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Brilliant Violet 421™ anti-mouse CD63 Antibody

Pricing & Availability
Clone
NVG-2 (See other available formats)
Regulatory Status
RUO
Other Names
LIMP, LAMP-3, gp55, melanoma-associated antigen, ME491
Isotype
Rat IgG2a, κ
Ave. Rating
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Product Citations
publications
a. NVG-2_BV-421_CD63_FC_1_021125
Thioglycolate-elicited BALB/c mouse peritoneal macrophages were fixed, permeabilized and intracellularly stained with anti-mouse CD11b (clone M1/70) FITC and anti-mouse CD63 (clone NVG-2) Brilliant Violet 421™ (left) or rat IgG2a, κ Brilliant Violet 421™ isotype control (right).
  • a. NVG-2_BV-421_CD63_FC_1_021125
    Thioglycolate-elicited BALB/c mouse peritoneal macrophages were fixed, permeabilized and intracellularly stained with anti-mouse CD11b (clone M1/70) FITC and anti-mouse CD63 (clone NVG-2) Brilliant Violet 421™ (left) or rat IgG2a, κ Brilliant Violet 421™ isotype control (right).
  • b. NVG-2_BV-421_CD63_FC_2_080924
    Mouse endothelial cells, bEnd.3, were stained with anti-mouse CD63 (clone NVG-2) Brilliant Violet 421™ (filled histogram) or rat IgG2a, κ Brilliant Violet 421™ isotype control (open histogram).
Compare all formats See Brilliant Violet 421™ spectral data
Cat # Size Price Quantity Check Availability Save
143929 50 µg 216€
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Description

CD63, also known as LIMP, LAMP-3, gp55, and melanoma-associated antigen (ME491), is a member of the tetraspanin superfamily (TM4SF) that constitutes a main component of the lysosomal membrane. It is expressed on activated platelets, monocyte/macrophages, endothelium, fibroblasts, osteoblasts, and smooth muscle cells. CD63 may be involved in platelet activation and is thought to function as a transmembrane adaptor protein. CD63 has been shown to associate with CD9, CD81, VLA-3, and VLA-6. In mice, there are two CD63 gene loci, of which only one is functional. CD63 deficient mice are viable, and there is no alteration in the population of immune cells.  A recent report shows that CD63-deficient mice exhibit a significant reduction in both leukocyte rolling and recruitment in a peritonitis model. 

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Intestinal lamina propria light-density cells (enriched with eosinophils)
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography and conjugated with Brilliant Violet 421™ under optimal conditions.
Concentration
0.2 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

ICFC - Quality tested
FC - Verified
Recommended Usage

Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 1.0 µg per million cells in 100 µL volume. For flow cytometric staining, the suggested use of this reagent is ≤ 1.0 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.

Brilliant Violet 421™ excites at 405 nm and emits at 421 nm. The standard bandpass filter 450/50 nm is recommended for detection. Brilliant Violet 421™ is a trademark of Sirigen Group Ltd.


Learn more about Brilliant Violet™.

This product is subject to proprietary rights of Sirigen Inc. and is made and sold under license from Sirigen Inc. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. Patent(s), pending patent applications and foreign equivalents.
Excitation Laser
Violet Laser (405 nm)
Application Notes

Additional reported applications (for the relevant formats) include: Western blotting1 and immunofluorescence1.

Application References
  1. Verjan Garcia N, et al. 2011. J. Immunol. 187:2268. (WB, IF)

Antigen Details

Structure
Tetraspan transmembrane superfamily (TM4SF), type III lysosomal glycoprotein, 53 kD
Distribution

Activated platelets, monocytes, macrophages, endothelium, fibroblasts, osteoclasts, and smooth muscle cells

Function
Platelet activation
Interaction
CD9, CD81, VLA-3, and VLA-6
Cell Type
Endothelial cells, Fibroblasts, Macrophages, Monocytes, Osteoclasts, Platelets
Biology Area
Immunology, Innate Immunity
Molecular Family
CD Molecules
Antigen References

1. Azorsa DO, et al. 1991. Blood 78:280.
2. Kishimoto T, et al. 1997. Leukocyte Typing V1. Oxford University Press New York.
3. Hildreth JE, et al. 1991. Blood 77:121.

Gene ID
12512 View all products for this Gene ID
UniProt
View information about CD63 on UniProt.org

Related FAQs

What is the F/P ratio range of our BV421™ format antibody reagents?

It is lot-specific. On average it ranges between 2-4.

Go To Top Version: 1    Revision Date: 08.09.2024

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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