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Brilliant Violet 421™ Rat IgG2b, κ Isotype Ctrl Antibody

Pricing & Availability
Clone
RTK4530 (See other available formats)
Regulatory Status
RUO
Isotype
Rat IgG2b, κ
Ave. Rating
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Product Citations
publications
Compare all formats See Brilliant Violet 421™ spectral data
Cat # Size Price Quantity Check Availability Save
400639 125 µL 132€
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400655 50 µg 199€
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400640 500 µL 287€
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Description

The isotype of RTK4530 immunoglobulin is rat IgG2b, κ. This antibody was chosen as an isotype control after screening on a variety of resting, activated, live, and fixed mouse, rat and human tissues.

Product Details
Technical Data Sheet (pdf)

Product Details

Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Trinitrophenol + KLH
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA).
Preparation
The immunoglobulin was purified by affinity chromatography and conjugated with Brilliant Violet 421™ under optimal conditions.
Concentration
µg sizes: 0.2 mg/mL
µL sizes: lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested

ICFC - Verified
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis as negative control. Use at concentrations comparable to those of the specific antibody of interest.

Brilliant Violet 421™ excites at 405 nm and emits at 421 nm. The standard bandpass filter 450/50 nm is recommended for detection. Brilliant Violet 421™ is a trademark of Sirigen Group Ltd.


Learn more about Brilliant Violet™.

This product is subject to proprietary rights of Sirigen Inc. and is made and sold under license from Sirigen Inc. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. Patent(s), pending patent applications and foreign equivalents.
Excitation Laser
Violet Laser (405 nm)
Application Notes

The RTK4530 immunoglobulin is useful as an isotype-matched control (for the relevant formats) for Western blotting, immunoprecipitation, immunohistochemistry, functional assay, and immunofluorescence microscopy. The Ultra-LEAF™ purified antibody (Endotoxin <  0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 400643, 400644, 400671, 400672, 400675, and 400676) as negative control.

Application References
  1. Cervantes-Barragan L, et al. 2007. Blood 109:1131.
  2. Zeiser R, et al. 2007. Blood 109:2225.
  3. Sasaki K, et al. 2008. J. Immunol. 181:104. PubMed
  4. Duan J, et al. 2008. P. Natl. Acad. Sci. USA 105:5183. PubMed
  5. Yi H, et al. 2009. Blood 113:5819. PubMed
  6. Schafeer JS, et al. 2010. J. Leukocyte Biol. 87:301. PubMed
  7. Lei GS, et al. 2015. Infect Immun. 83:572. PubMed
  8. Richards J, et al. 2015. Mol Cell Cardiol. 79:21. PubMed
Product Citations
  1. Alam A, et al. 2022. Cancer Cell. 40:153. PubMed
  2. Chithanathan K, et al. 2022. Cells. 11: . PubMed
  3. Li B, Schmidt N 2016. PLoS One. 11: 0162427. PubMed
  4. Baram T, et al. 2021. Cells. 10:. PubMed
  5. Yan L, et al. 2021. Front Cell Neurosci. 15:750373. PubMed
  6. Girousse A et al. 2019. Cell Rep. 27(2):323-333 . PubMed
  7. Joseph R, et al. 2021. Br J Cancer. 125:176. PubMed
  8. Giesen D, et al. 2020. Clin Cancer Res. 26:3999. PubMed
  9. Piirainen S, et al. 2021. Glia. 69:2459. PubMed
  10. O'Reilly LA, et al. 2018. Immunity. 48:570. PubMed
  11. Gross KM, et al. 2019. Cell Rep. 28:394. PubMed
RRID
AB_10895758 (BioLegend Cat. No. 400639)
AB_10895758 (BioLegend Cat. No. 400655)
AB_11204247 (BioLegend Cat. No. 400640)

Antigen Details

Gene ID
NA

Related FAQs

What is the F/P ratio range of our BV421™ format antibody reagents?

It is lot-specific. On average it ranges between 2-4.

