Loading...

Brilliant Violet 421™ anti-human HLA-E Antibody

Pricing & Availability
Clone
3D12 (See other available formats)
Regulatory Status
RUO
Other Names
HLA class I histocompatibility antigen, alpha chain E, HLAE
Isotype
Mouse IgG1, κ
Ave. Rating
Submit a Review
Product Citations
publications
3D12_BV421_HLA-E_Antibody_120117
Human peripheral blood lymphocytes were stained with HLA-E (clone 3D12) Brilliant Violet 421™ (filled histogram) or mouse IgG1, κ Brilliant Violet 421™ isotype control (open histogram).
  • 3D12_BV421_HLA-E_Antibody_120117
    Human peripheral blood lymphocytes were stained with HLA-E (clone 3D12) Brilliant Violet 421™ (filled histogram) or mouse IgG1, κ Brilliant Violet 421™ isotype control (open histogram).
Compare all formats See Brilliant Violet 421™ spectral data
Cat # Size Price Quantity Check Availability Save
342611 25 tests 144€
Check Availability


Need larger quantities of this item?
Request Bulk Quote
342612 100 tests 309€
Check Availability


Need larger quantities of this item?
Request Bulk Quote
Description

HLA-E is a non-classical MHC class I (MHC-Ib) molecule. It is characterized by limited polymorphism and ubiquitous expression. HLA-E, as other MHC-I molecules, is heterodimerized with β2-microglobulin. HLA-E interacts with CD94/NKG2 receptor to regulate NK cell cytolysis activities.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA).
Preparation
The antibody was purified by affinity chromatography and conjugated with Brilliant Violet 421™ under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

Brilliant Violet 421™ excites at 405 nm and emits at 421 nm. The standard bandpass filter 450/50 nm is recommended for detection. Brilliant Violet 421™ is a trademark of Sirigen Group Ltd.


Learn more about Brilliant Violet™.

This product is subject to proprietary rights of Sirigen Inc. and is made and sold under license from Sirigen Inc. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. Patent(s), pending patent applications and foreign equivalents.
Excitation Laser
Violet Laser (405 nm)
Application References
  1. Lee N, et al. 1998. Proc. Natl. Acad. Sci. USA. 95:5199.
  2. Wooden SL, et al. 2005. J. Immunol. 175:1383.
  3. Monaco EL, et al. 2008. J. Immunol. 181:5442.
  4. Corrah TW, et al. 2011. J. Virol. 85:3367. PubMed
Product Citations
  1. Sun W, et al. 2023. Nature. 614:309. PubMed
  2. Zhuang X, et al. 2020. Front Immunol. 2.415972222. PubMed
  3. van Stigt Thans T, et al. 2020. Journal of Virology. 93(20):e00719-19.. PubMed
  4. Van der Meer JMR, et al. 2021. Oncoimmunology. 10:1981049. PubMed
RRID
AB_2721525 (BioLegend Cat. No. 342611)
AB_2721525 (BioLegend Cat. No. 342612)

Antigen Details

Structure
HLA family, Ig superfamily, 42 kD
Distribution

Broadly expressed

Function
Mediate cell recognition by NK cells
Ligand/Receptor
CD94/NKG2
Biology Area
Immunology
Molecular Family
MHC Antigens
Antigen References

1. Sullivan LC, et al. 2008. Tissue Antigen. 72:415.
2. Koller BH, et al. 1988. J. Immunol. 141:897.

Gene ID
3133 View all products for this Gene ID
UniProt
View information about HLA-E on UniProt.org

Related FAQs

What is the F/P ratio range of our BV421™ format antibody reagents?

It is lot-specific. On average it ranges between 2-4.

Go To Top Version: 0    Revision Date: 12.01.2017

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

ProductsHere
Login / Register
Remember me
Forgot your password? Reset password?
Create an Account