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Brilliant Violet 421™ anti-human AXL Antibody

Pricing & Availability
Clone
S21005C (See other available formats)
Regulatory Status
RUO
Other Names
ARK, AXL receptor tyrosine kinase, AXL transforming sequence/gene, AXL oncogene, JTK11, Tyro7, tyrosine-protein kinase receptor UFO
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
S21005C_BV421_AXL_Antibody_2_091124
Human peripheral blood mononuclear cells were stained with a lineage cocktail of anti-human CD3/CD19/CD14/CD15 PE, anti-human CD303 (clone 201A) FITC and anti-human AXL (clone S21005C) Brilliant Violet 421™ (left) or mouse IgG1 κ, Brilliant Violet 421™ isotype control (right). Data shown was gated on the lineage negative population.
  • S21005C_BV421_AXL_Antibody_2_091124
    Human peripheral blood mononuclear cells were stained with a lineage cocktail of anti-human CD3/CD19/CD14/CD15 PE, anti-human CD303 (clone 201A) FITC and anti-human AXL (clone S21005C) Brilliant Violet 421™ (left) or mouse IgG1 κ, Brilliant Violet 421™ isotype control (right). Data shown was gated on the lineage negative population.
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386509 25 tests 180€
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386510 100 tests 352€
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Description

AXL, also known as ARK or UFO, is a widely expressed glycoprotein in the TAM (Tyro3/Dtk, AXL, and Mer) receptor tyrosine kinase family. AXL is highly expressed in solid cancers. It is also expressed on a small population of human dendritic cells that share characteristics of classical and plasmacytoid dendritic cells. AXL binds the vitamin K-dependent growth arrest specific 6 (Gas6) protein and induces receptor autophosphorylation and downstream signaling pathways that are related to cell proliferation, migration, or apoptosis. AXL is also involved in hematopoiesis, embryonic development, tumorigenesis, and regulation of testicular functions. Recent studies have also shown that AXL and Mer are both linked to Alzheimer's disease.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography and conjugated with Brilliant Violet 421™ under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.

Brilliant Violet 421™ excites at 405 nm and emits at 421 nm. The standard bandpass filter 450/50 nm is recommended for detection. Brilliant Violet 421™ is a trademark of Sirigen Group Ltd.


Learn more about Brilliant Violet™.

This product is subject to proprietary rights of Sirigen Inc. and is made and sold under license from Sirigen Inc. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. Patent(s), pending patent applications and foreign equivalents.
Excitation Laser
Violet Laser (405 nm)
Application Notes

Clone S21005C recognizes a different epitope compared to clone S21005B.

Clone S21005C does not block GAS6 induced AKT and ERK phosphorylation in H1299 cells.

Antigen Details

Structure
Transmembrane protein with two fibronectin type-III domains, two Ig-like C2-type domains, and one tyrosine kinase domain
Distribution

Highly expressed in solid cancers and also expressed on human blood plasmacytoid dendritic cells and myeloid dendritic cells

Function
Binding of Gas6 induces receptor autophosphorylation and downstream signaling pathways that are related to cell proliferation, migration, or apoptosis. It is involved in hematopoiesis, embryonic development, tumorigenesis, and regulation of testicular functions. Recent studies have shown that AXL and Mer are both linked to Alzheimer's disease.
Ligand/Receptor
Gas6 and Protein S
Cell Type
Dendritic cells, NK cells
Biology Area
Apoptosis/Tumor Suppressors/Cell Death, Cardiovascular Biology, Cell Death, Cell Proliferation and Viability, Immuno-Oncology, Signal Transduction
Molecular Family
Cytokine/Chemokine Receptors, Cytokines/Chemokines
Antigen References
  1. Holland S, et al. 2005. Canc Res. 65:9294-303.
  2. Lee WP, et al. 2002. Oncogene. 21:329-36.
  3. Demarchi F, et al. 2001. J Biol Chem. 276:31738-44.
Gene ID
558 View all products for this Gene ID
UniProt
View information about AXL on UniProt.org

Related FAQs

What is the F/P ratio range of our BV421™ format antibody reagents?

It is lot-specific. On average it ranges between 2-4.

Go To Top Version: 1    Revision Date: 09.10.2024

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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