APC anti-Phosphotyrosine Antibody anti-Phosphotyrosine

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Clone: PY20 (See other available formats)
Regulatory Status: RUO
Other Names: p-Tyr
Isotype: Mouse IgG2b, κ
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Product Citations: publications

PY20_APC_Phosphotyrosine_Antibody_032116 — Human T leukemia cell line Jurkat was cultured in serum-free media overnight and treated with 5mM H₂O₂ (filled histogram) or PBS (open histogram) for 15 minutes. The cells were then fixed with 2% paraformaldehyde, permeabilized with 90% methanol and stained with anti-phosphotyrosine (clone PY20) APC.

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309312

100 µg

214€

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Description

Phosphorylation is a common modification of proteins that can result in alterations in protein function, protein-protein association, cellular localization, and protein-half life. Phosphorylation can occur on threonine, serine, and tyrosine residues. The PY20 monoclonal antibody recognizes phosphorylated tyrosine residues in all species tested (human, mouse, rat, dog, chicken, and frog). The PY20 antibody has been shown to be useful for flow cytometry, immunoprecipitation, Western blotting, and immunofluorescence staining.

Product Details

Technical Data Sheet (pdf)

Product Details

Verified Reactivity: Human, Mouse, Rat, All Species
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: KLH-conjugated phosphotyrosine
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography and conjugated with APC under optimal conditions.
Concentration: 0.2 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: ICFC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
Excitation Laser: Red Laser (633 nm)
Application Notes: Additional reported applications (for the relevant formats) include: immunoprecipitation^1,2, Western blotting^1,2, immunocytochemistry³.
RRID: AB_2572197 (BioLegend Cat. No. 309312)

Antigen Details

Distribution: Phospho-Specific
Function: Phosphorylation of specific tyrosine residues, signal transduction, cell cycle progression, oncogenic transformation
Biology Area: Cell Biology, Immunology, Signal Transduction
Molecular Family: Phospho-Proteins, Protein Kinases/Phosphatase
Gene ID: 5800 View all products for this Gene ID
UniProt: View information about Phosphotyrosine on UniProt.org

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Other Formats

View All Phosphotyrosine Reagents Request Custom Conjugation

Description	Clone	Applications
Biotin anti-Phosphotyrosine	PY20	WB
Purified anti-Phosphotyrosine	PY20	WB,ICC,ICFC,IP
Alexa Fluor® 488 anti-Phosphotyrosine	PY20	ICFC
Alexa Fluor® 647 anti-Phosphotyrosine	PY20	ICFC
APC anti-Phosphotyrosine	PY20	ICFC
PE anti-Phosphotyrosine	PY20	ICFC
Alexa Fluor® 594 anti-Phosphotyrosine	PY20	ICC

Certificate of Analysis

Go To Top Version: 1 Revision Date: 03.24.2016

For Research Use Only. Not for diagnostic or therapeutic use.

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

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