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Brilliant Violet 421™ anti-mouse CD185 (CXCR5) Antibody

Pricing & Availability
Clone
L138D7 (See other available formats)
Regulatory Status
RUO
Other Names
BLR1, GPCR6, MDR-15
Isotype
Rat IgG2b, κ
Ave. Rating
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Product Citations
publications
L138D7_BV421_CXCR5_Antibody_FC_1_052213
C57BL/6 mouse splenocytes were stained with CD45R/B220 FITC and CXCR5 (clone L138D7, top) Brilliant Violet 421™ or rat IgG2b, κ Brilliant Violet 421™ isotype control (bottom).
  • L138D7_BV421_CXCR5_Antibody_FC_1_052213
    C57BL/6 mouse splenocytes were stained with CD45R/B220 FITC and CXCR5 (clone L138D7, top) Brilliant Violet 421™ or rat IgG2b, κ Brilliant Violet 421™ isotype control (bottom).
  • L138D7_BV421_CXCR5_Antibody_FC_2_052213
Compare all formats See Brilliant Violet 421™ spectral data
Cat # Size Price Quantity Check Availability Save
145511 125 µL 150€
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145512 500 µL 331€
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Description

CD185 is also known as CXCR5. It is the receptor for chemokine CXCL13/BLC, which is chemotactic for B cells.  CXCR5 is expressed on B cells and a subset of T cells in the spleen, neuronal tissue, lymph nodes, and bone marrow.  It is important for migration of B cells into the B cell follicles of the spleen and Peyer’s patches.  Follicular helper T cells (Tfh) also express CXCR5 and the ability of these cells to migrate to the lymph node is modulated by the balanced expression of CCR7 and CXCR5.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
mCXCR5-transfected cells
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA).
Preparation
The antibody was purified by affinity chromatography and conjugated with Brilliant Violet 421™ under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.

Brilliant Violet 421™ excites at 405 nm and emits at 421 nm. The standard bandpass filter 450/50 nm is recommended for detection. Brilliant Violet 421™ is a trademark of Sirigen Group Ltd.


Learn more about Brilliant Violet™.

This product is subject to proprietary rights of Sirigen Inc. and is made and sold under license from Sirigen Inc. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. Patent(s), pending patent applications and foreign equivalents.
Excitation Laser
Violet Laser (405 nm)
Application Notes

Clone L138D7 staining works optimally at room temperature or 4°C. Unlike other chemokine receptor antibodies, avoid using L138D7 at 37°C.

Product Citations
  1. Wrzner K, et al. 2021. EBioMedicine. 63:103197. PubMed
  2. Jiang W, et al. 2021. Sci Rep. 11:1864. PubMed
  3. Chaurio RA, et al. 2022. Immunity. 55:115. PubMed
  4. Koenig A, et al. 2022. Front Immunol. 12:791100. PubMed
  5. Oishi K, et al. 2022. Cell Rep. 39:111002. PubMed
  6. Ludwig J, et al. 2023. NPJ Vaccines. 8:52. PubMed
  7. Zhang YN, et al. 2023. Nat Commun. 14:1985. PubMed
  8. Wilfahrt D, et al. 2021. Elife. 10:. PubMed
  9. Hill DL, et al. 2021. Elife. 10:. PubMed
  10. Chappaz S, et al. 2021. Cell Reports. 36(3):109430. PubMed
  11. Vanderleyden I, et al. 2020. Cell Rep. 30:611. PubMed
  12. Kim CJ, et al. 2018. Immunity. 49:1034. PubMed
  13. Ersching J et al. 2017. Immunity. 46(6):1045-1058 . PubMed
  14. Webb LMC, et al. 2021. Aging Cell. 20:e13295. PubMed
  15. Sato R, et al. 2020. Int Immunol. 499:32. PubMed
  16. Snell LM, et al. 2018. Immunity. 49:678. PubMed
  17. Yue X, et al. 2019. Nat Commun. 10:2011. PubMed
  18. Vogel AB, et al. 2021. Nature. 592:283. PubMed
  19. Chauveau L, et al. 2021. EMBO Rep. 22:e52447. PubMed
  20. Zhang YN, et al. 2021. Sci Adv. 7:eabj3107. PubMed
  21. Luck H, et al. 2019. Nat Commun. 10:3650. PubMed
  22. Dheilly E, et al. 2020. Cancer Cell. 37(5):674-689. PubMed
  23. Di Pilato M, et al. 2021. Cell. 184(17):4512-4530.e22. PubMed
  24. Smith LK, et al. 2021. Elife. 10:. PubMed
  25. Lederer K, et al. 2020. Immunity. 53(6):1281-1295.e5. PubMed
  26. Trindade BC, et al. 2021. Immunity. 54:2273. PubMed
RRID
AB_2562127 (BioLegend Cat. No. 145511)
AB_2562127 (BioLegend Cat. No. 145512)

Antigen Details

Structure
42 kD, seven transmembrane protein
Distribution

B cells and a subset of T cells in mouse spleen, Tfh cells, also expressed in lymph node, bone marrow, and brain

Function
B cell migration into follicles, possible regulatory role in B cell differentiation and Burkitt's lymphoma
Ligand/Receptor
BLC/CXCL13
Cell Type
B cells, T cells, Tfh
Biology Area
Cell Biology, Immunology, Innate Immunity, Neuroinflammation, Neuroscience
Molecular Family
CD Molecules, Cytokine/Chemokine Receptors, GPCR
Antigen References

1. Kaiser E, et al. 1993. Eur. J. Immunol. 23:2532.
2. Forster R, et al. 1994. Cell. Mol. Biol. 40:381.
3. Forster R, et al. 1994. Blood 84:830.
4. Forster R, et al. 1996. Cell 87:1037.
5. Wolf I, et al. 1998. J. Biol. Chem. 273:28831.

Gene ID
12145 View all products for this Gene ID
UniProt
View information about CD185 on UniProt.org

Related FAQs

What is the F/P ratio range of our BV421™ format antibody reagents?

It is lot-specific. On average it ranges between 2-4.

Go To Top Version: 1    Revision Date: 06.05.2013

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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