Brilliant Violet 421™ anti-mouse CD365 (Tim-1) Antibody, CD365, RMT1-4

Pricing & Availability

Clone: RMT1-4 (See other available formats)
Regulatory Status: RUO
Other Names: T cell immunoglobulin and mucin domain containing protein-1, T cell and airway phenotype regulator (Tapr), hepatitic virus cellular receptor 1, CD365
Isotype: Rat IgG2b, κ
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RMT1-4_BV-421_CD365_FC_062424 — Mouse Tim-1 transfected cells were stained with anti-mouse CD365 (Tim-1) (clone RMT1-4) Brilliant Violet 421™ (filled histogram) or rat IgG2b, κ Brilliant Violet 421™ isotype control (open histogram)

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119507

50 µg

216€

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Description

CD365 (Tim-1) is a transmembrane protein also known as T cell immunoglobulin and mucin domain containing protein-1 and hepatitis virus cellular receptor 1. It is developmentally expressed at high levels in the blastocyst. Tim-1 is expressed on activated CD4⁺ lymphocytes especially on Th₂ cells and has been implicated to play a critical role in the development of atopic disease and other Th₂-biased immune responses. Tim-1 is hepatitis A virus receptor in humans. Tim-4 is the endogenous ligand of Tim-1. The interaction of Tim-1 and Tim-4 is involved in costimulation of T cell proliferation. Tim-1 is an endogenous ligand for LMIR5/CD300b.

Product Details

Technical Data Sheet (pdf)

Product Details

Verified Reactivity: Mouse
Antibody Type: Monoclonal
Host Species: Rat
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation: The antibody was purified by affinity chromatography and conjugated with Brilliant Violet 421™ under optimal conditions.
Concentration: 0.2 mg/mL
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.5 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.

Brilliant Violet 421™ excites at 405 nm and emits at 421 nm. The standard bandpass filter 450/50 nm is recommended for detection. Brilliant Violet 421™ is a trademark of Sirigen Group Ltd.

Learn more about Brilliant Violet™.

This product is subject to proprietary rights of Sirigen Inc. and is made and sold under license from Sirigen Inc. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. Patent(s), pending patent applications and foreign equivalents.
Excitation Laser: Violet Laser (405 nm)
RRID: AB_3662394 (BioLegend Cat. No. 119507)

Antigen Details

Structure: Transmembrane protein containing immunoglobulin domain and mucin-like domain; predicted molecular weight 33 kD.
Distribution: Developmentally regulated; highly expressed in embryonic development in blastocysts. Expressed on activated CD4⁺ lymphocytes, especially Th2 cell lines.
Function: Binds hepatitis virus, may play a critical role in immune responses and the development of atopic diseases (in particular airway hyperreactivity).
Ligand/Receptor: Binds hepatitis A virus in humans, Tim-4, LMIR5/CD300b
Cell Type: T cells, Th2
Biology Area: Immunology, Inhibitory Molecules
Molecular Family: CD Molecules
Antigen References: 1. McIntire JJ, et al. 2001. Nature Immunol. 2:1109.
2. Kuchroo VK, et al. 2003. Nat. Rev. Immunol. 3:454.
3. Wills-Karp M, et al. 2001. Nat. Rev. Immunol. 1:69.
4. Meyers JH, et al. 2005. Nat. Immunol. 6(5):455.
Gene ID: 171283 View all products for this Gene ID
UniProt: View information about CD365 on UniProt.org

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Cell Surface Flow Cytometry Staining Protocol

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Related FAQs

What is the F/P ratio range of our BV421™ format antibody reagents?: It is lot-specific. On average it ranges between 2-4.

Other Formats

View All CD365 Reagents Request Custom Conjugation

Description	Clone	Applications
Purified anti-mouse CD365 (Tim-1)	RMT1-4	FC
Biotin anti-mouse CD365 (Tim-1)	RMT1-4	FC
PE anti-mouse CD365 (Tim-1)	RMT1-4	FC
Brilliant Violet 421™ anti-mouse CD365 (Tim-1)	RMT1-4	FC

Certificate of Analysis

Go To Top Version: 1 Revision Date: 06.24.2024

For Research Use Only. Not for diagnostic or therapeutic use.

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

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