Other Formats

View All Reagents Request Custom Conjugation
Description Clone Applications
APC Rat IgG2b, κ Isotype Ctrl RTK4530 FC,ICFC
Biotin Rat IgG2b, κ Isotype Ctrl RTK4530 FC,ICFC
FITC Rat IgG2b, κ Isotype Ctrl RTK4530 FC,ICFC
PE Rat IgG2b, κ Isotype Ctrl RTK4530 FC,ICFC
PE/Cyanine5 Rat IgG2b, κ Isotype Ctrl RTK4530 FC,ICFC
Purified Rat IgG2b, κ Isotype Ctrl RTK4530 FC,ICFC,ICC,IHC,IP,WB
PE/Cyanine7 Rat IgG2b, κ Isotype Ctrl RTK4530 FC,ICFC
APC/Cyanine7 Rat IgG2b, κ Isotype Ctrl RTK4530 FC,ICFC
Alexa Fluor® 647 Rat IgG2b, κ Isotype Ctrl RTK4530 FC,ICFC
Alexa Fluor® 488 Rat IgG2b, κ Isotype Ctrl RTK4530 FC,ICFC
Pacific Blue™ Rat IgG2b, κ Isotype Ctrl RTK4530 FC,ICFC
Alexa Fluor® 700 Rat IgG2b, κ Isotype Ctrl RTK4530 FC,ICFC
PerCP Rat IgG2b, κ Isotype Ctrl RTK4530 FC,ICFC
PerCP/Cyanine5.5 Rat IgG2b, κ Isotype Ctrl RTK4530 FC,ICFC
Brilliant Violet 421™ Rat IgG2b, κ Isotype Ctrl RTK4530 FC,ICFC
Brilliant Violet 570™ Rat IgG2b, κ Isotype Ctrl RTK4530 FC,ICFC
Ultra-LEAF™ Purified Rat IgG2b, κ Isotype Ctrl RTK4530 FC,ICFC,ICC,IHC,IP,WB,FA
Brilliant Violet 510™ Rat IgG2b, κ Isotype Ctrl RTK4530 FC,ICFC
Brilliant Violet 605™ Rat IgG2b, κ Isotype Ctrl RTK4530 FC,ICFC
Brilliant Violet 785™ Rat IgG2b, κ Isotype Ctrl RTK4530 FC,ICFC
Brilliant Violet 650™ Rat IgG2b, κ Isotype Ctrl RTK4530 FC,ICFC
Brilliant Violet 711™ Rat IgG2b, κ Isotype Ctrl RTK4530 FC,ICFC
PE/Dazzle™ 594 Rat IgG2b, κ Isotype Ctrl RTK4530 FC
Alexa Fluor® 594 Rat IgG2b, κ Isotype Ctrl RTK4530 IHC-F
GoInVivo™ Purified Rat IgG2b, κ Isotype Ctrl RTK4530 FC,ICFC,ICC,IHC,IP,WB,FA
APC/Fire™ 750 Rat IgG2b, κ Isotype Ctrl RTK4530 FC,ICFC
TotalSeq™-A0095 Rat IgG2b, κ Isotype Ctrl RTK4530 PG
TotalSeq™-C0095 Rat IgG2b, κ Isotype Ctrl RTK4530 PG
KIRAVIA Blue 520™ Rat IgG2b, κ Isotype Ctrl RTK4530 FC
Spark Blue™ 550 Rat IgG2b, κ Isotype Ctrl RTK4530 FC,ICFC
PE/Fire™ 640 Rat IgG2b, κ Isotype Ctrl RTK4530 FC,ICFC
APC/Fire™ 810 Rat IgG2b, κ Isotype Ctrl RTK4530 FC,ICFC
PE/Fire™ 700 Rat IgG2b, κ Isotype Ctrl RTK4530 FC,ICFC
TotalSeq™-B0095 Rat IgG2b, κ Isotype Ctrl RTK4530 PG
TotalSeq™-D0095 Rat IgG2b, κ Isotype Ctrl RTK4530 PG
PE/Fire™ 810 Rat IgG2b, κ Isotype Ctrl RTK4530 FC
Brilliant Violet 750™ Rat IgG2b, κ Isotype Ctrl RTK4530 FC
Spark YG™ 570 Rat IgG2b, κ Isotype Ctrl RTK4530 FC
Spark NIR™ 685 Rat IgG2b, κ Isotype Ctrl RTK4530 FC
Spark Blue™ 574 Rat IgG2b, κ Isotype Ctrl RTK4530 FC
PerCP/Fire™ 806 Rat IgG2b, κ Isotype Ctrl RTK4530 FC
Spark Blue™ 515 Rat IgG2b, κ Isotype Ctrl RTK4530 FC,ICFC
PerCP/Fire™ 780 Rat IgG2b, κ Isotype Ctrl RTK4530 FC
Spark Red™ 718 Rat IgG2b, κ Isotype Ctrl RTK4530 FC,ICFC
TotalSeq™-Bn0095 Rat IgG2b, κ Isotype Ctrl RTK4530 SB
Spark Violet™ 538 Rat IgG2b, κ Isotype Ctrl RTK4530 FC
Spark UV™ 387 Rat IgG2b, κ Isotype Ctrl RTK4530 FC
Spark PLUS UV395™ Rat IgG2b, κ Isotype Ctrl RTK4530 FC
PE/Fire™ 744 Rat IgG2b, κ Isotype Ctrl RTK4530 FC
Go To Top Version: 1    Revision Date: 11.30.2012

