fibronectin, fibrinogen, laminin-1 and type 1 collagen binding. Hydrolase, Transferase.
Interaction
CD11b/CD18, ECM components
Ligand/Receptor
NAD, NADP
Cell Type
Antigen-presenting cells, Granulocytes, Monocytes
Biology Area
Cell Adhesion, Cell Proliferation and Viability, Innate Immunity
Molecular Family
CD Molecules, Enzymes and Regulators, Innate Immune Signaling
Antigen References
  1. Ortolan E, et al. 2019. Immunology Letters. 205:59-64
  2. Ferrero E, et al. 2017. Sci Rep. 7: 15923.
  3. Kaisho T, et al. 1994. Proc Natl Acad Sci USA. 91:5325-9.
  4. Morone S, et al. 2014. J Biol Chem. 289:15588-601.
Gene ID
683 View all products for this Gene ID
UniProt
View information about CD157 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 1    Revision Date: 05.26.2023

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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