Brilliant Violet 570™ anti-mouse CX3CR1 Antibody, CX3CR1, SA011F11

Pricing & Availability

Clone: SA011F11 (See other available formats)
Regulatory Status: RUO
Other Names: Chemokine (C-X3-C motif) receptor 1, Fractalkine receptor, GPR13, CCRL1, CMKBRL1, CMKDR1, V28
Isotype: Mouse IgG2a, κ
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SA011F11_BV570_CX3CR1_Antibody_082923 — C57BL/6 mouse splenocytes were stained with anti-mouse/human CD11b APC and anti-mouse CX3CR1 (clone SA011F11) Brilliant Violet 570™ (left) or mouse IgG2a, κ Brilliant Violet 570™ isotype control (right).

Compare all formats See Brilliant Violet 570™ spectral data

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149061

50 µg

234€

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Description

CX3CR1 is a 40 kD, G-protein coupled receptor, with seven transmembrane regions. CX3CR1 is expressed by resident and alternatively activated macrophages (M2), a subset of monocytes, dendritic cells (DCs), NK cells, a subset of memory T cells, and mast cells. CX3CR1 is involved in cell recruitment during inflammation and participates in cell adhesion and extravasation from blood vessels. Its ligand is CX3CL1, also known as fractalkine or neurotactin. CX3CR1 is also a coreceptor for HIV1 and variations in this gene leads to increased susceptibility to HIV. In the brain, it is expressed by glial cells, which interact with CX3CL1 expressed by neurons.

Product Details

Technical Data Sheet (pdf)

Product Details

Verified Reactivity: Mouse
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Mouse CX3CR1-transfected cells
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation: The antibody was purified by affinity chromatography and conjugated with Brilliant Violet 570™ under optimal conditions.
Concentration: 0.2 mg/mL
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.5 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.

Brilliant Violet 570™ excites at 405 nm and emits at 570 nm. The bandpass filter 585/42 nm is recommended for detection, although filter optimization may be required depending on other fluorophores used. Be sure to verify that your cytometer configuration and software setup are appropriate for detecting this channel. Refer to your instrument manual or manufacturer for support. Brilliant Violet 570™ is a trademark of Sirigen Group Ltd.

Learn more about Brilliant Violet™.

This product is subject to proprietary rights of Sirigen Inc. and is made and sold under license from Sirigen Inc. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. Patent(s), pending patent applications and foreign equivalents.
Excitation Laser: Violet Laser (405 nm)
Application Notes: View more applications data for a different format of this clone in our Scientific Poster Library.
RRID: AB_3083131 (BioLegend Cat. No. 149061)

Antigen Details

Structure: G-protein coupled receptor, 7 transmembrane regions, 40 kD.
Distribution: Resident and alternatively activated macrophages (M2), subset of monocytes, dendritic cells (DCs), natural killer cells (NK), subset of memory T cells, mast cells, glial cells, and astrocytes.
Function: Involved in cell recruitment during inflammation, participates in cell adhesion and extravasation from blood vessels, coreceptor for HIV1.
Ligand/Receptor: CX3CL1 (fractalkine, neurotactin).
Cell Type: Astrocytes, Dendritic cells, Macrophages, Mast cells, Microglia, Monocytes, NK cells, T cells
Biology Area: Cell Adhesion, Cell Biology, Immunology, Innate Immunity, Neuroscience, Neuroscience Cell Markers
Molecular Family: Cytokine/Chemokine Receptors, GPCR
Antigen References: 1. Ponzetta A, et al. 2013. J. Immunol. 191:5684.
2. Jacquelin S, et al. 2013. Blood. 122:674.
3. Garcia JA, et al. 2013. J. Immunol. 191:1063.
4. Lee YS, et al. 2013. Cell. 153:413.
5. Shechter R, et al. 2013. Immunity. 38:555.
Gene ID: 13051 View all products for this Gene ID
UniProt: View information about CX3CR1 on UniProt.org

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Cell Surface Flow Cytometry Staining Protocol

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Other Formats

View All CX3CR1 Reagents Request Custom Conjugation

Description	Clone	Applications
Purified anti-mouse CX3CR1	SA011F11	FC
Alexa Fluor® 647 anti-mouse CX3CR1	SA011F11	FC
PE anti-mouse CX3CR1	SA011F11	FC
APC anti-mouse CX3CR1	SA011F11	FC
PerCP/Cyanine5.5 anti-mouse CX3CR1	SA011F11	FC
Brilliant Violet 421™ anti-mouse CX3CR1	SA011F11	FC
Brilliant Violet 510™ anti-mouse CX3CR1	SA011F11	FC
FITC anti-mouse CX3CR1	SA011F11	FC
Alexa Fluor® 488 anti-mouse CX3CR1	SA011F11	FC
PE/Dazzle™ 594 anti-mouse CX3CR1	SA011F11	FC
PE/Cyanine7 anti-mouse CX3CR1	SA011F11	FC
Biotin anti-mouse CX3CR1	SA011F11	FC,Cell Sep - Pos
Brilliant Violet 605™ anti-mouse CX3CR1	SA011F11	FC
Brilliant Violet 785™ anti-mouse CX3CR1	SA011F11	FC
Brilliant Violet 711™ anti-mouse CX3CR1	SA011F11	FC
Brilliant Violet 650™ anti-mouse CX3CR1	SA011F11	FC
Alexa Fluor® 700 anti-mouse CX3CR1	SA011F11	FC
APC/Fire™ 750 anti-mouse CX3CR1	SA011F11	FC
Pacific Blue™ anti-mouse CX3CR1	SA011F11	FC
TotalSeq™-A0563 anti-mouse CX3CR1	SA011F11	PG
TotalSeq™-C0563 anti-mouse CX3CR1	SA011F11	PG
TotalSeq™-B0563 anti-mouse CX3CR1	SA011F11	PG
APC/Cyanine7 anti-mouse CX3CR1 Antibody	SA011F11	FC
PE/Cyanine5 anti-mouse CX3CR1	SA011F11	FC
PE/Fire™ 700 anti-mouse CX3CR1	SA011F11	FC
APC/Fire™ 810 anti-mouse CX3CR1	SA011F11	FC
PE/Fire™ 640 anti-mouse CX3CR1	SA011F11	FC
PE/Fire™ 810 anti-mouse CX3CR1	SA011F11	FC
PerCP/Fire™ 806 anti-mouse CX3CR1	SA011F11	FC
Brilliant Violet 570™ anti-mouse CX3CR1	SA011F11	FC
PerCP/Fire™ 780 anti-mouse CX3CR1	SA011F11	FC
Spark Red™ 718 anti-mouse CX3CR1 (Flexi-Fluor™)	SA011F11	FC
Spark PLUS UV395™ anti-mouse CX3CR1	SA011F11	FC
Spark Blue™ 574 anti-mouse CX3CR1 (Flexi-Fluor™)	SA011F11	FC
Spark Blue™ 550 anti-mouse CX3CR1 (Flexi-Fluor™)	SA011F11	FC

Certificate of Analysis

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Go To Top Version: 1 Revision Date: 08.29.2023

For Research Use Only. Not for diagnostic or therapeutic use.

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

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Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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