Biotin anti-human IgM Antibody anti-IgM

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Clone: MHM-88 (See other available formats)
Regulatory Status: RUO
Other Names: Immunoglobulin M
Isotype: Mouse IgG1, κ
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Product Citations: publications

Human peripheral blood lymphocytes stained with CD19 APC and biotinylated MHM-88, followed by Sav-PE

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314504

100 µg

90€

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Description

IgM is the first immunoglobulin made by B cells in the immune response. Surface IgM is expressed on immature and mature B cells, while IgM heavy (μ) chain is expressed intracellularly in pre-B cells.

Product Details

Technical Data Sheet (pdf)

Product Details

Verified Reactivity: Human
Reported Reactivity: African Green, Baboon, Cynomolgus, Rhesus
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Human Ig cocktail
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography, and conjugated with biotin under optimal conditions.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C. Do not freeze.
Application: FC - Quality tested
ELISA - Reported in the literature, not verified in house
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.5 µg per 10⁶ in 100 µl staining volume. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes: MHM-88 antibody reacts with both soluble and membrane human immunoglobulin M (IgM). It does not react with other Ig isotypes. Additional reported applications (for the relevant formats) include: use as a primary or secondary reagent for ELISA analysis.

Due to the presence of excess soluble IgM in whole blood, which competes for antibody binding, staining for IgM on cells in whole blood is not recommended.
RRID: AB_493007 (BioLegend Cat. No. 314504)

Antigen Details

Structure: Ig family
Distribution: B cells
Function: B cell differentiation, humoral immune response; cross-linking surface IgM induces apoptosis
Cell Type: B cells
Biology Area: Immunology
Gene ID: 3507 View all products for this Gene ID
UniProt: View information about IgM on UniProt.org

Related Protocols

Cell Surface Flow Cytometry Staining Protocol

Related Products

Description	Clone	Applications

Related FAQs

How many biotin molecules are per antibody structure?: We don't routinely measure the number of biotins with our antibody products but the number of biotin molecules range from 3-6 molecules per antibody.

Other Formats

View All IgM Reagents Request Custom Conjugation

Description	Clone	Applications
Purified anti-human IgM	MHM-88	FC,ELISA
PE anti-human IgM	MHM-88	FC
Biotin anti-human IgM	MHM-88	FC,ELISA
FITC anti-human IgM	MHM-88	FC
APC anti-human IgM	MHM-88	FC
PerCP/Cyanine5.5 anti-human IgM	MHM-88	FC
Pacific Blue™ anti-human IgM	MHM-88	FC
Brilliant Violet 421™ anti-human IgM	MHM-88	FC
APC/Cyanine7 anti-human IgM	MHM-88	FC
Brilliant Violet 570™ anti-human IgM	MHM-88	FC
Brilliant Violet 510™ anti-human IgM	MHM-88	FC
Brilliant Violet 605™ anti-human IgM	MHM-88	FC
Brilliant Violet 650™ anti-human IgM	MHM-88	FC
Purified anti-human IgM (Maxpar® Ready)	MHM-88	FC,CyTOF®
PE/Dazzle™ 594 anti-human IgM	MHM-88	FC
PE/Cyanine7 anti-human IgM	MHM-88	FC
Alexa Fluor® 488 anti-human IgM	MHM-88	FC
Alexa Fluor® 647 anti-human IgM	MHM-88	FC
Alexa Fluor® 700 anti-human IgM	MHM-88	FC
Brilliant Violet 711™ anti-human IgM	MHM-88	FC
TotalSeq™-A0136 anti-human IgM	MHM-88	PG
TotalSeq™-C0136 anti-human IgM	MHM-88	PG
Brilliant Violet 785™ anti-human IgM	MHM-88	FC
APC/Fire™ 750 anti-human IgM	MHM-88	FC
TotalSeq™-B0136 anti-human IgM	MHM-88	PG
Spark Violet™ 423 anti-human IgM Antibody	MHM-88	FC
TotalSeq™-D0136 anti-human IgM	MHM-88	PG
Spark Blue™ 550 anti-human IgM	MHM-88	FC
Spark Blue™ 515 anti-human IgM	MHM-88	FC
PE/Fire™ 700 anti-human IgM	MHM-88	FC
Spark YG™ 593 anti-human IgM	MHM-88	FC
PE/Fire™ 640 anti-human IgM	MHM-88	FC
Spark Red™ 718 anti-human IgM (Flexi-Fluor™)	MHM-88	FC
APC anti-human IgM	MHM-88	FC
PE anti-human IgM	MHM-88	FC
Brilliant Violet 750™ anti-human IgM	MHM-88	FC

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Go To Top Version: 3 Revision Date: 10.10.2014

For Research Use Only. Not for diagnostic or therapeutic use.

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