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

  • APC Rat IgG2b, κ Isotype Ctrl

  • Biotin Rat IgG2b, κ Isotype Ctrl

  • FITC Rat IgG2b, κ Isotype Ctrl

  • PE Rat IgG2b, κ Isotype Ctrl

  • PE/Cyanine5 Rat IgG2b, κ Isotype Ctrl

  • Purified Rat IgG2b, κ Isotype Ctrl

  • PE/Cyanine7 Rat IgG2b, κ Isotype Ctrl

  • APC/Cyanine7 Rat IgG2b, κ Isotype Ctrl

  • Alexa Fluor® 647 Rat IgG2b, κ Isotype Ctrl

  • Alexa Fluor® 488 Rat IgG2b, κ Isotype Ctrl

  • Pacific Blue™ Rat IgG2b, κ Isotype Ctrl

  • Alexa Fluor® 700 Rat IgG2b, κ Isotype Ctrl

    RTK4530_Alx700_080408.jpg
    CD57BL/6 mouse splenocytes stained with RTK4530 Alexa Fluor®...

  • PerCP Rat IgG2b, κ Isotype Ctrl

  • PerCP/Cyanine5.5 Rat IgG2b, κ Isotype Ctrl

  • Brilliant Violet 421™ Rat IgG2b, κ Isotype Ctrl

  • Brilliant Violet 570™ Rat IgG2b, κ Isotype Ctrl

  • Ultra-LEAF™ Purified Rat IgG2b, κ Isotype Ctrl

  • Brilliant Violet 510™ Rat IgG2b, κ Isotype Ctrl

  • Brilliant Violet 605™ Rat IgG2b, κ Isotype Ctrl

  • Brilliant Violet 785™ Rat IgG2b, κ Isotype Ctrl

  • Brilliant Violet 650™ Rat IgG2b, κ Isotype Ctrl

  • Brilliant Violet 711™ Rat IgG2b, κ Isotype Ctrl

  • PE/Dazzle™ 594 Rat IgG2b, κ Isotype Ctrl

  • Alexa Fluor® 594 Rat IgG2b, κ Isotype Ctrl

  • GoInVivo™ Purified Rat IgG2b, κ Isotype Ctrl

  • APC/Fire™ 750 Rat IgG2b, κ Isotype Ctrl

  • TotalSeq™-A0095 Rat IgG2b, κ Isotype Ctrl

  • TotalSeq™-C0095 Rat IgG2b, κ Isotype Ctrl

  • KIRAVIA Blue 520™ Rat IgG2b, κ Isotype Ctrl

  • Spark Blue™ 550 Rat IgG2b, κ Isotype Ctrl

  • PE/Fire™ 640 Rat IgG2b, κ Isotype Ctrl

  • APC/Fire™ 810 Rat IgG2b, κ Isotype Ctrl

  • PE/Fire™ 700 Rat IgG2b, κ Isotype Ctrl

  • TotalSeq™-B0095 Rat IgG2b, κ Isotype Ctrl

  • TotalSeq™-D0095 Rat IgG2b, κ Isotype Ctrl

  • PE/Fire™ 810 Rat IgG2b, κ Isotype Ctrl

  • Brilliant Violet 750™ Rat IgG2b, κ Isotype Ctrl

  • Spark YG™ 570 Rat IgG2b, κ Isotype Ctrl

  • Spark NIR™ 685 Rat IgG2b, κ Isotype Ctrl

  • Spark Blue™ 574 Rat IgG2b, κ Isotype Ctrl

  • PerCP/Fire™ 806 Rat IgG2b, κ Isotype Ctrl

  • Spark Blue™ 515 Rat IgG2b, κ Isotype Ctrl

  • PerCP/Fire™ 780 Rat IgG2b, κ Isotype Ctrl

  • Spark Red™ 718 Rat IgG2b, κ Isotype Ctrl

  • TotalSeq™-Bn0095 Rat IgG2b, κ Isotype Ctrl

  • Spark Violet™ 538 Rat IgG2b, κ Isotype Ctrl

  • Spark UV™ 387 Rat IgG2b, κ Isotype Ctrl

  • Spark PLUS UV395™ Rat IgG2b, κ Isotype Ctrl

  • PE/Fire™ 744 Rat IgG2b, κ Isotype Ctrl

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