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- Clone
- RM153 (See other available formats)
- Regulatory Status
- RUO
- Other Names
- CD30 ligand, CD30L, TNFSF8
- Isotype
- Rat IgG2b, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
-
PMA+ionomycin-stimulated (2 day) C57BL/6 mouse splenocytes were stained with CD153 (clone RM153) APC (filled histogram) or rat IgG2b, κ APC isotype control (open histogram).
| Cat # | Size | Price | Quantity Check Availability | Save | ||
|---|---|---|---|---|---|---|
| 106409 | 50 µg | 105€ | ||||
CD153 is a 39-40 kD type II membrane glycoprotein, also known as CD30 ligand or CD30L. This TNF superfamily member is expressed on activated T cells. CD153 has been reported to stimulate B-cell proliferation, antigen-specific antibody production, polyclonal immunoglobulin secretion, and T cell activation and proliferation. The RM153 antibody inhibits binding of CD153 to CD30.
Product DetailsProduct Details
- Verified Reactivity
- Mouse
- Antibody Type
- Monoclonal
- Host Species
- Rat
- Immunogen
- CHO cells transfected with mouse CD153 gene (Tnfsf8)
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography and conjugated with APC under optimal conditions.
- Concentration
- 0.2 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
FC - Quality tested
- Recommended Usage
-
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
- Excitation Laser
-
Red Laser (633 nm)
- Application Notes
-
Additional reported applications (for the relevant formats) include: immunoprecipitation, immunohistochemical staining of acetone-fixed frozen sections, and in vivo blocking of CD30 binding to CD30 ligand (CD153)1-3,5. The LEAF™ purified antibody (Endotoxin <0.1 EU/μg, Azide-Free, 0.2 μm filtered) is recommended for functional assays (Cat. No. 106408).
- Application References
- RRID
-
AB_2562680 (BioLegend Cat. No. 106409)
Antigen Details
- Structure
- TNF superfamily, 39-40 kD
- Distribution
- Activated T cells
- Function
- T-B cell costimulation
- Ligand/Receptor
- CD30
- Cell Type
- T cells
- Biology Area
- Immunology
- Molecular Family
- CD Molecules
- Antigen References
-
1. Barclay A, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Horie R, et al. 1998. Semin. Immunol. 106:457.
3. Shimozato O, et al. 1999. Biochem. Biophys. Res. Commun. 256:519.
4. Shanebeck KD, et al. 1995. Eur. J. Immunol. 25:2147.
5. Bowen MA, et al. 1996. J. Immunol. 156:442. - Gene ID
- 21949 View all products for this Gene ID
- UniProt
- View information about CD153 on UniProt.org
Related Pages & Pathways
Pathways
Other Formats
View All CD153 Reagents Request Custom Conjugation| Description | Clone | Applications |
|---|---|---|
| PE anti-mouse CD153 | RM153 | FC |
| APC anti-mouse CD153 | RM153 | FC |
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Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
-
PE anti-mouse CD153
TPA-stimulated (20 hrs) splenocytes stained with purified RM... -
APC anti-mouse CD153
PMA+ionomycin-stimulated (2 day) C57BL/6 mouse splenocytes w...
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